Recombinant human PIP5K1C protein (Active) is a Human Full Length protein, in the 1 to 668 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M E L E V P D E A E S A E A G A V P S E A A W A A E S G A A A G L A Q K K A A P T E V L S M T A Q P G P G H G K K L G H R G V D A S G E T T Y K K T T S S T L K G A I Q L G I G Y T V G H L S S K P E R D V L M Q D F Y V V E S I F F P S E G S N L T P A H H F Q D F R F K T Y A P V A F R Y F R E L F G I R P D D Y L Y S L C N E P L I E L S N P G A S G S L F Y V T S D D E F I I K T V M H K E A E F L Q K L L P G Y Y M N L N Q N P R T L L P K F Y G L Y C V Q S G G K N I R V V V M N N I L P R V V K M H L K F D L K G S T Y K R R A S K K E K E K S F P T Y K D L D F M Q D M P E G L L L D A D T F S A L V K T L Q R D C L V L E S F K I M D Y S L L L G V H N I D Q H E R E R Q A Q G A Q S T S D E K R P V G Q K A L Y S T A M E S I Q G G A A R G E A I E S D D T M G G I P A V N G R G E R L L L H I G I I D I L Q S Y R F I K K L E H T W K A L V H D G D T V S V H R P S F Y A E R F F K F M S N T V F R K N S S L K S S P S K K G R G G A L L A V K P L G P T A A F S A S Q I P S E R E E A Q Y D L R G A R S Y P T L E D E G R P D L L P C T P P S F E E A T T A S I A T T L S S T S L S I P E R S P S E T S E Q P R Y R R R T Q S S G Q D G R P Q E E P P A E E D L Q Q I T V Q V E P A C S V E I V V P K E E D A G V E A S P A G A S A A V E V E T A S Q A S D E E G A P A S Q A S D E E D A P A T D I Y F P T D E R S W V Y S P L H Y S A Q A P P A S D G E S D T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (PubMed:12422219, PubMed:22942276). PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (Probable). PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis (By similarity). Together with PIP5K1A, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps (By similarity). Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport (By similarity). Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis (PubMed:12847086). Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2) (PubMed:12847086). Required for clathrin-coated pits assembly at the synapse (PubMed:17261850). Participates in cell junction assembly (PubMed:17261850). Modulates adherens junctions formation by facilitating CDH1/cadherin trafficking (PubMed:17261850). Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions (PubMed:12422219). Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins (PubMed:12422219). Required for uropodium formation and retraction of the cell rear during directed migration (By similarity). Has a role in growth factor-stimulated directional cell migration and adhesion (By similarity). Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor (PubMed:17635937). Negative regulator of T-cell activation and adhesion (By similarity). Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor (By similarity). Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth (By similarity).
Alternative names
KIAA0589, PIP5K1C, Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma, PIP5K1gamma, PtdIns(4)P-5-kinase 1 gamma, Type I phosphatidylinositol 4-phosphate 5-kinase gamma
Recommended products
Recombinant human PIP5K1C protein (Active) is a Human Full Length protein, in the 1 to 668 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268872 was 6,927 nmol/min/mg in a kinase assay using PI(4)P:PS as substrate.
- Accession
- O60331-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M E L E V P D E A E S A E A G A V P S E A A W A A E S G A A A G L A Q K K A A P T E V L S M T A Q P G P G H G K K L G H R G V D A S G E T T Y K K T T S S T L K G A I Q L G I G Y T V G H L S S K P E R D V L M Q D F Y V V E S I F F P S E G S N L T P A H H F Q D F R F K T Y A P V A F R Y F R E L F G I R P D D Y L Y S L C N E P L I E L S N P G A S G S L F Y V T S D D E F I I K T V M H K E A E F L Q K L L P G Y Y M N L N Q N P R T L L P K F Y G L Y C V Q S G G K N I R V V V M N N I L P R V V K M H L K F D L K G S T Y K R R A S K K E K E K S F P T Y K D L D F M Q D M P E G L L L D A D T F S A L V K T L Q R D C L V L E S F K I M D Y S L L L G V H N I D Q H E R E R Q A Q G A Q S T S D E K R P V G Q K A L Y S T A M E S I Q G G A A R G E A I E S D D T M G G I P A V N G R G E R L L L H I G I I D I L Q S Y R F I K K L E H T W K A L V H D G D T V S V H R P S F Y A E R F F K F M S N T V F R K N S S L K S S P S K K G R G G A L L A V K P L G P T A A F S A S Q I P S E R E E A Q Y D L R G A R S Y P T L E D E G R P D L L P C T P P S F E E A T T A S I A T T L S S T S L S I P E R S P S E T S E Q P R Y R R R T Q S S G Q D G R P Q E E P P A E E D L Q Q I T V Q V E P A C S V E I V V P K E E D A G V E A S P A G A S A A V E V E T A S Q A S D E E G A P A S Q A S D E E D A P A T D I Y F P T D E R S W V Y S P L H Y S A Q A P P A S D G E S D T
- Accession
- O60331
- Protein length
- Full Length
- Amino acids
- 1 to 668
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (PubMed:12422219, PubMed:22942276). PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (Probable). PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis (By similarity). Together with PIP5K1A, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps (By similarity). Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport (By similarity). Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis (PubMed:12847086). Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2) (PubMed:12847086). Required for clathrin-coated pits assembly at the synapse (PubMed:17261850). Participates in cell junction assembly (PubMed:17261850). Modulates adherens junctions formation by facilitating CDH1/cadherin trafficking (PubMed:17261850). Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions (PubMed:12422219). Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins (PubMed:12422219). Required for uropodium formation and retraction of the cell rear during directed migration (By similarity). Has a role in growth factor-stimulated directional cell migration and adhesion (By similarity). Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor (PubMed:17635937). Negative regulator of T-cell activation and adhesion (By similarity). Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor (By similarity). Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth (By similarity).
- Post-translational modifications
Phosphorylation on Ser-650 negatively regulates binding to TLN2 and is strongly stimulated in mitosis. Phosphorylation on Tyr-649 is necessary for targeting to focal adhesions. Phosphorylation on Ser-650 and Tyr-649 are mutually exclusive. Phosphorylated by SYK and CSK (By similarity). Tyrosine phosphorylation is enhanced by PTK2 signaling. Phosphorylated at Tyr-639 upon EGF stimulation. Some studies suggest that phosphorylation on Tyr-649 enhances binding to tailins (TLN1 and TLN2). According to PubMed:15738269 phosphorylation at Tyr-649 does not directly enhance binding to tailins (TLN1 and TLN2) but may act indirectly by inhibiting phosphorylation at Ser-650.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PIP5K1C also known as phosphatidylinositol-4-phosphate 5-kinase type-1 gamma has a mass of approximately 75 kDa. This enzyme plays a critical role in the phosphatidylinositol signaling pathway where it catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PI4P) to produce phosphatidylinositol 45-bisphosphate (PIP2). PIP5K1C's activity is pivotal for membrane trafficking and it demonstrates widespread expression in tissues including the brain and skeletal muscle.
- Biological function summary
PIP5K1C is involved in the regulation of actin cytoskeleton dynamics. It participates as an important enzyme in complexes that modulate actin polymerization and cell adhesion processes. Its function impacts cell motility and maintenance of cell shape contributing significantly to endocytosis and exocytosis. This function highlights the enzyme's role in intracellular signaling and adhesion-related activities.
- Pathways
PIP5K1C interacts intricately within the phosphoinositide signaling pathway and impacts the regulation of focal adhesion. It influences other proteins like RAC1 an important regulator of actin polymerization and talin which is involved in linking integrins to the actin cytoskeleton. These interactions place PIP5K1C as an important contributor to the molecular mechanisms that control cell movement and attachment.
- Associated diseases and disorders
Mutations or dysfunctions in PIP5K1C have been associated with neurological disorders such as Charcot-Marie-Tooth disease type 4F and L-fucosidosis. Abnormalities in PIP5K1C expression or activity can disrupt cellular functions and lead to these conditions. In the context of Charcot-Marie-Tooth disease PIP5K1C may interact with dynamin 2 a protein involved in endocytic vesicle fission which further influences the peripheral nervous system pathology.
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2 product images
Functional Studies - Recombinant human PIP5K1C protein (Active) (ab268872)
The specific activity of ab268872 was 6,927 nmol/min/mg in a kinase assay using PI(4)P:PS as substrate.
SDS-PAGE - Recombinant human PIP5K1C protein (Active) (ab268872)
SDS-PAGE analysis of ab268872.
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