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Recombinant Human PI 3 Kinase p85 alpha protein is a Human Full Length protein, in the 1 to 724 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB.

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Images

Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (AB84769), expandable thumbnail
  • Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (AB84769), expandable thumbnail

Key facts

Purity
>95% Densitometry
Expression system
Baculovirus infected Sf9 cells
Tags
His tag N-Terminus
Applications
SDS-PAGE, WB
Biologically active
No

Amino acid sequence

M S A E G Y Q Y R A L Y D Y K K E R E E D I D L H L G D I L T V N K G S L V A L G F S D G Q E A R P E E I G W L N G Y N E T T G E R G D F P G T Y V E Y I G R K K I S P P T P K P R P P R P L P V A P G S S K T E A D V E Q Q A L T L P D L A E Q F A P P D I A P P L L I K L V E A I E K K G L E C S T L Y R T Q S S S N L A E L R Q L L D C D T P S V D L E M I D V H V L A D A F K R Y L L D L P N P V I P A A V Y S E M I S L A P E V Q S S E E Y I Q L L K K L I R S P S I P H Q Y W L T L Q Y L L K H F F K L S Q T S S K N L L N A R V L S E I F S P M L F R F S A A S S D N T E N L I K V I E I L I S T E W N E R Q P A P A L P P K P P K P T T V A N N G M N N N M S L Q D A E W Y W G D I S R E E V N E K L R D T A D G T F L V R D A S T K M H G D Y T L T L R K G G N N K L I K I F H R D G K Y G F S D P L T F S S V V E L I N H Y R N E S L A Q Y N P K L D V K L L Y P V S K Y Q Q D Q V V K E D N I E A V G K K L H E Y N T Q F Q E K S R E Y D R L Y E E Y T R T S Q E I Q M K R T A I E A F N E T I K I F E E Q C Q T Q E R Y S K E Y I E K F K R E G N E K E I Q R I M H N Y D K L K S R I S E I I D S R R R L E E D L K K Q A A E Y R E I D K R M N S I K P D L I Q L R K T R D Q Y L M W L T Q K G V R Q K K L N E W L G N E N T E D Q Y S L V E D D E D L P H H D E K T W N V G S S N R N K A E N L L R G K R D G T F L V R E S S K Q G C Y A C S V V V D G E V K H C V I N K T A T G Y G F A E P Y N L Y S S L K E L V L H Y Q H T S L V Q H N D S L N V T L A Y P V Y A Q Q R R

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

ab84769 can be used as a WB positive control in conjunction with ab71925.

Target data

Function

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

Alternative names

Recommended products

Recombinant Human PI 3 Kinase p85 alpha protein is a Human Full Length protein, in the 1 to 724 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB.

Key facts

Purity
>95% Densitometry
Expression system
Baculovirus infected Sf9 cells
Applications
SDS-PAGE, WB
Accession
P27986-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

Sequence info

Amino acid sequence

M S A E G Y Q Y R A L Y D Y K K E R E E D I D L H L G D I L T V N K G S L V A L G F S D G Q E A R P E E I G W L N G Y N E T T G E R G D F P G T Y V E Y I G R K K I S P P T P K P R P P R P L P V A P G S S K T E A D V E Q Q A L T L P D L A E Q F A P P D I A P P L L I K L V E A I E K K G L E C S T L Y R T Q S S S N L A E L R Q L L D C D T P S V D L E M I D V H V L A D A F K R Y L L D L P N P V I P A A V Y S E M I S L A P E V Q S S E E Y I Q L L K K L I R S P S I P H Q Y W L T L Q Y L L K H F F K L S Q T S S K N L L N A R V L S E I F S P M L F R F S A A S S D N T E N L I K V I E I L I S T E W N E R Q P A P A L P P K P P K P T T V A N N G M N N N M S L Q D A E W Y W G D I S R E E V N E K L R D T A D G T F L V R D A S T K M H G D Y T L T L R K G G N N K L I K I F H R D G K Y G F S D P L T F S S V V E L I N H Y R N E S L A Q Y N P K L D V K L L Y P V S K Y Q Q D Q V V K E D N I E A V G K K L H E Y N T Q F Q E K S R E Y D R L Y E E Y T R T S Q E I Q M K R T A I E A F N E T I K I F E E Q C Q T Q E R Y S K E Y I E K F K R E G N E K E I Q R I M H N Y D K L K S R I S E I I D S R R R L E E D L K K Q A A E Y R E I D K R M N S I K P D L I Q L R K T R D Q Y L M W L T Q K G V R Q K K L N E W L G N E N T E D Q Y S L V E D D E D L P H H D E K T W N V G S S N R N K A E N L L R G K R D G T F L V R E S S K Q G C Y A C S V V V D G E V K H C V I N K T A T G Y G F A E P Y N L Y S S L K E L V L H Y Q H T S L V Q H N D S L N V T L A Y P V Y A Q Q R R
Accession
P27986
Protein length
Full Length
Amino acids
1 to 724
Nature
Recombinant
Tags
His tag N-Terminus

Specifications

Form
Liquid
Additional notes

Affinity purified.

General info

Function

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

Sequence similarities

Belongs to the PI3K p85 subunit family.

Post-translational modifications

Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PI 3 Kinase p85 alpha also known as PI3KR1 or phospho PI3K is a regulatory subunit of phosphoinositide 3-kinase (PI3K) that plays a fundamental role in various signaling pathways. The p85 protein has a molecular weight of approximately 85 kDa. This protein is widely expressed in many cell types facilitating essential cellular functions by forming a heterodimer with the class I PI3K catalytic subunit often implicated in regulating lipid signaling pathways.

Biological function summary

P85 alpha modulates intracellular signaling by interacting with other proteins in a complex formation with catalytic subunit p110. This interaction enables the initiation of downstream signaling cascades controlling cell growth proliferation and survival. PI 3 kinase activity mediated by p85 alpha is integral to cellular homeostasis and metabolism helping to execute cellular responses to a variety of growth factors and hormones.

Pathways

PI 3 Kinase p85 alpha integrates into two principal signaling networks: the PI3K-Akt and mTOR pathways. These pathways are instrumental for regulating anabolic processes and cell survival. The p85 form of PI3K interacts with additional proteins such as Akt and mTOR coordinating cellular energy balance promoting protein synthesis and inhibiting apoptosis. Through these interactions the protein ensures that cells respond accurately to nutritional and stress signals.

Associated diseases and disorders

Alterations in PI 3 Kinase p85 alpha function have been linked to cancer and type 2 diabetes. Aberrant signaling involving PI3K-Akt-mTOR pathways due to mutations in PI3KR1 can contribute to tumor progression and chemoresistance. Moreover dysfunction of this pathway affects insulin signaling connecting p85 alpha to diabetic pathologies. Proteins such as IRS-1 (insulin receptor substrate 1) act alongside p85 alpha in mediating these disease-associated processes emphasizing its importance in maintaining physiological balance.

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2 product images

  • Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (ab84769), expandable thumbnail

    Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (ab84769)

    All lanes: Anti-PI 3 Kinase p85 alpha antibody (ab71925) at 1 µg/mL

    Lane 1: Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (ab84769) at 0.1 µg

    Lane 2: Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (ab84769) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10s

  • Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (ab84769), expandable thumbnail

    Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (ab84769)

    SDS-PAGE showing ab84769 at approximately 86kDa.

    All lanes: Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (ab84769)

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