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AB60892

Recombinant human PI 3 Kinase p110 delta + PI 3 Kinase p85 alpha protein (GST tag N-Term, Tag Free)

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Recombinant human PI 3 Kinase p110 delta + PI 3 Kinase p85 alpha protein (GST tag N-Term, Tag Free) is a Human Full Length protein, in the 1 to 1044 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PI3-kinase subunit delta, PI3K-delta, PI3Kdelta, PtdIns-3-kinase subunit delta, PtdIns-3-kinase subunit p110-delta, p110delta, PIK3CD, GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha

1 Images
SDS-PAGE - Recombinant human PI 3 Kinase p110 delta + PI 3 Kinase p85 alpha protein (GST tag N-Term, Tag Free) (AB60892)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PI 3 Kinase p110 delta + PI 3 Kinase p85 alpha protein (GST tag N-Term, Tag Free) (AB60892)

Lane 1 : 3μg of ab60892.

Lane 2 : Protein marker.

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Active

Accession

O00329

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Proteins & Peptides

AB84769

Recombinant Human PI 3 Kinase p85 alpha protein

Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (AB84769)

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Proteins & Peptides

AB127617

Recombinant human PI3K(p110alpha/p55gamma) protein (Active)

Functional Studies - Recombinant human PI3K(p110alpha/p55gamma) protein (Active) (AB127617)

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Primary Antibodies

AB109006

Anti-PI 3 Kinase p110 delta antibody [EPR386]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [EPR386] (AB109006)

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Primary Antibodies

AB278545

Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

20ul selling size|Recombinant

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Full length p110 Delta - Tagged. Full length p85 - NOT TAGGED.
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1044,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O00329","tags":[{"tag":"GST","terminus":"N-Terminus"}]},{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":724,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P27986","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI 3 Kinase p110 delta together with PI 3 Kinase p85 alpha forms a vital component of the phosphoinositide 3-kinase (PI3K) pathway. PI 3 Kinase p110 delta also known as PI3Kδ has a molecular mass of approximately 120 kDa while PI 3 Kinase p85 alpha also referred to as PI3Kα weighs about 83 kDa. These proteins come together to form a functional heterodimer. PI3Kδ is expressed mostly in leukocytes while PI3Kα shows a broader expression pattern and appears in many cell types including fibroblasts and vascular smooth muscle cells.
Biological function summary

These PI 3 Kinases play important roles in cell signaling. They form a heterodimeric complex that catalyzes the phosphorylation of phosphatidylinositol (45)-bisphosphate (PIP2) to phosphatidylinositol (345)-trisphosphate (PIP3). This modification acts as a signal to promote cell growth proliferation and survival. PI3Kδ is especially important in immune cells where it regulates various functions such as B cell and T cell activation development and migration. PI3Kα on the other hand is more involved in growth factor-related signaling contributing to the growth and survival signals in various tissues.

Pathways

PI3Kδ and PI3Kα participate significantly in the PI3K/AKT/mTOR signaling pathway which is pivotal in cell growth and survival. In this pathway PIP3 generation by PI3Ks leads to the activation of AKT also known as protein kinase B. This phosphorylates several downstream targets including mTOR (mechanistic target of rapamycin) to enhance growth and survival signals. PI3Kα associates closely with receptor tyrosine kinases (RTKs) like the epidermal growth factor receptor while PI3Kδ connects primarily with immune receptor signaling.

Aberrant activation of PI3Kδ and PI3Kα links to various cancers and immune diseases. PI3Kδ mutations often present in chronic lymphocytic leukemia (CLL) and certain lymphomas where it supports cancer cell survival and proliferation. In rheumatoid arthritis dysregulation of PI3Kδ influences immune responses leading to inflammation and joint damage. PI3Kα mutations are frequently detected in breast cancer fueling cell division and tumor growth. Both proteins link to AKT and mTOR which are key targets for therapeutic intervention in these conditions.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 9235916). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Plays a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Plays important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function. Isoform 2 may be involved in stabilizing total RAS levels, resulting in increased ERK phosphorylation and increased PI3K activity.

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Post-translational modifications

Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.

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Product protocols

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Target data

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 9235916). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Plays a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Plays important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function. Isoform 2 may be involved in stabilizing total RAS levels, resulting in increased ERK phosphorylation and increased PI3K activity.
See full target information PIK3CD

Additional targets

PIK3R1
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