Recombinant human PI3K (p110 delta/p85 alpha) protein (Active) is a Human Full Length protein, in the 2 to 724 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, WB, FuncS.
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- Stem cells international 2022:56095012022MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinmin Cai,Liufang ShengPubMed 36277041
- Frontiers in neurology 12:6533412021LncRNA GAS5 as miR-26a-5p Sponge Regulates the PTEN/PI3K/Akt Axis and Affects Extracellular Matrix Synthesis in Degenerative Nucleus Pulposus Cells .Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Tan,Yifang Xie,Ye Yuan,Kai HuPubMed 34413821
- Frontiers in molecular neuroscience 13:952020LncRNA LINC00641 Sponges miR-497-5p to Ameliorate Neural Injury Induced by Anesthesia via Up-Regulating BDNF.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingxia Chen,Jingjia Yan,Wenji Xie,Wenqin Xie,Meijun Li,Yanle YePubMed 32714145
- The Journal of biological chemistry 295:8236-82512020Transforming growth factor β (TGF-β) receptor signaling regulates kinase networks and phosphatidylinositol metabolism during T-cell activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard T Cattley et. al.PubMed 32358062
- Molecular medicine reports 20:1187-11952019Alisol B 23‑acetate inhibits the viability and induces apoptosis of non‑small cell lung cancer cells via PI3K/AKT/mTOR signal pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Liu et. al.PubMed 31173235
- Molecular medicine reports 19:3658-36662019Knockdown of serpin peptidase inhibitor clade C member 1 inhibits the growth of nasopharyngeal carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Xu et. al.PubMed 30896875
- Oncology letters 15:9377-93842018Inhibition of the PI3K/Akt signaling pathway reverses sorafenib-derived chemo-resistance in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Zhang,Qingqing Wang,Jun Liu,Haoqiang CaoPubMed 29928334
- Oncology research 26:869-8782017Overexpression of Long Noncoding RNA PTENP1 Inhibits Cell Proliferation and Migration via Suppression of miR-19b in Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianbiao Shi,Xiaoqiao Tang,Lei SuPubMed 29212574
Key facts
- Purity
- >75% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).
Additional Targets
PIK3CD
Alternative names
GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha
Recommended products
Recombinant human PI3K (p110 delta/p85 alpha) protein (Active) is a Human Full Length protein, in the 2 to 724 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >75% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab125633 was equivalent to 2,514 nmol/min/mg as per activity assay protocol.
- Accession
- P27986-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence 1
- Accession
- P27986
- Protein length
- Full Length
- Predicted molecular weight
- 86 kDa
- Amino acids
- 2 to 724
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 2
- Accession
- O00329
- Protein length
- Full Length
- Predicted molecular weight
- 111 kDa
- Amino acids
- 1 to 1044
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).
- Sequence similarities
Belongs to the PI3K p85 subunit family.
- Post-translational modifications
Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Kinase assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PI3K (p110 delta/p85 alpha) also known as phosphoinositide 3-kinase delta and alpha is composed of two subunits: the catalytic p110 delta and the regulatory p85 alpha. The complex has a molecular mass of about 124 kDa for p110 delta and 83 kDa for p85 alpha. PI3K is mainly expressed in leukocytes where it plays roles in immune responses. Its activity involves phosphorylation of phosphoinositides at the 3-position of the inositol ring acting as an important player in signal transduction.
- Biological function summary
PI3K influences cell growth survival and metabolism by producing lipid second messengers. It partners with other PI3K proteins including p85 PI3K forming heterodimeric complexes. PI3K delta is important in hematopoietic cells promoting cellular responses to stimuli and modulating immune system activity. Dysfunction in these activities can lead to immune system disorders. The dual role of PI3K in both regulating normal cellular processes and contributing to pathologies makes it an attractive therapeutic target.
- Pathways
PI3K is a central component of the PI3K/AKT signaling pathway which is significant in mediating cellular proliferation and survival. This pathway interacts with other signaling molecules such as the mTOR pathway to manage cellular growth and metabolism. PI3K works closely with proteins like PTEN a phosphatase that negatively regulates the pathway and AKT a downstream effector. Understanding these interactions highlights their importance in maintaining cellular homeostasis and exploring therapeutic strategies.
- Associated diseases and disorders
Dysregulation of PI3K activity links to cancer and inflammatory diseases. In cancer mutations or overexpression of PI3K delta can lead to uncontrolled cell proliferation. Targeting PI3K with inhibitors offers potential for cancer treatment. In inflammatory disorders altered PI3K signaling can result in abnormal immune responses suggesting its role in diseases like rheumatoid arthritis. Researchers focus on modulating PI3K activity to develop treatments for these conditions particularly addressing its interactions with proteins like mTOR and PTEN.
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2 product images
Functional Studies - Recombinant human PI3K (p110 delta/p85 alpha) protein (Active) (ab125633)
The specific activity of ab125633 was equivalent to 2,514 nmol/min/mg as per activity assay protocol.
SDS-PAGE - Recombinant human PI3K (p110 delta/p85 alpha) protein (Active) (ab125633)
Purity of ab125633 was determined to be >75% by densiometry.
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