JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB204146

Recombinant human PIM3 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human PIM3 protein is a Human Full Length protein, in the 1 to 326 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Serine/threonine-protein kinase pim-3, PIM3

2 Images
Functional Studies - Recombinant human PIM3 protein (AB204146)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PIM3 protein (AB204146)

Sample Kinase Activity Plot. ab204146 specific activity was determined to be 900 nmol/min/mg.

SDS-PAGE - Recombinant human PIM3 protein (AB204146)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PIM3 protein (AB204146)

SDS-PAGE analysis of ab204146. MW 65 KDa.

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity is 900 mol/min/mg.

Accession

Q86V86

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB198842

Anti-PIM3 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM3 antibody - C-terminal (AB198842)

  • 0
  • 0
Biochemicals

AB141591

STO-609 acetate, CaM-KK inhibitor

Chemical Structure - STO-609 acetate, CaM-KK inhibitor (AB141591)

  • 0
  • 0
Proteins & Peptides

AB235719

Recombinant Human GLP-1 protein (Tagged)

SDS-PAGE - Recombinant Human GLP-1 protein (Tagged) (AB235719)

  • 0
  • 0
Proteins & Peptides

AB105593

Recombinant Human Iba1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Iba1 protein (His tag N-Terminus) (AB105593)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
style
left-column

Sequence info

[{"sequence":"MLLSKFGSLAHLCGPGGVDHLPVKILQPAKADKESFEKAYQVGAVLGSGGFGTVYAGSRIADGLPVAVKHVVKERVTEWGSLGGATVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFDFITERGALDEPLARRFFAQVLAAVRHCHSCGVVHRDIKDENLLVDLRSGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLLFRRRVSPECQQLIRWCLSLRPSERPSLDQIAAHPWMLGADGGVPESCDLRLCTLDPDDVASTTSSSESL","proteinLength":"Full Length","predictedMolecularWeight":"65 kDa","actualMolecularWeight":null,"aminoAcidEnd":326,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q86V86","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PIM3 protein is a member of the proviral integration site for moloney murine leukemia virus (PIM) serine/threonine kinase family. It is also known as PIM-3 kinase. This protein has a mass of approximately 37 kDa. Expression of PIM3 is found in various tissues including liver pancreas kidney and some cancer cell lines. Scientists recognize its role in phosphorylating target proteins which affects various cellular processes like cell cycle and apoptosis.
Biological function summary

PIM3 functions by regulating cell survival and proliferation. It does not form part of any large protein complex but operates through phosphorylation of substrates. This kinase modulates signaling pathways important for mitogenic stimuli and cellular responses to various stress factors. Tissues with abnormal PIM3 levels may experience altered cell growth and survival which can affect normal physiological processes.

Pathways

PIM3 interacts with cellular signaling networks. It is notably involved in the PI3K/AKT signaling pathway affecting cell growth and metabolism. Additionally PIM3 participates in mTOR signaling linking it to protein synthesis and cellular energy balance. It shows functional relation with proteins such as AKT and mTOR suggesting its integration in these pivotal regulatory circuits.

Excessive PIM3 expression associates with certain types of cancer such as pancreatic cancer and hepatocellular carcinoma. It contributes to oncogenic processes by promoting uncontrolled cell growth and survival. In cancer contexts PIM3 interacts with oncogenes like c-MYC which may exacerbate tumorous characteristics. Targeting PIM3 pathways offers potential therapeutic strategies for related malignancies.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through : the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.

Post-translational modifications

Ubiquitinated, leading to proteasomal degradation.. Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.

style
left-column

Product protocols

style
left-column

Target data

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through : the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.
See full target information PIM3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com