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AB95263

Recombinant Human PICOT protein

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Recombinant Human PICOT protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

PICOT, TXNL2, HUSSY-22, GLRX3, Glutaredoxin-3, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, Thioredoxin-like protein 2, PKCq-interacting protein

1 Images
SDS-PAGE - Recombinant Human PICOT protein (AB95263)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human PICOT protein (AB95263)

15% SDS-PAGE analysis of 3μg ab95263.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O76003

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as TXNL2.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAAGAAEAAVAAVEEVGSAGQFEELLRLKAKSLLVVHFWAPWAPQCAQMNEVMAELAKELPQVSFVKLEAEGVPEVSEKYEISSVPTFLFFKNSQKIDRLDGAHAPELTKKVQRHASSGSFLPSANEHLKEDLNLRLKKLTHAAPCMLFMKGTPQEPRCGFSKQMVEILHKHNIQFSSFDIFSDEEVRQGLKAYSSWPTYPQLYVSGELIGGLDIIKELEASEELDTICPKAPKLEERLKVLTNKASVMLFMKGNKQEAKCGFSKQILEILNSTGVEYETFDILEDEEVRQGLKAYSNWPTYPQLYVKGELVGGLDIVKELKENGELLPILRGEN","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O76003","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PICOT also known as thioredoxin-interacting protein (TXNIP) or Vitamin D3 up-regulated protein 1 (VDUP1) is a protein with a mass of approximately 39 kDa. It interacts with several cellular components including thioredoxin acting as a regulatory inhibitor. PICOT is expressed in a variety of tissues with notable presence in heart and muscle tissues. It influences the redox state of the cell playing a significant role in oxidative stress response.
Biological function summary

PICOT acts as a regulator of the thiol redox system in cells. It forms a complex with thioredoxin thereby inhibiting its activity which leads to an accumulation of oxidized proteins. This activity makes PICOT an important player in redox signaling affecting cellular responses to oxidative stress. Through its interactions PICOT contributes to cellular processes such as apoptosis differentiation and proliferation mediated by redox changes.

Pathways

PICOT is integral to the cellular oxidative stress response and redox regulation pathways. It interacts with proteins like thioredoxin and possibly influences NADPH oxidase activity. By modulating thioredoxin PICOT impacts the NF-kB signaling pathway which plays a role in inflammation and immune responses. These pathways highlight PICOT's involvement in maintaining cellular homeostasis especially in stress conditions.

PICOT has links to diabetes and cardiovascular disorders. It affects glucose metabolism and insulin signaling connecting to proteins such as insulin receptor substrates. PICOT also involves in heart disorders given its role in cardiac muscle stress responses and connection to proteins like troponin. Understanding these relationships can aid in developing therapeutic approaches for managing oxidative stress-related diseases.
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Specifications

Form

Liquid

Additional notes

ab95263 is purified using conventional chromatography techniques.

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General info

Function

Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (PubMed : 26613676, PubMed : 27519415). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). Required for hemoglobin maturation (PubMed : 23615448). Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.

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Product protocols

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Target data

Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (PubMed : 26613676, PubMed : 27519415). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). Required for hemoglobin maturation (PubMed : 23615448). Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.
See full target information GLRX3
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Complementary Products

Primary Antibodies

AB226396

Anti-PICOT antibody

Immunoprecipitation - Anti-PICOT antibody (AB226396)

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