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AB268871

Recombinant human PIP5K1 beta protein (Active)

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Recombinant human PIP5K1 beta protein (Active) is a Human Full Length protein, in the 1 to 540 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

View Alternative Names

STM7, PIP5K1B, Phosphatidylinositol 4-phosphate 5-kinase type-1 beta, PIP5K1-beta, PtdIns(4)P-5-kinase 1 beta, Phosphatidylinositol 4-phosphate 5-kinase type I beta, Protein STM-7, Type I phosphatidylinositol 4-phosphate 5-kinase beta, PIP5KIbeta

2 Images
Functional Studies - Recombinant human PIP5K1 beta protein (Active) (AB268871)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PIP5K1 beta protein (Active) (AB268871)

The specific activity of ab268871 was 3,400 nmol/min/mg in a kinase assay using PI(4)P : PS as substrate.

SDS-PAGE - Recombinant human PIP5K1 beta protein (Active) (AB268871)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PIP5K1 beta protein (Active) (AB268871)

SDS-PAGE analysis of ab268871.

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab268871 was 3,400 nmol/min/mg in a kinase assay using PI(4)P:PS as substrate.

Accession

O14986

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSSAAENGEAAPGKQNEEKTYKKTASSAIKGAIQLGIGYTVGNLTSKPERDVLMQDFYVVESVFLPSEGSNLTPAHHYPDFRFKTYAPLAFRYFRELFGIKPDDYLYSICSEPLIELSNPGASGSLFFVTSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCMQSGGINIRIVVMNNVLPRSMRMHFTYDLKGSTYKRRASRKEREKSNPTFKDLDFLQDMHEGLYFDTETYNALMKTLQRDCRVLESFKIMDYSLLLGIHFLDHSLKEKEEETPQNVPDAKRTGMQKVLYSTAMESIQGPGKSGDGIITENPDTMGGIPAKSHRGEKLLLFMGIIDILQSYRLMKKLEHSWKALVYDGDTVSVHRPSFYADRFLKFMNSRVFKKIQALKASPSKKRCNSIAALKATSQEIVSSISQEWKDEKRDLLTEGQSFSSLDEEALGSRHRPDLVPSTPSLFEAASLATTISSSSLYVNEHYPHDRPTLYSNSKGLPSSSTFTLEEGTIYLTAEPNTLEVQDDNASVLDVYL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":540,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O14986","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The phosphatidylinositol-4-phosphate 5-kinase type 1 beta (PIP5K1β) also known as PIPK type I beta is an enzyme that plays an important role in the synthesis of phosphatidylinositol 45-bisphosphate (PIP2) from phosphatidylinositol 4-phosphate (PI4P). This enzyme has a molecular weight of approximately 68 kDa. It expresses across various tissues with significant presence in the brain and in hematopoietic cells. The PIP5K1β enzyme is important in regulating the levels of PIP2 a critical lipid in membrane dynamics and signaling.
Biological function summary

PIP5K1β acts within the phosphoinositide signaling pathway which is essential for a range of cellular processes including actin cytoskeleton organization vesicle trafficking and cell survival. It forms part of a complex with other kinases and phosphatases that modulate cellular lipid concentrations. PIP5K1β also contributes to signal transduction processes by participating in the recruitment and activation of proteins that respond to membrane-associated signals.

Pathways

PIP5K1β is heavily involved in the regulation of the phosphoinositide 3-kinase (PI3K) pathway and the actin cytoskeleton pathway. Its product PIP2 serves as a substrate for PI3K which generates phosphatidylinositol-345-trisphosphate (PIP3) an important signaling molecule. PIP5K1β therefore indirectly impacts pathways that involve proteins like AKT and Rac1 which are important to cellular growth and motility.

PIP5K1β connects to cancer and immune system-related disorders. Dysregulation of PIP5K1β activity can lead to aberrant PI3K signaling contributing to oncogenic processes. Additionally it affects proteins such as AKT which plays a role in tumor progression and survival. In immune disorders altered PIP5K1β levels can impact the actin cytoskeleton affecting immune cell dynamics and function.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (By similarity). PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers : inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (By similarity). Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of phospholipase PLD2. Together with PIP5K1A, is required, after stimulation by G-protein coupled receptors, for the synthesis of IP3 that will induce stable platelet adhesion (By similarity).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (By similarity). PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers : inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (By similarity). Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of phospholipase PLD2. Together with PIP5K1A, is required, after stimulation by G-protein coupled receptors, for the synthesis of IP3 that will induce stable platelet adhesion (By similarity).
See full target information PIP5K1B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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