JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB268857

Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 beta protein (Active)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 beta protein (Active) is a Human Full Length protein, in the 1 to 728 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PIK3C1, PIK3CB, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Serine/threonine protein kinase PIK3CB, PtdIns-3-kinase subunit p110-beta, p110beta

2 Images
Functional Studies - Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 beta protein (Active) (AB268857)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 beta protein (Active) (AB268857)

The specific activity of ab268857 was 360 nmol/min/mg in a kinase assay using a PI(4,5)P2 : PS mixture as substrate.

SDS-PAGE - Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 beta protein (Active) (AB268857)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 beta protein (Active) (AB268857)

SDS-PAGE analysis of asb268857.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab268857 was 360 nmol/min/mg in a kinase assay using a PI(4,5)P2:PS mixture as substrate.

Accession

P42338

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Subunits co-expressed.
style
left-column

Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":728,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O00459","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1070,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P42338","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI3 Kinase p110 beta also known as p110β and PI 3 Kinase p85 beta form part of a heterodimer complex where the p110β serves as the catalytic unit and p85β as the regulatory subunit. The molecular mass of p110β is approximately 122 kDa. These proteins are components of the phosphoinositide 3-kinase (PI3K) pathways and their expression occurs broadly across different tissues in the human body. Both proteins have a strong presence in tissues such as the liver and fat wherein they modulate various cellular functions.
Biological function summary

These subunits interact closely to regulate important cellular processes including cell growth metabolism and survival signals. The heterodimer acts as a vital signaling entity that helps mediate the response to extracellular signals. Through their interaction with regulatory and catalytic subunits they modulate the lipid kinase activities contributing to cellular proliferation and glucose uptake. The complex nature of their interactions ensures precise regulation of downstream signaling pathways.

Pathways

The PI3 Kinase pathway is central to many intracellular signaling routes notably the AKT signaling pathway. This association influences processes like cell cycle progression and apoptosis control. Apart from AKT they also connect with related proteins such as PTEN an important negative regulator that helps maintain balance within the pathway. The engagement of p110β and p85β in these pathways demonstrates their roles in translating external signals into diverse cellular responses.

Altered activity of PI3 Kinase p110 beta and PI 3 Kinase p85 beta can lead to various conditions including cancer and diabetes. These proteins often exhibit dysregulated signaling in tumorigenesis where their interactions with oncogenic proteins like PTEN become disrupted. Similarly in diabetes impaired signaling pathways involving these subunits can affect glucose metabolism. The sensitive regulation of these proteins holds significant importance in understanding their roles in disease mechanisms.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also a protein kinase activity showing autophosphorylation (PubMed : 12502714).

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Post-translational modifications

Autophosphorylation at Ser-1070 negatively regulates the phosphatidylinositol-4,5-bisphosphate 3-kinase activity.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also a protein kinase activity showing autophosphorylation (PubMed : 12502714).
See full target information PIK3CB

Additional targets

PIK3R2
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB151549

Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)

  • 5
  • 2
Proteins & Peptides

AB84769

Recombinant Human PI 3 Kinase p85 alpha protein

Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (AB84769)

  • 0
  • 0
Proteins & Peptides

AB127617

Recombinant human PI3K(p110alpha/p55gamma) protein (Active)

Functional Studies - Recombinant human PI3K(p110alpha/p55gamma) protein (Active) (AB127617)

  • 0
  • 0
Biochemicals

AB120243

LY 294002, PI3-kinase inhibitor

Western blot - LY 294002, PI3-kinase inhibitor (AB120243)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com