Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 alpha protein (Active) is a Human Full Length protein, in the 1 to 1070 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also a protein kinase activity showing autophosphorylation (PubMed:12502714).
Additional Targets
PIK3R1
Alternative names
PIK3C1, PIK3CB, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Serine/threonine protein kinase PIK3CB, PtdIns-3-kinase subunit p110-beta, p110beta
Recommended products
Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 alpha protein (Active) is a Human Full Length protein, in the 1 to 1070 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268856 was 540 nmol/min/mg in a kinase assay using a PI(4,5)P2:PS mixture as substrate.
- Accession
- P42338-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence 1
- Accession
- P42338
- Protein length
- Full Length
- Amino acids
- 1 to 1070
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 2
- Accession
- P27986
- Protein length
- Full Length
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also a protein kinase activity showing autophosphorylation (PubMed:12502714).
- Sequence similarities
Belongs to the PI3/PI4-kinase family.
- Post-translational modifications
Autophosphorylation at Ser-1070 negatively regulates the phosphatidylinositol-4,5-bisphosphate 3-kinase activity.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Subunits co-expressed.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PI3 Kinase p110 beta also known as PIK3CB functions as a catalytic subunit of phosphoinositide 3-kinase (PI3K). It has a molecular mass of approximately 122 kDa. PI3K p110 beta partners with the regulatory subunit p85 alpha which stabilizes and modulates its activity. The protein is expressed in many tissues with notable presence in the heart liver and skeletal muscles. This expression suggests its involvement in a range of cellular processes that require tight regulation.
- Biological function summary
PI3K p110 beta contributes to intracellular signaling pathways that manage cell growth survival and metabolism. It forms a complex with the full-length PI3K protein alongside p85 alpha enhancing its role in signal transduction. The function of the full length of PI3 kinase which includes both p110 beta and p85 alpha subunits impacts processes like cellular proliferation and apoptosis. The interplay between these subunits within the complex influences the intensity and duration of the signaling cascade.
- Pathways
PI3K p110 beta holds an important position in the PI3K/AKT/mTOR pathway which is essential for mediating signals from growth factors like insulin. It closely interacts with other proteins such as phosphatidylinositol (PI) and protein kinase B (AKT). The PI3K pathway including the p110 beta and p85 protein components communicates signals that regulate cell cycle progression and prevent apoptosis showcasing an important connection between nutrient availability and cellular response.
- Associated diseases and disorders
PI3K p110 beta has been implicated in cancer and type 2 diabetes due to its influence on cell growth and glucose metabolism. Aberrations in the enzyme's activity therefore in its related pathway involving PI protein interactions can lead to excessive cell division as seen in cancers. The regulatory subunit p85 alpha also plays a role as its dysfunction may disrupt the balance between survival and apoptotic signals further promoting oncogenesis and glucose metabolic disorders.
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2 product images
Functional Studies - Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 alpha protein (Active) (ab268856)
The specific activity of ab268856 was 540 nmol/min/mg in a kinase assay using a PI(4,5)P2:PS mixture as substrate.
SDS-PAGE - Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 alpha protein (Active) (ab268856)
SDS-PAGE analysis of ab268856.
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