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AB271712

Recombinant human PHF8 protein (Active) (DDDDK tag N-Terminus)

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Recombinant human PHF8 protein (Active) (DDDDK tag N-Terminus) is a Human Fragment protein, in the 2 to 525 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KDM7B, KIAA1111, ZNF422, PHF8, Histone lysine demethylase PHF8, Lysine demethylase 7B, PHD finger protein 8, [histone H3]-dimethyl-L-lysine(36) demethylase PHF8, [histone H3]-dimethyl-L-lysine(9) demethylase PHF8

2 Images
Functional Studies - Recombinant human PHF8 protein (Active) (DDDDK tag N-Terminus) (AB271712)
  • FuncS

Unknown

Functional Studies - Recombinant human PHF8 protein (Active) (DDDDK tag N-Terminus) (AB271712)

Specific activity of ab271712 was 0.064 pmol/min/μg.

SDS-PAGE - Recombinant human PHF8 protein (Active) (DDDDK tag N-Terminus) (AB271712)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PHF8 protein (Active) (DDDDK tag N-Terminus) (AB271712)

SDS-PAGE analysis of 3 μg ab271712.

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 0.064 pmol/min/μg

Assay conditions: PHF8 enzyme is incubated with biotinylated histone H3 peptide substrate in HEPES-based buffer in a 10 μg reaction for 1 hr. at RT. Add 5 μg of AlphaLISA™ anti-Rabbit Acceptor beads (20 μg/ml), followed by addition of 5 μl of antibody and 30 min. incubation at RT. Add 10 μg of AlphaScreen™ Streptavidin-conjugated donor beads (40 μg/ml). Alpha counts are measured after 10 min. RT incubation.

Accession

Q9UPP1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Complementary Products

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Recombinant Human WDR5 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human WDR5 protein (AB98079)

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Primary Antibodies

AB280887

Anti-PHF8 antibody [EPR23913-57]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF8 antibody [EPR23913-57] (AB280887)

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Proteins & Peptides

AB198115

Recombinant Human Histone H4 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Histone H4 protein (AB198115)

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AB235805

Recombinant Human MAGEA10 protein (His tag)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"NRSRAIVQRGRVLPPPAPLDTTNLAGRRTLQGRAKMASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSKGHDTHKGKPVKTGSPTFVRELRSRTFDSSDEVILKPTGNQLTVEFLEENSFSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVTRQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVDKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFRFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEIPETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTRPAHSTSVSMSRLSLPSKNG","proteinLength":"Fragment","predictedMolecularWeight":"62 kDa","actualMolecularWeight":null,"aminoAcidEnd":525,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9UPP1","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHF8 also known as Plant Homeodomains Finger Protein 8 is a histone demethylase enzyme. It specifically removes methyl groups from histone H3 at lysine 9 (H3K9me2/me1) and at lysine 27 (H3K27me2/me1). PHF8 is a protein with a molecular mass of around 109 kDa. This protein is expressed widely in various tissues appearing notably in brain tissues and the testis which suggests a broad physiological role.
Biological function summary

PHF8 plays an important role in the regulation of gene expression through chromatin remodeling. It forms part of transcriptional complexes and directly interacts with transcription factors and chromatin proteins. Its activity modulates gene silencing and activation processes impacting multiple cellular functions such as cell cycle progression and neurodevelopment. The activities controlled by PHF8 make it critical for maintaining normal cellular function.

Pathways

PHF8 takes part in integral signaling and epigenetic regulation pathways. It is involved in the MAPK/ERK signaling pathway which affects cell proliferation and differentiation. PHF8 also connects with other proteins such as p53 influencing pathways that govern cell growth and apoptosis. Through these interactions PHF8 helps maintain proper balance in cell cycle and development processes.

Mutations or dysregulation of PHF8 have links to developmental disorders and certain cancers. Specifically PHF8 mutations have associations with X-linked mental retardation and cleft lip/palate conditions. Dysregulated PHF8 activity interacts with proteins like p53 to influence cancer progression particularly in prostate cancer. Understanding PHF8's role aids in the exploration of therapeutic targets for these conditions.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone lysine demethylase with selectivity for mono- and dimethylated residues. It plays an essential role in cell cycle progression and rDNA transcription (PubMed : 19843542, PubMed : 20531378, PubMed : 20548336, PubMed : 20622854). Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks (PubMed : 20101266, PubMed : 20208542, PubMed : 20346720, PubMed : 20622853, PubMed : 20622854). Displays a very low intrinsic activity toward dimethylated H3 'Lys-27' (H3K27Me2) (PubMed : 20346720). May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo (PubMed : 19843542, PubMed : 20023638, PubMed : 20346720). Involved in cell cycle progression by being required to control G1-S transition (PubMed : 20622854). Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes (PubMed : 20531378). Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity : has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3 (PubMed : 20023638, PubMed : 20346720, PubMed : 20421419). Positively modulates transcription of histone demethylase KDM5C, acting synergistically with transcription factor ARX; synergy may be related to enrichment of histone H3K4me3 in regulatory elements (PubMed : 31691806). Required for brain development, probably by regulating expression of neuron-specific genes (By similarity).

Sequence similarities

Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.

Post-translational modifications

Phosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone lysine demethylase with selectivity for mono- and dimethylated residues. It plays an essential role in cell cycle progression and rDNA transcription (PubMed : 19843542, PubMed : 20531378, PubMed : 20548336, PubMed : 20622854). Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks (PubMed : 20101266, PubMed : 20208542, PubMed : 20346720, PubMed : 20622853, PubMed : 20622854). Displays a very low intrinsic activity toward dimethylated H3 'Lys-27' (H3K27Me2) (PubMed : 20346720). May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo (PubMed : 19843542, PubMed : 20023638, PubMed : 20346720). Involved in cell cycle progression by being required to control G1-S transition (PubMed : 20622854). Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes (PubMed : 20531378). Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity : has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3 (PubMed : 20023638, PubMed : 20346720, PubMed : 20421419). Positively modulates transcription of histone demethylase KDM5C, acting synergistically with transcription factor ARX; synergy may be related to enrichment of histone H3K4me3 in regulatory elements (PubMed : 31691806). Required for brain development, probably by regulating expression of neuron-specific genes (By similarity).
See full target information PHF8
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