Recombinant Human PIP5K3/PIKFYVE protein (Tagged) is a Human Fragment protein, in the 1493 to 2098 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
K K Q S L C E V L Q A W N N R L Q D L F Q Q E K G R K R P S V P P S P G R L R Q G E E S K I S A M D A S P R N I S P G L Q N G E K E D R F L T T L S S Q S S T S S T H L Q L P T P P E V M S E Q S V G G P P E L D T A S S S E D V F D G H L L G S T D S Q V K E K S T M K A I F A N L L P G N S Y N P I P F P F D P D K H Y L M Y E H E R V P I A V C E K E P S S I I A F A L S C K E Y R N A L E E L S K A T Q W N S A E E G L P T N S T S D S R P K S S S P I R L P E M S G G Q T N R T T E T E P Q P T K K A S G M L S F F R G T A G K S P D L S S Q K R E T L R G A D S A Y Y Q V G Q T G K E G T E N Q G V E P Q D E V D G G D T Q K K Q L I N P H V E L Q F S D A N A K F Y C R L Y Y A G E F H K M R E V I L D S S E E D F I R S L S H S S P W Q A R G G K S G A A F Y A T E D D R F I L K Q M P R L E V Q S F L D F A P H Y F N Y I T N A V Q Q K R P T A L A K I L G V Y R I G Y K N S Q N N T E K K L D L L V M E N L F Y G R K M A Q V F D L K G S L R N R N V K T D T G K E S C D V V L L D E N L L K M V R D N P L Y I R S H S K A V L R T S I H S D S H F L S S H L I I D Y S L L V G R D D T S N E L V V G I I D Y I R T F T W D K K L E M V V K S T G I L G G Q G K M P T V V S P E L Y R T R F C E A M D K Y F L M V P D H W T G L G L N C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Dual specificity kinase implicated in myriad essential cellular processes such as maintenance of endomembrane homeostasis, and endocytic-vacuolar pathway, lysosomal trafficking, nuclear transport, stress- or hormone-induced signaling and cell cycle progression (PubMed:23086417). The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Sole enzyme to catalyze the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form (PtdIns(3,5)P2) (PubMed:17556371). Also catalyzes the phosphorylation of phosphatidylinositol on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 5-phosphate (PtdIns(5)P) (PubMed:22621786). Has serine-protein kinase activity and is able to autophosphorylate and transphosphorylate. Autophosphorylation inhibits its own phosphatidylinositol 3-phosphate 5-kinase activity, stimulates FIG4 lipid phosphatase activity and down-regulates lipid product formation (PubMed:33098764). Involved in key endosome operations such as fission and fusion in the course of endosomal cargo transport (PubMed:22621786). Required for the maturation of early into late endosomes, phagosomes and lysosomes (PubMed:30612035). Regulates vacuole maturation and nutrient recovery following engulfment of macromolecules, initiates the redistribution of accumulated lysosomal contents back into the endosome network (PubMed:27623384). Critical regulator of the morphology, degradative activity, and protein turnover of the endolysosomal system in macrophages and platelets (By similarity). In neutrophils, critical to perform chemotaxis, generate ROS, and undertake phagosome fusion with lysosomes (PubMed:28779020). Plays a key role in the processing and presentation of antigens by major histocompatibility complex class II (MHC class II) mediated by CTSS (PubMed:30612035). Regulates melanosome biogenesis by controlling the delivery of proteins from the endosomal compartment to the melanosome (PubMed:29584722). Essential for systemic glucose homeostasis, mediates insulin-induced signals for endosome/actin remodeling in the course of GLUT4 translocation/glucose uptake activation (By similarity). Supports microtubule-based endosome-to-trans-Golgi network cargo transport, through association with SPAG9 and RABEPK (By similarity). Mediates EGFR trafficking to the nucleus (PubMed:17909029). (Microbial infection) Required for cell entry of coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus EMC (HCoV-EMC) by endocytosis.
Alternative names
KIAA0981, PIP5K3, PIKFYVE, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, Serine-protein kinase PIKFYVE, PIPkin-III, Type III PIP kinase
Recommended products
Recombinant Human PIP5K3/PIKFYVE protein (Tagged) is a Human Fragment protein, in the 1493 to 2098 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- Q9Y2I7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- K K Q S L C E V L Q A W N N R L Q D L F Q Q E K G R K R P S V P P S P G R L R Q G E E S K I S A M D A S P R N I S P G L Q N G E K E D R F L T T L S S Q S S T S S T H L Q L P T P P E V M S E Q S V G G P P E L D T A S S S E D V F D G H L L G S T D S Q V K E K S T M K A I F A N L L P G N S Y N P I P F P F D P D K H Y L M Y E H E R V P I A V C E K E P S S I I A F A L S C K E Y R N A L E E L S K A T Q W N S A E E G L P T N S T S D S R P K S S S P I R L P E M S G G Q T N R T T E T E P Q P T K K A S G M L S F F R G T A G K S P D L S S Q K R E T L R G A D S A Y Y Q V G Q T G K E G T E N Q G V E P Q D E V D G G D T Q K K Q L I N P H V E L Q F S D A N A K F Y C R L Y Y A G E F H K M R E V I L D S S E E D F I R S L S H S S P W Q A R G G K S G A A F Y A T E D D R F I L K Q M P R L E V Q S F L D F A P H Y F N Y I T N A V Q Q K R P T A L A K I L G V Y R I G Y K N S Q N N T E K K L D L L V M E N L F Y G R K M A Q V F D L K G S L R N R N V K T D T G K E S C D V V L L D E N L L K M V R D N P L Y I R S H S K A V L R T S I H S D S H F L S S H L I I D Y S L L V G R D D T S N E L V V G I I D Y I R T F T W D K K L E M V V K S T G I L G G Q G K M P T V V S P E L Y R T R F C E A M D K Y F L M V P D H W T G L G L N C
- Accession
- Q9Y2I7
- Protein length
- Fragment
- Amino acids
- 1493 to 2098
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Dual specificity kinase implicated in myriad essential cellular processes such as maintenance of endomembrane homeostasis, and endocytic-vacuolar pathway, lysosomal trafficking, nuclear transport, stress- or hormone-induced signaling and cell cycle progression (PubMed:23086417). The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Sole enzyme to catalyze the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form (PtdIns(3,5)P2) (PubMed:17556371). Also catalyzes the phosphorylation of phosphatidylinositol on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 5-phosphate (PtdIns(5)P) (PubMed:22621786). Has serine-protein kinase activity and is able to autophosphorylate and transphosphorylate. Autophosphorylation inhibits its own phosphatidylinositol 3-phosphate 5-kinase activity, stimulates FIG4 lipid phosphatase activity and down-regulates lipid product formation (PubMed:33098764). Involved in key endosome operations such as fission and fusion in the course of endosomal cargo transport (PubMed:22621786). Required for the maturation of early into late endosomes, phagosomes and lysosomes (PubMed:30612035). Regulates vacuole maturation and nutrient recovery following engulfment of macromolecules, initiates the redistribution of accumulated lysosomal contents back into the endosome network (PubMed:27623384). Critical regulator of the morphology, degradative activity, and protein turnover of the endolysosomal system in macrophages and platelets (By similarity). In neutrophils, critical to perform chemotaxis, generate ROS, and undertake phagosome fusion with lysosomes (PubMed:28779020). Plays a key role in the processing and presentation of antigens by major histocompatibility complex class II (MHC class II) mediated by CTSS (PubMed:30612035). Regulates melanosome biogenesis by controlling the delivery of proteins from the endosomal compartment to the melanosome (PubMed:29584722). Essential for systemic glucose homeostasis, mediates insulin-induced signals for endosome/actin remodeling in the course of GLUT4 translocation/glucose uptake activation (By similarity). Supports microtubule-based endosome-to-trans-Golgi network cargo transport, through association with SPAG9 and RABEPK (By similarity). Mediates EGFR trafficking to the nucleus (PubMed:17909029).
- Post-translational modifications
Autophosphorylates which inhibits its own phosphatidylinositol 3-phosphate 5-kinase activity, stimulates FIG4 lipid phosphatase activity and down-regulates lipid product formation (PubMed:33098764). Dephosphorylated by FIG4 in the PI(3,5)P2 regulatory complex, at Ser-48, Ser-1669 and Ser-2053 (PubMed:33098764). Phosphorylated in response to insulin at Ser-318 in a protein kinase B (PKB)-dependent manner (PubMed:20513353).
- Subcellular localisation
- Endosome membrane, Early endosome membrane, Late endosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PIP5K3 also known as PIKFYVE is a lipid kinase with a significant role in the phosphorylation of phosphatidylinositol 3-phosphate to phosphatidylinositol (35)-bisphosphate. This protein weighs approximately 237 kDa and is predominantly expressed in the cytoplasm of a variety of cell types. Its enzymatic activity is essential for the maintenance of phosphatidylinositol 35-bisphosphate levels influencing membrane trafficking and endosomal function.
- Biological function summary
PIP5K3/PIKFYVE functions as an important component in regulating endosomal dynamics. It is part of a complex with VAC14 and FIG4 which plays a significant role in endosomal membrane trafficking lysosome biogenesis and autophagy. The protein's expression aids in maintaining cellular homeostasis and modulating organelle identity within the endosomal-lysosomal system which is critical for normal cellular operations.
- Pathways
PIP5K3/PIKFYVE integrates into the phosphatidylinositol signaling pathway and is important for proper endocytic trafficking. This protein interacts with key pathway constituents like Rab5 and Rab7 facilitating the transport of endosomes and impacting the process of autophagy. These interactions highlight the protein's centrality in the coordination of intracellular trafficking processes essential for cellular health.
- Associated diseases and disorders
PIP5K3/PIKFYVE mutations and dysregulation associate with rare disorders such as Charcot-Marie-Tooth disease and fleck corneal dystrophy. Abnormal functioning of PIP5K3/PIKFYVE can affect its complex partners FIG4 and VAC14 leading to neurodegenerative symptoms and defects in cell morphology. Studies reveal vulnerabilities in cellular pathways due to aberrations in this protein offering potential targets for therapeutic intervention.
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SDS-PAGE - Recombinant Human PIP5K3/PIKFYVE protein (Tagged) (ab268873)
SDS-PAGE analysis of ab268873.
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