Recombinant Human PIG3 protein is a Human Full Length protein, in the 1 to 332 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M L A V H F D K P G G P E N L Y V K E V A K P S P G E G E V L L K V A A S A L N R A D L M Q R Q G Q Y D P P P G A S N I L G L E A S G H V A E L G P G C Q G H W K I G D T A M A L L P G G G Q A Q Y V T V P E G L L M P I P E G L T L T Q A A A I P E A W L T A F Q L L H L V G N V Q A G D Y V L I H A G L S G V G T A A I Q L T R M A G A I P L V T A G S Q K K L Q M A E K L G A A A G F N Y K K E D F S E A T L K F T K G A G V N L I L D C I G G S Y W E K N V N C L A L D G R W V L Y G L M G G G D I N G P L F S K L L F K R G S L I T S L L R S R D N K Y K Q M L V N A F T E Q I L P H F S T E G P Q R L L P V L D R I Y P V T E I Q E A H K Y M E A N K N I G K I V L E L P Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the NADPH-dependent reduction of quinones (PubMed:19349281). Exhibits a low enzymatic activity with beta-naphthoquinones, with a strong preference for the ortho-quinone isomer (1,2-beta-naphthoquinone) over the para isomer (1,4-beta-naphthoquinone). Also displays a low reductase activity for non-quinone compounds such as diamine and 2,6-dichloroindophenol (in vitro) (PubMed:19349281). Involved in the generation of reactive oxygen species (ROS) (PubMed:19349281).
Alternative names
PIG3, TP53I3, Quinone oxidoreductase PIG3, NADPH:quinone reductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein, Protein PIG3
Recommended products
Recombinant Human PIG3 protein is a Human Full Length protein, in the 1 to 332 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q53FA7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M L A V H F D K P G G P E N L Y V K E V A K P S P G E G E V L L K V A A S A L N R A D L M Q R Q G Q Y D P P P G A S N I L G L E A S G H V A E L G P G C Q G H W K I G D T A M A L L P G G G Q A Q Y V T V P E G L L M P I P E G L T L T Q A A A I P E A W L T A F Q L L H L V G N V Q A G D Y V L I H A G L S G V G T A A I Q L T R M A G A I P L V T A G S Q K K L Q M A E K L G A A A G F N Y K K E D F S E A T L K F T K G A G V N L I L D C I G G S Y W E K N V N C L A L D G R W V L Y G L M G G G D I N G P L F S K L L F K R G S L I T S L L R S R D N K Y K Q M L V N A F T E Q I L P H F S T E G P Q R L L P V L D R I Y P V T E I Q E A H K Y M E A N K N I G K I V L E L P Q
- Accession
- Q53FA7
- Protein length
- Full Length
- Predicted molecular weight
- 37.6 kDa
- Amino acids
- 1 to 332
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab95857 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the NADPH-dependent reduction of quinones (PubMed:19349281). Exhibits a low enzymatic activity with beta-naphthoquinones, with a strong preference for the ortho-quinone isomer (1,2-beta-naphthoquinone) over the para isomer (1,4-beta-naphthoquinone). Also displays a low reductase activity for non-quinone compounds such as diamine and 2,6-dichloroindophenol (in vitro) (PubMed:19349281). Involved in the generation of reactive oxygen species (ROS) (PubMed:19349281).
- Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PIG3 also known as PIG00 or P@IG3 str0h is a protein linked to cellular stress response with a molecular mass of approximately 55 kDa. It shows expression mainly in tissues that undergo oxidative stress or DNA damage acting as an important player in apoptosis. PIG3 is mainly known as p53-induced gene 3 aligning with its dependency on p53 for activation. High expression levels become evident in tissues encountering high metabolic or environmental stress.
- Biological function summary
PIG3 mediates cell apoptosis in response to DNA damage and oxidative stress. It forms part of a complex with p53 contributing to the regulation of apoptotic pathways. As an oxidoreductase PIG3 initiates pro-apoptotic changes within the cell. Its enzymatic activity involves producing reactive oxygen species (ROS) further amplifying the cell’s apoptotic initiations. Its role connects to cellular response mechanisms in stress conditions dealing with damaged or unhealthy cells.
- Pathways
PIG3 operates within the important p53 apoptotic pathway and the DNA damage signaling pathway. It significantly interacts with p53 as PIG3 expression depends on p53 transcription factor activation. Also PIG3 connects with caspase pathways by influencing the downstream apoptosis cascade required for cell death. It acts as a link bridging initial DNA damage response and the execution of apoptosis providing a control node within these pathways.
- Associated diseases and disorders
Research links PIG3 to cancer and neurodegenerative diseases. In cancer its expression may alter affecting cell apoptosis and proliferation balance and interacting closely with proteins like p53 and BAX. In neurodegenerative disorders abnormal PIG3 activity couples with oxidative stress that leads to neuronal degeneration. Its interplay with p53 remains significant as p53's regulation of apoptotic pathways impacts numerous conditions where PIG3's role becomes equally affected.
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1 product image
SDS-PAGE - Recombinant Human PIG3 protein (ab95857)
15% SDS-PAGE analysis of 3μg ab95857.
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