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AB162850

Recombinant Human PICH protein (GST tag N-Terminus)

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Recombinant Human PICH protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 419 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

PICH, ERCC6L, DNA excision repair protein ERCC-6-like, ATP-dependent helicase ERCC6-like, PLK1-interacting checkpoint helicase, Tumor antigen BJ-HCC-15

1 Images
SDS-PAGE - Recombinant Human PICH protein (GST tag N-Terminus) (AB162850)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PICH protein (GST tag N-Terminus) (AB162850)

ab162850 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q2NKX8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB88560

Anti-PICH antibody

Western blot - Anti-PICH antibody (AB88560)

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Proteins & Peptides

AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

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Proteins & Peptides

AB162402

Recombinant Human Zinc finger protein 691 (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Zinc finger protein 691 (GST tag N-Terminus) (AB162402)

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Proteins & Peptides

AB161146

Recombinant Human BS69 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human BS69 protein (GST tag N-Terminus) (AB161146)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as ERCC6L.
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Sequence info

[{"sequence":"MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":419,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q2NKX8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PICH also known as Plk1-interacting checkpoint helicase is an important factor in maintaining chromosome integrity during cell division. PICH is a helicase with a molecular mass of approximately 135 kDa. This protein is mainly expressed in tissues with a high rate of cell division. It possesses ATPase activity which allows it to unwind DNA structures playing a mechanical role in chromosome segregation. The helicase activity of PICH is necessary in mitosis where it interacts with centromeres during spindle assembly checkpoint activation.
Biological function summary

PICH contributes to the resolution of ultra-fine DNA bridges which occur during the late stages of mitosis. These DNA bridges can compromise genome stability leading to genetic disorders if not properly resolved. PICH works with other proteins like topoisomerase II and Bloom syndrome protein (BLM) to manage these structures. This activity suggests PICH is part of a complex necessary for protecting chromosomal stability during cell division ensuring accurate segregation of sister chromatids.

Pathways

PICH plays a role in the spindle assembly checkpoint (SAC) and mitotic exit pathway. Within these pathways PICH interacts with proteins such as Polo-like kinase 1 (Plk1) and Aurora B both important for the regulation of mitosis. PICH's interaction with these proteins highlights its significance in controlling the proper timing and execution of chromosome separation which is essential for correct mitotic progression and prevention of aneuploidy.

PICH is associated with conditions that involve genomic instability like cancer. Aberrations in PICH function can result in incorrect chromosome segregation contributing to tumorigenesis. Additionally PICH is linked with disorders involving defective DNA repair mechanisms such as those involving BRCA1 a protein important for repairing DNA double-strand breaks. Dysfunctional PICH leads to enhanced chromosomal aberrations highlighting its relevance in maintaining genomic integrity and linking it to significant pathological states.
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Specifications

Form

Liquid

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General info

Function

DNA helicase that acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase (PubMed : 17218258, PubMed : 23973328). Functions as ATP-dependent DNA translocase (PubMed : 23973328, PubMed : 28977671). Can promote Holliday junction branch migration (in vitro) (PubMed : 23973328).

Sequence similarities

Belongs to the SNF2/RAD54 helicase family.

Post-translational modifications

Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.

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Product protocols

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Target data

DNA helicase that acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase (PubMed : 17218258, PubMed : 23973328). Functions as ATP-dependent DNA translocase (PubMed : 23973328, PubMed : 28977671). Can promote Holliday junction branch migration (in vitro) (PubMed : 23973328).
See full target information ERCC6L
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