Recombinant human PI 3 Kinase p85 alpha + PI 3 Kinase catalytic subunit alpha/PIK3CA (E542K) protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396, PubMed:23936502, PubMed:28676499). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396, PubMed:28676499). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed:23936502, PubMed:28676499). Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).
Additional Targets
PIK3R1, PIK3R1
Alternative names
PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphoinositide 3-kinase alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PtdIns-3-kinase subunit p110-alpha, p110alpha, PIK3CA
Recommended products
Recombinant human PI 3 Kinase p85 alpha + PI 3 Kinase catalytic subunit alpha/PIK3CA (E542K) protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268851 was 810 nmol/min/mg in a kinase assay using a PI(4,5)P2:PS mixture as substrate.
- Accession
- P42336-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence 1
- Accession
- P27986
- Protein length
- Full Length
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 2
- Accession
- P42336
- Protein length
- Full Length
- Amino acids
- 1 to 1068
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396, PubMed:23936502, PubMed:28676499). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396, PubMed:28676499). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed:23936502, PubMed:28676499). Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).
- Sequence similarities
Belongs to the PI3/PI4-kinase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Subunits co-expressed.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PI 3 Kinase p85 alpha also known as PIK3R1 and PI 3 Kinase catalytic subunit alpha (PIK3CA) functions as an important component in cellular signal transduction. These proteins form a Class IA PI 3-kinase complex where p85 alpha serves as the regulatory subunit and p110 alpha acts as the catalytic subunit. PIK3CA the catalytic subunit weighs approximately 124 kDa. These proteins are expressed in various tissues with high expression in adipose tissue liver and the brain playing a significant role in mediating cell growth proliferation and metabolism.
- Biological function summary
These proteins operate as part of a lipid kinase complex. They phosphorylate the inositol ring of phosphoinositides at the 3-position important in the generation of lipid second messengers. The active PI 3-kinase complex primarily phosphorylates phosphatidylinositol (PI) phosphatidylinositol 45-bisphosphate (PI(45)P2) creating phosphatidylinositol 345-trisphosphate (PI(345)P3) which then propagates intracellular signaling cascades. This activity regulates various cellular processes including survival and migration.
- Pathways
PI 3 Kinase p85 alpha and PIK3CA are integral to the PI3K/AKT/mTOR signaling pathway. This pathway plays a pivotal role in regulating cell cycle and is intricately involved in pathways that control cellular quiescence proliferation cancer and longevity. The pathway's components such as AKT and mTOR are critical effectors of PI3K signaling. Additionally the proteins interrelate with other pathways through interactions with proteins like PTEN which acts as a negative regulator by dephosphorylating PI(345)P3.
- Associated diseases and disorders
These components have significant associations with oncogenic processes due to mutations in PIK3CA that are frequently observed in various cancers including breast and colorectal cancer. Such mutations lead to constitutive activation of the PI3K/AKT pathway promoting tumorigenesis. The proteins also link to insulin resistance-related disorders as enhanced PI3K activity affects metabolic signaling. Disorders like these connect through interactions with proteins such as IRS-1 which becomes dysregulated in the context of these diseases further highlighting their implication in aberrant signaling pathways.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Recombinant human PI 3 Kinase p85 alpha + PI 3 Kinase catalytic subunit alpha/PIK3CA (E542K) protein (ab268851)
The specific activity of ab268851 was 810 nmol/min/mg in a kinase assay using a PI(4,5)P2:PS mixture as substrate.
SDS-PAGE - Recombinant human PI 3 Kinase p85 alpha + PI 3 Kinase catalytic subunit alpha/PIK3CA (E542K) protein (ab268851)
SDS-PAGE analysis of ab268851.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com