JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181913

Recombinant Human PIM2 protein

1

(1 Review)

|

(0 Publication)

Recombinant Human PIM2 protein is a Human Full Length protein, in the 1 to 311 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Serine/threonine-protein kinase pim-2, Pim-2h, PIM2

1 Images
SDS-PAGE - Recombinant Human PIM2 protein (AB181913)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PIM2 protein (AB181913)

15% SDS-PAGE analysis of ab181913 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9P1W9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMLTKPLQGPPAPPGTPTPPPGGKDREAFEAEYRLGPLLGKGGFGTVFAGHRLTDRLQVAIKVIPRNRVLGWSPLSDSVTCPLEVALLWKVGAGGGHPGVIRLLDWFETQEGFMLVLERPLPAQDLFDYITEKGPLGEGPSRCFFGQVVAAIQHCHSRGVVHRDIKDENILIDLRRGCAKLIDFGSGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPATVWSLGILLYDMVCGDIPFERDQEILEAELHFPAHVSPDCCALIRRCLAPKPSSRPSLEEILLDPWMQTPAEDVPLNPSKGGPAPLAWSLLP","proteinLength":"Full Length","predictedMolecularWeight":"36.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":311,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9P1W9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIM2 also known as Pim-2 proto-oncogene encodes a serine/threonine kinase with a molecular weight of approximately 34 kDa. This protein is expressed in various tissues with higher levels in hematopoietic and lymphoid systems. PIM2 kinase activity increases under certain cellular stress conditions and it functions by phosphorylating substrates involved in cell survival proliferation and apoptosis regulation. As a member of the PIM kinase family it shares functional characteristics with its relatives PIM1 and PIM3.
Biological function summary

The actions of PIM2 influence cell cycle progression and survival. It does not form a complex but interacts with several other proteins. PIM2 phosphorylates important substrates such as BAD and 4E-BP1 which impacts the cellular translation machinery and apoptotic pathways. Through these interactions PIM2 contributes to cellular responses to growth factors and various stress signals promoting cell survival even in low nutrient or hypoxic conditions.

Pathways

In the PI3K/AKT and MYC signaling pathways PIM2 has important roles. PIM2 contributes by regulating the stability and activity of MYC a transcription factor vital for cancer cell metabolism. In the PI3K/AKT pathway PIM2 activity works synergistically with AKT to enhance pro-survival and proliferative signals. These actions link PIM2 with both cellular growth and survival pathways often cooperating with proteins like mTOR and BAD to modulate downstream effects.

Aberrant PIM2 expression has been associated with several malignancies including leukemia and multiple myeloma. The elevated PIM2 levels in cancer cells often correlate with resistance to apoptosis contributing to tumor progression. In these contexts PIM2 interacts with oncogenic proteins such as MYC and mTOR establishing a network that supports the survival and growth of tumor cells. Targeting PIM2 directly or its associated pathways offers potential therapeutic strategies for these cancers.
style
left-column

Specifications

Form

Liquid

Additional notes

ab181913 was purified by using conventional chromatography techniques.

style
left-column

General info

Function

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through : the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.

Post-translational modifications

Autophosphorylated.

style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through : the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
See full target information PIM2
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Proteins & Peptides

AB60836

Recombinant human PIM2 protein

proteins-peptides

recombinant-human-pim2-protein-ab60836

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com