JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB196098

Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (GST tag N-Term, Tag Free)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (GST tag N-Term, Tag Free) is a Human Full Length protein, in the 1 to 1068 aa range, expressed in Baculovirus infected Sf9 cells, with >52%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphoinositide 3-kinase alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PtdIns-3-kinase subunit p110-alpha, p110alpha, PIK3CA

2 Images
Functional Studies - Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (GST tag N-Term, Tag Free) (AB196098)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (GST tag N-Term, Tag Free) (AB196098)

Kinase assay using ab196098 showing specific activity as 880 pmol/min/μg.

SDS-PAGE - Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (GST tag N-Term, Tag Free) (AB196098)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (GST tag N-Term, Tag Free) (AB196098)

10% SDS-PAGE analysis of 3.2 μg ab196098 with Coomassie staining.

Key facts

Purity

>52% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity: 880 pmol/min/μg.

A unit of ab196098 kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl2, 100 μM ATP, 200 μM PI:PS substrate and ab196098 for 15 min at 30°C.

Accession

P42336

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

style
left-column

Complementary Products

Proteins & Peptides

AB127617

Recombinant human PI3K(p110alpha/p55gamma) protein (Active)

Functional Studies - Recombinant human PI3K(p110alpha/p55gamma) protein (Active) (AB127617)

  • 0
  • 0
Primary Antibodies

AB38449

Anti-AKT (phospho T308) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT (phospho T308) antibody (AB38449)

  • 3
  • 5
Primary Antibodies

AB278545

Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

20ul selling size|Recombinant

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Complex of N-terminal GST-tagged recombinant full length Human PI 3 Kinase p110 alpha and recombinant full length Human PI 3 kinase p85 alpha (no tag) coexpressed in a Baculovirus infected Sf9 cell expression system. MWt 235 kDa (complex).
style
left-column

Sequence info

[{"sequence":"MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMDCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN","proteinLength":"Full Length","predictedMolecularWeight":"151 kDa","actualMolecularWeight":null,"aminoAcidEnd":1068,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P42336","tags":[{"tag":"GST","terminus":"N-Terminus"}]},{"sequence":"MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPGSSKTEADVEQQALTLPDLAEQFAPPDIAPPLLIKLVEAIEKKGLECSTLYRTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPAAVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNERQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR","proteinLength":"Full Length","predictedMolecularWeight":"84 kDa","actualMolecularWeight":null,"aminoAcidEnd":724,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P27986","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI 3-Kinase p110 alpha also known as PIK3CA works as a catalytic subunit of the phosphoinositide 3-kinase class I enzyme. It measures about 110 kDa in mass and binds to the regulatory subunit PI 3-kinase p85 alpha. This complex is primarily expressed in various tissues such as the liver lungs and pancreatic tissue. PI 3-kinase p85 alpha also referred to as PIK3R1 supports stabilization and regulation of the catalytic unit's activity forming an integral part of the enzyme's function.
Biological function summary

This enzyme complex functions in signal transduction pathways converting phosphatidylinositol (45)-bisphosphate to phosphatidylinositol (345)-trisphosphate an important second messenger in cells. This complex influences cellular processes such as growth proliferation and survival. The interaction between p110 alpha and p85 alpha ensures accurate enzyme function contributing to vital signaling outcomes. They commonly function within a complex ensuring the cell responds accurately to external stimuli through these pathways.

Pathways

PI 3-kinase p110 alpha plays a significant role in the PI3K/AKT/mTOR pathway a major pathway regulating cell growth and survival. It works closely with AKT and mTOR proteins to transmit signals that control these biological processes. Another important pathway involving this protein is the insulin signaling pathway which helps in glucose metabolism and energy management. These pathways integrate signals from growth factors and hormones influencing diverse cellular activities.

PI 3-kinase p110 alpha has a well-established connection with various cancers including breast and colorectal cancers. Mutations in the PIK3CA gene which encodes this protein often lead to unregulated activation and are associated with tumorigenesis. Furthermore the dysregulation of this enzyme links to metabolic disorders such as insulin resistance and type 2 diabetes. These conditions illustrate how PI 3-kinase p110 alpha dysregulation can impact associated proteins like AKT and influence disease progression.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 15135396, PubMed : 23936502, PubMed : 28676499). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396, PubMed : 28676499). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed : 23936502, PubMed : 28676499). Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).

Sequence similarities

Belongs to the PI3/PI4-kinase family.

style
left-column

Product protocols

style
left-column

Target data

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 15135396, PubMed : 23936502, PubMed : 28676499). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396, PubMed : 28676499). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed : 23936502, PubMed : 28676499). Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).
See full target information PIK3CA
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com