JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB176047

Recombinant Human PIH1D1 protein (denatured)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human PIH1D1 protein (denatured) is a Human Full Length protein, in the 1 to 290 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

NOP17, PIH1D1, PIH1 domain-containing protein 1, Nucleolar protein 17 homolog

1 Images
SDS-PAGE - Recombinant Human PIH1D1 protein (denatured) (AB176047)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PIH1D1 protein (denatured) (AB176047)

15% SDS-PAGE analysis of ab176047 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NWS0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMANPKLLGMGLSEAEAIGADSARFEELLLQASKELQQAQTTRPESTQIQPQPGFCIKTNSSEGKVFINICHSPSIPPPADVTEEELLQMLEEDQAGFRIPMSLGEPHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRELVITIAREGLEDKYNLQLNPEWRMMKNRPFMGSISQQNIRSEQRPRIQELGDLYTPAPGRAESGPEKPHLNLWLEAPDLLLAEVDLPKLDGALGLSLEIGENRLVMGGPQQLYHLDAYIPLQINSHESKAAFHRKRKQLMVAMPLLPVPS","proteinLength":"Full Length","predictedMolecularWeight":"34.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":290,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NWS0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIH1D1 also known as PIH1 domain containing protein 1 plays a mechanical role in stabilizing multi-protein complexes. It weighs approximately 37 kDa. The protein exists mainly within the cytoplasm and nucleus of cells. PIH1D1 is a critical component of the R2TP complex contributing to its structural integrity and function. The R2TP complex plays a significant role in various cellular processes including transcription and the assembly of complexes like snoRNPs.
Biological function summary

PIH1D1 interacts with other proteins to mediate molecular chaperone activities. It forms part of the R2TP complex which stands for RNA polymerase II-associated protein 2; TP is related to tuberous sclerosis complexes. Together with other proteins such as RPAP3 it assists in assembling large multi-subunit complexes. This function is essential in maintaining normal cellular operations and stress responses facilitating the maturation and stabilization of many other proteins.

Pathways

PIH1D1 involves itself in the molecular chaperone network and mTOR signaling pathway. It interacts primarily with proteins related to the chaperone HSP90 where it helps to fold and stabilize newly synthesized proteins. The contact with mTORC1 complex which connects to growth and metabolic pathways illustrates its significant contributions to cell growth regulation and energy status sensing. PIH1D1’s role ensures that the pathways remain functional and adaptable.

Researchers have linked PIH1D1 to certain types of cancer and neurodevelopmental disorders. Its interaction with the tumor suppressor protein TSC1 suggests relevance in cancer pathogenesis where different mutations can disrupt normal cell growth control. Moreover piH1D1's involvement in the assembly of snoRNPs connects it to neurodevelopmental disorders where correct RNA processing is essential. Changes in its function can lead to significant cellular abnormalities contributing to disease progression.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles (PubMed : 17636026). Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription (PubMed : 22368283, PubMed : 24036451). Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1 (PubMed : 20864032). Positively regulates the assembly and activity of the mTORC1 complex (PubMed : 24036451).

Sequence similarities

Belongs to the PIH1 family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles (PubMed : 17636026). Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription (PubMed : 22368283, PubMed : 24036451). Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1 (PubMed : 20864032). Positively regulates the assembly and activity of the mTORC1 complex (PubMed : 24036451).
See full target information PIH1D1
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB177641

Recombinant Human XRCC2 protein (denatured)

SDS-PAGE - Recombinant Human XRCC2 protein (denatured) (AB177641)

  • 0
  • 0
Proteins & Peptides

AB174559

Recombinant Human SSSCA1 protein (denatured)

SDS-PAGE - Recombinant Human SSSCA1 protein (denatured) (AB174559)

  • 0
  • 0
Proteins & Peptides

AB177632

Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured)

SDS-PAGE - Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured) (AB177632)

  • 0
  • 0
Proteins & Peptides

AB176082

Recombinant Human CGT protein (denatured)

SDS-PAGE - Recombinant Human CGT protein (denatured) (AB176082)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com