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AB82088

Recombinant Human PIP4K2 beta protein

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Recombinant Human PIP4K2 beta protein is a Human Full Length protein, expressed in Baculovirus, with >95%.

View Alternative Names

PIP5K2B, PIP4K2B, Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PtdIns(5)P-4-kinase isoform 2-beta, PI(5)P 4-kinase type II beta, PIP4KII-beta

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus

Tags

Tag free

Biologically active

No

Accession

P78356

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.9 Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA

storage-buffer

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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P78356","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIP4K2 beta also referred to as phosphatidylinositol-5-phosphate 4-kinase type-2 beta is an enzyme that functions in the phosphorylation of phosphatidylinositol-5-phosphate (PI5P) to produce phosphatidylinositol-45-bisphosphate (PI(45)P2). The molecular mass of PIP4K2 beta is approximately 47 kDa. This kinase has important roles in cell signaling processes and maintains its activity across various tissues with expression noticeable in the brain liver and immune system cells.
Biological function summary

The generation of PI(45)P2 by PIP4K2 beta plays a critical role in membrane dynamics and actin cytoskeleton regulation. This enzyme contributes to the control of intracellular signaling by affecting pathways involved in cell growth survival and migration. PIP4K2 beta functions as part of larger protein complexes which can affect its activity and interactions coordinating cellular responses under different physiological conditions.

Pathways

PIP4K2 beta integrates into the phosphoinositide signaling pathway and influences the PI3K/AKT/mTOR signaling cascade imperative for cell survival and growth. It interacts with other kinases such as PI3K to regulate the availability of lipid signaling molecules. The activity of PIP4K2 beta is also linked to the regulation of insulin signaling working alongside phosphatases that modulate PI(45)P2 and its derivatives.

Dysregulation of PIP4K2 beta activity can associate with metabolic disorders like type 2 diabetes and is observed in certain cancer types. For instance abnormal expression levels can affect cellular proliferation and glucose metabolism highlighting its role in insulin signaling pathways. Additionally the interaction of PIP4K2 beta with proteins such as AKT an important player in cancer pathways highlights its potential impact on tumorigenesis and the development of insulin resistance.
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Specifications

Form

Liquid

Additional notes

The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.

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General info

Function

Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate (PubMed : 26774281, PubMed : 9038203). Preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation and its activity reflects changes in direct proportion to the physiological GTP concentration (PubMed : 26774281). Its GTP-sensing activity is critical for metabolic adaptation (PubMed : 26774281). PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism. They interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3 (PubMed : 31091439).

Post-translational modifications

Ubiquitinated by the SPOP/CUL3 complex. Ubiquitination is stimulated by PtdIns5P levels.. Phosphorylated on serine residues.

Subcellular localisation

Nucleus

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate (PubMed : 26774281, PubMed : 9038203). Preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation and its activity reflects changes in direct proportion to the physiological GTP concentration (PubMed : 26774281). Its GTP-sensing activity is critical for metabolic adaptation (PubMed : 26774281). PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism. They interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3 (PubMed : 31091439).
See full target information PIP4K2B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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