Recombinant human PI3K(p110alpha/p55gamma) protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB, FuncS.
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- Molecular vision 27:725-7332021Ligustrazine protects against chronic hypertensive glaucoma in rats by inhibiting autophagy via the PI3K-Akt/mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Yan Du,Rong Wang,Jian-Liang Li,Huang Luo,Xiao-Yan Xie,Ran Yan,Yue-Ling Jian,Jin-Ying CaiPubMed 35035207
- Experimental and therapeutic medicine 21:3732021MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZengxi Xin,Zhou Tong,Jingyu Tan,Changfu LiuPubMed 33732346
- Bioengineered 12:507-5152021Ligustrazine induces viability, suppresses apoptosis and autophagy of retinal ganglion cells with ischemia/reperfusion injury through the PI3K/Akt/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Yan Du,Rong Wang,Jian-Liang Li,Huang Luo,Xiao-Yan Xie,Ran Yan,Yue-Ling Jian,Jin-Ying CaiPubMed 33522374
- Molecular medicine reports 21:1709-17162020Long non‑coding RNA H19 protects H9c2 cells against hypoxia‑induced injury by activating the PI3K/AKT and ERK/p38 pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinhui Yuan et. al.PubMed 32319634
- Journal of experimental & clinical cancer research 38:4102019UFM1 suppresses invasive activities of gastric cancer cells by attenuating the expres7sion of PDK1 through PI3K/AKT signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian-Xian Lin et. al.PubMed 31533855
- Experimental and therapeutic medicine 18:3543-35512019MicroRNA-21-5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangfu Liu et. al.PubMed 31602231
- Cell proliferation 52:e126512019Long non-coding RNA HOTAIR/microRNA-206 sponge regulates STC2 and further influences cell biological functions in head and neck squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTiancheng Li et. al.PubMed 31297902
- Neuroscience bulletin 35:471-4852019Up-Regulation of Trem2 Inhibits Hippocampal Neuronal Apoptosis and Alleviates Oxidative Stress in Epilepsy via the PI3K/Akt Pathway in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAi-Hua Liu et. al.PubMed 30684126
- Pathology, research and practice 215:115-1202018MicroRNA-17 promotes osteosarcoma cells proliferation and migration and inhibits apoptosis by regulating SASH1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDajiang Wu,Hao Zhang,Fang Ji,Wenbin DingPubMed 30396754
Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
H H H H H H L V P R G S P E F M P P R P S S G E L W G I H L M P P R I L V E C L L P N G M I V T L E C L R E A T L I T I K H E L F K E A R K Y P L H Q L L Q D E S S Y I F V S V T Q E A E R E E F F D E T R R L C D L R L F Q P F L K V I E P V G N R E P K I L N R E I G F A I G M P V C E F D M V K D P E V Q D F R R N I L N V C K E A V D L R D L N S P H S R A M Y V Y P P N V E S S P E L P K H I Y N K L D K G Q I I V V I W V I V S P N N D K Q K Y T L K I N H D C V P E Q V I A E A I R K K T R S M L L S S E Q L K L C V L E Y Q G K Y I L K V C G C D E Y F L E K Y P L S Q Y K Y I R S C I M L G R M P N L M L M A K E S L Y S Q L P M D C F T M P S Y S R R I S T A T P Y M N G E T S T K S L W V I N S A L R I K I L C A T Y V N V N I R D I D K I Y V R T G I Y H G G E P L C D N V N T Q R V P C S N P R W N E W L N Y D I Y I P D L P R A A R L C L S I C S V K G R K G A K E E H C P L A W G N I N L F D Y T D T L V S G K M A L N L W P V P H G L E D L L N P I G V T G S N P N K E T P C L E L E F D W F S S V V K F P D M S V I E E H A N W S V S R E A G F S Y S H A G L S N R L A R D N E L R E N D K E Q L K A I S T R D P L S E I T E Q E K D F L W S H R H Y C V T I P E I L P K L L L S V K W N S R D E V A Q M Y C L V K D W P P I K P E Q A M E L L D C N Y P D P M V R G F A V R C L E K Y L T D D K L S Q Y L I Q L V Q V L K Y E Q Y L D N L L V R F L L K K A L T N Q R I G H F F F W H L K S E M H N K T V S Q R F G L L L E S Y C R A C G M Y L K H L N R Q V E A M E K L I N L T D I L K Q E K K D E T Q K V Q M K F L V E Q M R R P D F M D A L Q G F L S P L N P A H Q L G N L R L E E C R I M S S A K R P L W L N W E N P D I M S E L L F Q N N E I I F K N G D D L R Q D M L T L Q I I R I M E N I W Q N Q G L D L R M L P Y G C L S I G D C V G L I E V V R N S H T I M Q I Q C K G G L K G A L Q F N S H T L H Q W L K D K N K G E I Y D A A I D L F T R S C A G Y C V A T F I L G I G D R H N S N I M V K D D G Q L F H I D F G H F L D H K K K K F G Y K R E R V P F V L T Q D F L I V I S K G A Q E C T K T R E F E R F Q E M C Y K A Y L A I R Q H A N L F I N L F S M M L G S G M P E L Q S F D D I A Y I R K T L A L D K T E Q E A L E Y F M K Q M N D A H H G G W T T K M D W I F H T I K Q H A L N M Y N T V W S M D R D D A D W R E V M M P Y S T E L I F Y I E M D P P A L P P K P P K P M T S A V P N G M K P S S V S L Q D A E W Y W G D I S R E E V N D K L R D M P D G T F L V R D A S T K M Q G D Y T L T L R K G G N N K L I K I Y H R D G K Y G F S D P L T F N S V V E L I N H Y H H E S L A Q Y N P K L D V K L M Y P V S R Y Q Q D Q L V K E D N I D A V G K K L Q E Y H S Q Y Q E K S K E Y D R L Y E E Y T R T S Q E I Q M K R T A I E A F N E T I K I F E E Q C H T Q E Q H S K E Y I E R F R R E G N E K E I E R I M M N Y D K L K S R L G E I H D S K M R L E Q D L K N Q A L D N R E I D K K M N S I K P D L I Q L R K I R D Q H L V W L N H K G V R Q K R L N V W L G I K N E D A D E N Y F I N E E D E N L P H Y D E K T W F V E D I N R V Q A E D L L Y G K P D G A F L I R E S S K K G C Y A C S V V A D G E V K H C V I Y S T A R G Y G F A E P Y N L Y S S L K E L V L H Y Q Q T S L V Q H N D S L N V R L A Y P V H A Q M P S L C R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396, PubMed:23936502, PubMed:28676499). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396, PubMed:28676499). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed:23936502, PubMed:28676499). Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).
Additional Targets
PIK3CA
Alternative names
PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphoinositide 3-kinase alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PtdIns-3-kinase subunit p110-alpha, p110alpha, PIK3CA
Recommended products
Recombinant human PI3K(p110alpha/p55gamma) protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- 420 nmol/min/mg.
- Accession
- P42336-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- H H H H H H L V P R G S P E F M P P R P S S G E L W G I H L M P P R I L V E C L L P N G M I V T L E C L R E A T L I T I K H E L F K E A R K Y P L H Q L L Q D E S S Y I F V S V T Q E A E R E E F F D E T R R L C D L R L F Q P F L K V I E P V G N R E P K I L N R E I G F A I G M P V C E F D M V K D P E V Q D F R R N I L N V C K E A V D L R D L N S P H S R A M Y V Y P P N V E S S P E L P K H I Y N K L D K G Q I I V V I W V I V S P N N D K Q K Y T L K I N H D C V P E Q V I A E A I R K K T R S M L L S S E Q L K L C V L E Y Q G K Y I L K V C G C D E Y F L E K Y P L S Q Y K Y I R S C I M L G R M P N L M L M A K E S L Y S Q L P M D C F T M P S Y S R R I S T A T P Y M N G E T S T K S L W V I N S A L R I K I L C A T Y V N V N I R D I D K I Y V R T G I Y H G G E P L C D N V N T Q R V P C S N P R W N E W L N Y D I Y I P D L P R A A R L C L S I C S V K G R K G A K E E H C P L A W G N I N L F D Y T D T L V S G K M A L N L W P V P H G L E D L L N P I G V T G S N P N K E T P C L E L E F D W F S S V V K F P D M S V I E E H A N W S V S R E A G F S Y S H A G L S N R L A R D N E L R E N D K E Q L K A I S T R D P L S E I T E Q E K D F L W S H R H Y C V T I P E I L P K L L L S V K W N S R D E V A Q M Y C L V K D W P P I K P E Q A M E L L D C N Y P D P M V R G F A V R C L E K Y L T D D K L S Q Y L I Q L V Q V L K Y E Q Y L D N L L V R F L L K K A L T N Q R I G H F F F W H L K S E M H N K T V S Q R F G L L L E S Y C R A C G M Y L K H L N R Q V E A M E K L I N L T D I L K Q E K K D E T Q K V Q M K F L V E Q M R R P D F M D A L Q G F L S P L N P A H Q L G N L R L E E C R I M S S A K R P L W L N W E N P D I M S E L L F Q N N E I I F K N G D D L R Q D M L T L Q I I R I M E N I W Q N Q G L D L R M L P Y G C L S I G D C V G L I E V V R N S H T I M Q I Q C K G G L K G A L Q F N S H T L H Q W L K D K N K G E I Y D A A I D L F T R S C A G Y C V A T F I L G I G D R H N S N I M V K D D G Q L F H I D F G H F L D H K K K K F G Y K R E R V P F V L T Q D F L I V I S K G A Q E C T K T R E F E R F Q E M C Y K A Y L A I R Q H A N L F I N L F S M M L G S G M P E L Q S F D D I A Y I R K T L A L D K T E Q E A L E Y F M K Q M N D A H H G G W T T K M D W I F H T I K Q H A L N M Y N T V W S M D R D D A D W R E V M M P Y S T E L I F Y I E M D P P A L P P K P P K P M T S A V P N G M K P S S V S L Q D A E W Y W G D I S R E E V N D K L R D M P D G T F L V R D A S T K M Q G D Y T L T L R K G G N N K L I K I Y H R D G K Y G F S D P L T F N S V V E L I N H Y H H E S L A Q Y N P K L D V K L M Y P V S R Y Q Q D Q L V K E D N I D A V G K K L Q E Y H S Q Y Q E K S K E Y D R L Y E E Y T R T S Q E I Q M K R T A I E A F N E T I K I F E E Q C H T Q E Q H S K E Y I E R F R R E G N E K E I E R I M M N Y D K L K S R L G E I H D S K M R L E Q D L K N Q A L D N R E I D K K M N S I K P D L I Q L R K I R D Q H L V W L N H K G V R Q K R L N V W L G I K N E D A D E N Y F I N E E D E N L P H Y D E K T W F V E D I N R V Q A E D L L Y G K P D G A F L I R E S S K K G C Y A C S V V A D G E V K H C V I Y S T A R G Y G F A E P Y N L Y S S L K E L V L H Y Q Q T S L V Q H N D S L N V R L A Y P V H A Q M P S L C R
- Accession
- Q92569
- Protein length
- Full Length
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396, PubMed:23936502, PubMed:28676499). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396, PubMed:28676499). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed:23936502, PubMed:28676499). Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).
- Sequence similarities
Belongs to the PI3/PI4-kinase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PI3K p110alpha/p55gamma often referred to as PI3Kα is a member of the phosphoinositide 3-kinases (PI3Ks) family. It plays a significant role in intracellular signal transduction. PI3Kα is a heterodimer composed of a p110 catalytic subunit and a p55 regulatory subunit. The molecular mass of the p110alpha subunit is approximately 124 kDa while the p55gamma regulatory subunit has a mass of around 55 kDa. PI3Kα expresses mainly in tissues with high cellular proliferation rates such as liver heart and muscle indicating its involvement in key biological processes.
- Biological function summary
PI3Kα regulates cellular functions by participating in lipid phosphorylation at the 3-position of the inositol ring producing phosphatidylinositol (345)-trisphosphate (PIP3). This is an important event in recruiting signaling proteins with PH domains to the membrane leading to the activation of kinases like AKT. PI3Kα usually forms part of a complex with other proteins in order to facilitate its functions. This complex arrangement aids in mediating cell growth proliferation survival and metabolic responses making it a central player in cellular physiology.
- Pathways
PI3Kα integrates into the PI3K/AKT/mTOR pathway a critical signaling cascade that controls myriad cellular processes. Within this pathway PI3Kα activates AKT which in turn interacts with downstream effectors like mTOR to regulate cellular metabolism and growth. PI3Kα also connects with proteins like PTEN and PDK1 to maintain a balance between cell survival and apoptosis. Besides this pathway influences glucose homeostasis and can affect various cellular states through intricate signaling networks.
- Associated diseases and disorders
PI3Kα links strongly with cancer and insulin resistance conditions like Type 2 diabetes. Mutations and overexpression of PI3Kα have associations with several cancer types including breast and colorectal cancers by driving unchecked cellular proliferation and survival. In insulin resistance dysfunctional PI3Kα impair signaling through the insulin pathway reducing the responsiveness of cells to insulin and affecting glucose uptake. The interaction of PI3Kα with proteins such as PTEN and AKT plays an important role in these pathological conditions underlining its importance as a target in therapeutic interventions.
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4 product images
Functional Studies - Recombinant human PI3K(p110alpha/p55gamma) protein (Active) (ab127617)
The specific activity of PI3K(p110alpha/p55gamma) (ab127617) was determined to be 357 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human PI3K(p110alpha/p55gamma) protein (Active) (ab127617)
SDS PAGE analysis of ab127617
Functional Studies - Recombinant human PI3K(p110alpha/p55gamma) protein (Active) (ab127617)
The specific activity of ab127617 was found to be 420 nmol/min/mg as per activity assay protocol.
SDS-PAGE - Recombinant human PI3K(p110alpha/p55gamma) protein (Active) (ab127617)
Purity >70% by densitometry.
p110alpha ~111kDa
p55gamma ~61kDa
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