Recombinant Human PIM2 protein is a Human Full Length protein, in the 1 to 311 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M L T K P L Q G P P A P P G T P T P P P G G K D R E A F E A E Y R L G P L L G K G G F G T V F A G H R L T D R L Q V A I K V I P R N R V L G W S P L S D S V T C P L E V A L L W K V G A G G G H P G V I R L L D W F E T Q E G F M L V L E R P L P A Q D L F D Y I T E K G P L G E G P S R C F F G Q V V A A I Q H C H S R G V V H R D I K D E N I L I D L R R G C A K L I D F G S G A L L H D E P Y T D F D G T R V Y S P P E W I S R H Q Y H A L P A T V W S L G I L L Y D M V C G D I P F E R D Q E I L E A E L H F P A H V S P D C C A L I R R C L A P K P S S R P S L E E I L L D P W M Q T P A E D V P L N P S K G G P A P L A W S L L P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
Alternative names
Serine/threonine-protein kinase pim-2, Pim-2h, PIM2
Recommended products
Recombinant Human PIM2 protein is a Human Full Length protein, in the 1 to 311 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9P1W9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M L T K P L Q G P P A P P G T P T P P P G G K D R E A F E A E Y R L G P L L G K G G F G T V F A G H R L T D R L Q V A I K V I P R N R V L G W S P L S D S V T C P L E V A L L W K V G A G G G H P G V I R L L D W F E T Q E G F M L V L E R P L P A Q D L F D Y I T E K G P L G E G P S R C F F G Q V V A A I Q H C H S R G V V H R D I K D E N I L I D L R R G C A K L I D F G S G A L L H D E P Y T D F D G T R V Y S P P E W I S R H Q Y H A L P A T V W S L G I L L Y D M V C G D I P F E R D Q E I L E A E L H F P A H V S P D C C A L I R R C L A P K P S S R P S L E E I L L D P W M Q T P A E D V P L N P S K G G P A P L A W S L L P
- Accession
- Q9P1W9
- Protein length
- Full Length
- Predicted molecular weight
- 36.6 kDa
- Amino acids
- 1 to 311
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab181913 was purified by using conventional chromatography techniques.
General info
- Function
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
- Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
- Post-translational modifications
Autophosphorylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PIM2 also known as Pim-2 proto-oncogene encodes a serine/threonine kinase with a molecular weight of approximately 34 kDa. This protein is expressed in various tissues with higher levels in hematopoietic and lymphoid systems. PIM2 kinase activity increases under certain cellular stress conditions and it functions by phosphorylating substrates involved in cell survival proliferation and apoptosis regulation. As a member of the PIM kinase family it shares functional characteristics with its relatives PIM1 and PIM3.
- Biological function summary
The actions of PIM2 influence cell cycle progression and survival. It does not form a complex but interacts with several other proteins. PIM2 phosphorylates important substrates such as BAD and 4E-BP1 which impacts the cellular translation machinery and apoptotic pathways. Through these interactions PIM2 contributes to cellular responses to growth factors and various stress signals promoting cell survival even in low nutrient or hypoxic conditions.
- Pathways
In the PI3K/AKT and MYC signaling pathways PIM2 has important roles. PIM2 contributes by regulating the stability and activity of MYC a transcription factor vital for cancer cell metabolism. In the PI3K/AKT pathway PIM2 activity works synergistically with AKT to enhance pro-survival and proliferative signals. These actions link PIM2 with both cellular growth and survival pathways often cooperating with proteins like mTOR and BAD to modulate downstream effects.
- Associated diseases and disorders
Aberrant PIM2 expression has been associated with several malignancies including leukemia and multiple myeloma. The elevated PIM2 levels in cancer cells often correlate with resistance to apoptosis contributing to tumor progression. In these contexts PIM2 interacts with oncogenic proteins such as MYC and mTOR establishing a network that supports the survival and growth of tumor cells. Targeting PIM2 directly or its associated pathways offers potential therapeutic strategies for these cancers.
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1 product image
SDS-PAGE - Recombinant Human PIM2 protein (ab181913)
15% SDS-PAGE analysis of ab181913 (3μg).
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