Recombinant human PI4K2B protein (Active) is a Human Full Length protein, in the 1 to 481 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M E D P S E P D R L A S A D G G S P E E E E D G E R E P L L P R I A W A H P R R G A P G S A V R L L D A A G E E G E A G D E E L P L P P G D V G V S R S S S A E L D R S R P A V S V T I G T S E M N A F L D D P E F A D I M L R A E Q A I E V G I F P E R I S Q G S S G S Y F V K D P K R K I I G V F K P K S E E P Y G Q L N P K W T K Y V H K V C C P C C F G R G C L I P N Q G Y L S E A G A Y L V D N K L H L S I V P K T K V V W L V S E T F N Y N A I D R A K S R G K K Y A L E K V P K V G R K F H R I G L P P K I G S F Q L F V E G Y K E A E Y W L R K F E A D P L P E N I R K Q F Q S Q F E R L V I L D Y I I R N T D R G N D N W L V R Y E K Q K C E K E I D H K E S K W I D D E E F L I K I A A I D N G L A F P F K H P D E W R A Y P F H W A W L P Q A K V P F S E E I R N L I L P Y I S D M N F V Q D L C E D L Y E L F K T D K G F D K A T F E S Q M S V M R G Q I L N L T Q A L R D G K S P F Q L V Q I P C V I V E R S Q G G S Q G R I V H L S N S F T Q T V N C R K P F F S S W
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell (PubMed:11923287, PubMed:12324459). This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments (PubMed:11923287, PubMed:12324459). The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3) (PubMed:11923287, PubMed:12324459). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.
Alternative names
Phosphatidylinositol 4-kinase type 2-beta, Phosphatidylinositol 4-kinase type II-beta, PI4KII-BETA, PI4K2B
Recommended products
Recombinant human PI4K2B protein (Active) is a Human Full Length protein, in the 1 to 481 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268861 was 1,573 nmol/min/mg in a kinase assay using PI:PS as substrate.
- Accession
- Q8TCG2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M E D P S E P D R L A S A D G G S P E E E E D G E R E P L L P R I A W A H P R R G A P G S A V R L L D A A G E E G E A G D E E L P L P P G D V G V S R S S S A E L D R S R P A V S V T I G T S E M N A F L D D P E F A D I M L R A E Q A I E V G I F P E R I S Q G S S G S Y F V K D P K R K I I G V F K P K S E E P Y G Q L N P K W T K Y V H K V C C P C C F G R G C L I P N Q G Y L S E A G A Y L V D N K L H L S I V P K T K V V W L V S E T F N Y N A I D R A K S R G K K Y A L E K V P K V G R K F H R I G L P P K I G S F Q L F V E G Y K E A E Y W L R K F E A D P L P E N I R K Q F Q S Q F E R L V I L D Y I I R N T D R G N D N W L V R Y E K Q K C E K E I D H K E S K W I D D E E F L I K I A A I D N G L A F P F K H P D E W R A Y P F H W A W L P Q A K V P F S E E I R N L I L P Y I S D M N F V Q D L C E D L Y E L F K T D K G F D K A T F E S Q M S V M R G Q I L N L T Q A L R D G K S P F Q L V Q I P C V I V E R S Q G G S Q G R I V H L S N S F T Q T V N C R K P F F S S W
- Accession
- Q8TCG2
- Protein length
- Full Length
- Amino acids
- 1 to 481
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell (PubMed:11923287, PubMed:12324459). This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments (PubMed:11923287, PubMed:12324459). The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3) (PubMed:11923287, PubMed:12324459). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.
- Sequence similarities
Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PI4K2B also known as Phosphatidylinositol 4-kinase type 2 beta is an enzyme with a molecular mass of approximately 55 kDa. This protein plays a role in the phosphorylation of phosphatidylinositol converting it to phosphatidylinositol 4-phosphate (PI4P) an important step in the phosphatidylinositol signaling pathway. PI4K2B shows expression in a variety of human tissues with particularly high levels noted in the placenta and fibroblasts. Its activity influences the formation of different cellular membranes impacting various cellular processes.
- Biological function summary
PI4K2B influences multiple cellular functions particularly those related to membrane trafficking and cell signaling. Although not part of a larger known complex PI4K2B's enzymatic production of PI4P has many downstream effects impacting endosomal dynamics and vesicular trafficking. The protein's involvement in membrane dynamics suggests important roles in regulating cell movement and recycling of membrane components affecting how cells respond to their surroundings.
- Pathways
PI4K2B integrates into the broader phosphoinositide pathway that regulates cellular communication and processes. It closely interacts with proteins like AKT and RAB proteins which are essential in the regulation of vesicle transport and signal transduction. The production of PI4P by PI4K2B contributes to the modulation of the PI3K/AKT signaling pathway impacting cellular growth survival and metabolism. This pathway's complexity demonstrates PI4K2B's role in coordinating multiple biological reactions.
- Associated diseases and disorders
Studies link altered PI4K2B function to neurodegenerative diseases and cancer. Its aberrant activity influences the signaling pathways that contribute to the development and progression of these conditions. In the context of neurodegeneration PI4K2B interacts with proteins such as tau influencing the balance of endosomal transport in neurons. In cancer changes in its activity affect cellular proliferation and survival showing connections to other oncogenic proteins within signaling cascades. These implications highlight the importance of understanding PI4K2B's roles in health and disease.
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2 product images
Functional Studies - Recombinant human PI4K2B protein (Active) (ab268861)
The specific activity of ab268861 was 1,573 nmol/min/mg in a kinase assay using PI:PS as substrate.
SDS-PAGE - Recombinant human PI4K2B protein (Active) (ab268861)
SDS-PAGE analysis of ab268861.
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