Recombinant Human PHOSPHO2 protein is a Human Full Length protein, in the 1 to 241 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M K I L L V F D F D N T I I D D N S D T W I V Q C A P N K K L P I E L R D S Y R K G F W T E F M G R V F K Y L G D K G V R E H E M K R A V T S L P F T P G M V E L F N F I R K N K D K F D C I I I S D S N S V F I D W V L E A A S F H D I F D K V F T N P A A F N S N G H L T V E N Y H T H S C N R C P K N L C K K V V L I E F V D K Q L Q Q G V N Y T Q I V Y I G D G G N D V C P V T F L K N D D V A M P R K G Y T L Q K T L S R M S Q N L E P M E Y S V V V W S S G V D I I S H L Q F L I K D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate.
Alternative names
Pyridoxal phosphate phosphatase PHOSPHO2, PHOSPHO2
Recommended products
Recombinant Human PHOSPHO2 protein is a Human Full Length protein, in the 1 to 241 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q8TCD6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M K I L L V F D F D N T I I D D N S D T W I V Q C A P N K K L P I E L R D S Y R K G F W T E F M G R V F K Y L G D K G V R E H E M K R A V T S L P F T P G M V E L F N F I R K N K D K F D C I I I S D S N S V F I D W V L E A A S F H D I F D K V F T N P A A F N S N G H L T V E N Y H T H S C N R C P K N L C K K V V L I E F V D K Q L Q Q G V N Y T Q I V Y I G D G G N D V C P V T F L K N D D V A M P R K G Y T L Q K T L S R M S Q N L E P M E Y S V V V W S S G V D I I S H L Q F L I K D
- Accession
- Q8TCD6
- Protein length
- Full Length
- Predicted molecular weight
- 30.3 kDa
- Amino acids
- 1 to 241
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab116206 was purified using conventional chromatography techniques.
General info
- Function
Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate.
- Sequence similarities
Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PHOSPHO2 also known as Phosphatase Orphan 2 is a protein that functions mechanically as a phosphatase involved in removing phosphate groups from substrates. This protein has an approximate mass of 31 kDa. PHOSPHO2 is expressed in various tissues including the liver and kidney where it acts in regulating phosphorylation states important for cellular function.
- Biological function summary
The activity of PHOSPHO2 significantly impacts several cellular processes. It is not known to be part of any substantial complexes. Its enzymatic activity regulates the phosphorylation of proteins this is important for signal transduction and cellular communication. By modulating phosphoproteins PHOSPHO2 ensures balanced cellular activities like response to environmental stimuli.
- Pathways
PHOSPHO2 participates in or influences the phosphate metabolism pathway and plays a role in regulatory networks that control signaling pathways. It interacts with other proteins such as phosphoproteins in these pathways. While further research is needed to elucidate its exact partners fully PHOSPHO2's function in phosphorylation suggests general interactions with a variety of signaling proteins within these biological pathways.
- Associated diseases and disorders
There is evidence suggesting a link between PHOSPHO2 activity and metabolic disorders like diabetes. Abnormal regulation of phosphorylation can lead to improper signal transduction contributing to disease pathogenesis. PHOSPHO2 also shares a functional relationship with other phosphatases like PPM1A in these conditions which collectively affects metabolic homeostasis. Understanding these connections may help in developing therapeutic strategies targeting metabolic dysfunctions.
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1 product image
SDS-PAGE - Recombinant Human PHOSPHO2 protein (ab116206)
15% SDS-PAGE analysis of 3 µg ab116206.
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