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AB116807

Recombinant Human PINCH 1 protein

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Recombinant Human PINCH 1 protein is a Human protein, in the 1 to 325 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

PINCH, PINCH1, LIMS1, LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, Renal carcinoma antigen NY-REN-48, PINCH-1

1 Images
SDS-PAGE - Recombinant Human PINCH 1 protein (AB116807)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PINCH 1 protein (AB116807)

12.5% SDS-PAGE showing ab116807 at approximately 61.82kDa.
Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P48059

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein.</p>" } } }
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Product details

This product was previously labelled as LIMS1.
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Sequence info

[{"sequence":"MANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGDVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLAETLGRK","proteinLength":null,"predictedMolecularWeight":"61.82 kDa","actualMolecularWeight":null,"aminoAcidEnd":325,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P48059","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PINCH 1 also known as LIMS1 is a protein with a molecular mass of approximately 35 kDa. It serves an important role in cellular adhesion and signaling as part of the focal adhesion complex. Expressed widely in many tissues PINCH 1 interacts with integrin-linked kinase (ILK) and parvin proteins to mediate interactions between the extracellular matrix and the actin cytoskeleton. This interaction supports cell adhesion migration and mechanical stability critical for many cellular processes.
Biological function summary

PINCH 1 facilitates communication within cells by anchoring the cell's structural components to its signaling pathways. It forms the ternary PINCH-ILK-parvin (PIP) complex essential for transmitting mechanical and chemical signals within the cell. This complex enables cells to respond to their environments adjusting their activities accordingly. PINCH 1 also participates in the regulation of cell survival and proliferation suggesting its importance in maintaining cellular functions.

Pathways

PINCH 1 acts as a participant in the integrin signaling and focal adhesion pathways. In these pathways PINCH 1 works alongside proteins like ILK and alpha-parvin to translate extracellular matrix signals into intracellular responses. These pathways regulate cell movement shape changes and survival by coordinating actin cytoskeleton remodeling and integrin-mediated adhesion. This coordination allows cells to adjust their behavior based on external cues.

PINCH 1 has associations with cancer and cardiovascular diseases where its regulatory role in cell adhesion and signaling becomes evident. Abnormal PINCH 1 expression or function may lead to impaired cell adhesion and increased cell motility contributing to cancer metastasis. In cardiovascular diseases disruptions in PINCH 1 interactions might affect heart tissues' biomechanical integrity through pathways involving ILK. Proper PINCH 1 activity is critical for maintaining the stability and function of heart and tissue cells.
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Specifications

Form

Liquid

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General info

Function

Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.

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Product protocols

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Target data

Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.
See full target information LIMS1
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Complementary Products

Primary Antibodies

AB76112

Anti-PINCH 1 + PINCH 2 antibody [EP1943Y]

RabMAb

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (AB76112)

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