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AB125556

Recombinant Human PHLPP2 protein

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Recombinant Human PHLPP2 protein is a Human Fragment protein, in the 766 to 1043 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

KIAA0931, PHLPPL, PHLPP2, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPP-like

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SDS-PAGE - Recombinant Human PHLPP2 protein (AB125556)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PHLPP2 protein (AB125556)

SDS PAGE analysis of ab125556 (PHLPP2 protein).

Key facts

Purity

>95% Densitometry

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

Q6ZVD8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"56 kDa","actualMolecularWeight":null,"aminoAcidEnd":1043,"aminoAcidStart":766,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q6ZVD8","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHLPP2 also known as PH domain leucine-rich repeat protein phosphatase 2 functions as a serine/threonine phosphatase that specifically dephosphorylates Akt at the Ser473 site. This regulatory action on Akt influences many cellular processes including cell growth survival and metabolism. PHLPP2 has a molecular mass of approximately 140 kDa and is expressed in various tissues including liver and lung. Alternative names such as SCOP2 may sometimes reference this protein in specific research contexts.
Biological function summary

PHLPP2 modulates key signaling pathways by acting as a significant negative regulator of the Akt signaling pathway. It does not work alone; PHLPP2 can interact with functional complexes to exert its phosphatase activity more effectively. By dephosphorylating Akt PHLPP2 reduces the signaling output that leads to cell proliferation and survival responses leading to normal cellular homeostasis. This regulatory function positions PHLPP2 as a balancing component ensuring that Akt activity remains tightly controlled.

Pathways

PHLPP2 plays a role in the PI3K/Akt signaling pathway which is critical for cellular growth and metabolic functions. It also indirectly impacts the mTOR pathway through its regulation of Akt as Akt is an upstream activator of mTOR. By keeping Akt activity in check via dephosphorylation PHLPP2 keeps the downstream effects of the PI3K/Akt/mTOR axis in balance. It relates closely to proteins like PTEN which also acts as a negative regulator of the PI3K/Akt pathway ensuring that excessive Akt activity does not drive uncontrolled cellular events.

Mutations or altered expression of PHLPP2 have connections to cancer and diabetes. In cancer decreased levels of PHLPP2 can lead to an increase in Akt activity promoting uncontrolled cell division and survival therefore contributing to tumorigenesis. PHLPP2's influence on diabetes involves its regulation of insulin signaling where dysregulation can affect glucose homeostasis. The relationship between PHLPP2 Akt and insulin signaling highlights its important role in maintaining normal metabolic control and preventing disease progression in these contexts.
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Specifications

Form

Liquid

Additional notes

ab125556 is >95% pure.

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General info

Function

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed : 20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed : 21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed : 24530606).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed : 20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed : 21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed : 24530606).
See full target information PHLPP2
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Complementary Products

Primary Antibodies

AB153918

Anti-PHLPP2 antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-PHLPP2 antibody - N-terminal (AB153918)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Secondary Antibodies

AB150191

Rabbit Anti-Human IgM mu chain (Alexa Fluor® 647)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Human IgM mu chain (Alexa Fluor® 647) (AB150191)

  • 0
  • 0
Secondary Antibodies

AB99817

Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP)

ELISA - Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (AB99817)

  • 0
  • 0

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