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AB268860

Recombinant human PI4K2A protein (Active)

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Recombinant human PI4K2A protein (Active) is a Human Full Length protein, in the 1 to 479 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI4K2A

2 Images
Functional Studies - Recombinant human PI4K2A protein (Active) (AB268860)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PI4K2A protein (Active) (AB268860)

The specific activity of ab268860 was 1,500 nmol/min/mg in a kinase assay using PI : PS as substrate.

SDS-PAGE - Recombinant human PI4K2A protein (Active) (AB268860)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PI4K2A protein (Active) (AB268860)

SDS-PAGE analysis of ab268860.

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab268860 was 1,500 nmol/min/mg in a kinase assay using PI:PS as substrate.

Accession

Q9BTU6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Proteins & Peptides

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Proteins & Peptides

AB316439

Recombinant Human BCMA Protein Standard (His tag)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MDETSPLVSPERAQPPDYTFPSGSGAHFPQVPGGAVRVAAAAGSGPSPPGSPGHDRERQPLLDRARGAAAQGQTQTVAAQAQALAAQAAAAAHAAQAHRERNEFPEDPEFEAVVRQAELAIERCIFPERIYQGSSGSYFVKDPQGRIIAVFKPKNEEPYGHLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDSSSSRDTDWVVVKEPVIKVAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFVKDLEEDLYELFKKDPGFDRGQFHKQIAVMRGQILNLTQALKDNKSPLHLVQMPPVIVETARSHQRSSSESYTQSFQSRKPFFSWW","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":479,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9BTU6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI4K2A also known as phosphatidylinositol 4-kinase type 2 alpha is an enzyme involved in phospholipid signaling. It catalyzes the phosphorylation of phosphatidylinositol (PI) to generate phosphatidylinositol 4-phosphate (PI4P). This enzyme is part of the PI4K family and typically has a mass of approximately 55 kDa. PI4K2A is primarily expressed in brain tissue but can also be found in other tissues such as heart and liver.
Biological function summary

PI4K2A plays a significant role in cellular processes through its involvement in phosphoinositide turnover. It contributes to the synthesis of membrane phospholipids and helps regulate vesicle trafficking. PI4K2A functions as part of larger protein complexes involved in generating PI4P a precursor for producing phosphatidylinositol 45-bisphosphate (PI(45)P2) which is critical for various signaling pathways.

Pathways

PI4K2A associates with the phosphoinositide signaling pathway and the endocytic pathway. In the phosphoinositide signaling pathway it interacts with other kinases to facilitate the production of PI4P and PI(45)P2 which serve as signaling molecules. Within these pathways PI4K2A collaborates with proteins like PI4KB to regulate membrane dynamics and endocytosis.

PI4K2A has connections to neurodegenerative diseases and some cardiovascular conditions. Abnormal PI4K2A expression or function may disrupt phosphoinositide balance affecting cellular signaling and leading to disease. It relates to the LRRK2 protein in Parkinson’s disease where dysregulated PI4P levels have implications for disease progression. Furthermore in cardiovascular disorders altered PI4K2A activity may impact heart muscle functionality contributing to disease development.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Membrane-bound phosphatidylinositol-4 kinase (PI4-kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).

Sequence similarities

Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.

Post-translational modifications

Palmitoylated by ZDHHC3 and ZDHHC7 in the CCPCC motif. Palmitoylation is cholesterol-dependent, and required for TGN localization.. Ubiquitinated by ITCH; this does not lead to proteasomal degradation.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Membrane-bound phosphatidylinositol-4 kinase (PI4-kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).
See full target information PI4K2A
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