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AB160113

Recombinant Human Phosphatidic acid phosphatase type 2B protein (GST tag N-Terminus)

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Recombinant Human Phosphatidic acid phosphatase type 2B protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 311 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

LPP3, PPAP2B, PLPP3, Phospholipid phosphatase 3, Lipid phosphate phosphohydrolase 3, PAP2-beta, Phosphatidate phosphohydrolase type 2b, Phosphatidic acid phosphatase 2b, Vascular endothelial growth factor and type I collagen-inducible protein, PAP-2b, PAP2b, VCIP

1 Images
SDS-PAGE - Recombinant Human Phosphatidic acid phosphatase type 2B protein (GST tag N-Terminus) (AB160113)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Phosphatidic acid phosphatase type 2B protein (GST tag N-Terminus) (AB160113)

ab160113 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

O14495

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Proteins & Peptides

AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

  • 0
  • 0
Proteins & Peptides

AB226446

Recombinant Human ASAH1 protein (His tag)

SDS-PAGE - Recombinant Human ASAH1 protein (His tag) (AB226446)

  • 0
  • 0
Primary Antibodies

AB203216

Anti-Phosphatidic acid phosphatase type 2B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphatidic acid phosphatase type 2B antibody (AB203216)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MQNYKYDKAIVPESKNGGSPALNNNPRRSGSKRVLLICLDLFCLFMAGLPFLIIETSTIKPYHRGFYCNDESIKYPLKTGETINDAVLCAVGIVIAILAIITGEFYRIYYLKKSRSTIQNPYVAALYKQVGCFLFGCAISQSFTDIAKVSIGRLRPHFLSVCNPDFSQINCSEGYIQNYRCRGDDSKVQEARKSFFSGHASFSMYTMLYLVLYLQARFTWRGARLLRPLLQFTLIMMAFYTGLSRVSDHKHHPSDVLAGFAQGALVACCIVFFVSDLFKTKMTLSLPAPAIRKEILSPVDIIDRNNHHNMM","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":311,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O14495","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphatidic acid phosphatase type 2B also known as PAP2B or Lipin-2 plays an important role in lipid metabolism by converting phosphatidic acid to diacylglycerol. This enzyme has a molecular mass of about 47 kDa. It is mainly expressed in liver brain and adipose tissues playing a significant role in regulating lipid levels within these areas. Its activity affects the production and breakdown of fats indicating its importance in metabolic processes.
Biological function summary

This enzyme mediates lipid homeostasis and energy metabolism which are critical for cellular functions. It does not form part of a complex but works by reducing phosphatidic acid levels influencing the signaling pathways that control cell growth and differentiation. By managing lipid synthesis and degradation it helps maintain an equilibrium in lipid-related signaling processes. The conversion it catalyzes influences membrane dynamics and organelle formation.

Pathways

The enzyme actively participates in the glycerolipid biosynthesis and insulin signaling pathways. In the glycerolipid biosynthesis pathway it interacts with related enzymes like acyl-CoA synthetase and diacylglycerol acyltransferase to regulate fatty acid processing. Its role in the insulin signaling pathway affects glucose uptake by modulating lipid signals that dictate metabolic responses to insulin aligning with other vital metabolic proteins like AMPK.

Abnormal levels or function of phosphatidic acid phosphatase type 2B have links to metabolic disorders such as obesity and type 2 diabetes. This enzyme also relates to familial partial lipodystrophy which involves abnormal fat deposition in the body. Its connection to these conditions involves proteins such as perilipin which regulates lipid storage and adiponectin which is involved in insulin sensitivity further demonstrating its significance in lipid and glucose metabolism.
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Specifications

Form

Liquid

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General info

Function

Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P (PubMed : 27694435, PubMed : 9607309, PubMed : 9705349). Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound (PubMed : 9607309). Has both an extracellular and an intracellular phosphatase activity, allowing the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes (PubMed : 23591818, PubMed : 27694435, PubMed : 9607309). Through the dephosphorylation of extracellular sphingosine-1-phosphate and the regulation of its extra- and intracellular availability, plays a role in vascular homeostasis, regulating endothelial cell migration, adhesion, survival, proliferation and the production of pro-inflammatory cytokines (PubMed : 27694435). By maintaining the appropriate levels of this lipid in the cerebellum, also ensure its proper development and function (By similarity). Through its intracellular lipid phosphatase activity may act in early compartments of the secretory pathway, regulating the formation of Golgi to endoplasmic reticulum retrograde transport carriers (PubMed : 23591818).. Independently of this phosphatase activity may also function in the Wnt signaling pathway and the stabilization of beta-catenin/CTNNB1, thereby regulating cell proliferation, migration and differentiation in angiogenesis or yet in tumor growth (PubMed : 20123964, PubMed : 21569306). Also plays a role in integrin-mediated cell-cell adhesion in angiogenesis (PubMed : 12660161, PubMed : 16099422).

Sequence similarities

Belongs to the PA-phosphatase related phosphoesterase family.

Post-translational modifications

N-glycosylated (PubMed:9705349). Contains high-mannose oligosaccharides (PubMed:9705349).

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Product protocols

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Target data

Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P (PubMed : 27694435, PubMed : 9607309, PubMed : 9705349). Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound (PubMed : 9607309). Has both an extracellular and an intracellular phosphatase activity, allowing the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes (PubMed : 23591818, PubMed : 27694435, PubMed : 9607309). Through the dephosphorylation of extracellular sphingosine-1-phosphate and the regulation of its extra- and intracellular availability, plays a role in vascular homeostasis, regulating endothelial cell migration, adhesion, survival, proliferation and the production of pro-inflammatory cytokines (PubMed : 27694435). By maintaining the appropriate levels of this lipid in the cerebellum, also ensure its proper development and function (By similarity). Through its intracellular lipid phosphatase activity may act in early compartments of the secretory pathway, regulating the formation of Golgi to endoplasmic reticulum retrograde transport carriers (PubMed : 23591818).. Independently of this phosphatase activity may also function in the Wnt signaling pathway and the stabilization of beta-catenin/CTNNB1, thereby regulating cell proliferation, migration and differentiation in angiogenesis or yet in tumor growth (PubMed : 20123964, PubMed : 21569306). Also plays a role in integrin-mediated cell-cell adhesion in angiogenesis (PubMed : 12660161, PubMed : 16099422).
See full target information PLPP3
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