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AB167827

Recombinant Human Pirh2 protein (denatured)

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Recombinant Human Pirh2 protein (denatured) is a Human Full Length protein, in the 1 to 261 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

ARNIP, CHIMP, PIRH2, RNF199, ZNF363, RCHY1, RING finger and CHY zinc finger domain-containing protein 1, Androgen receptor N-terminal-interacting protein, CH-rich-interacting match with PLAG1, E3 ubiquitin-protein ligase Pirh2, RING finger protein 199, RING-type E3 ubiquitin transferase RCHY1, Zinc finger protein 363, p53-induced RING-H2 protein, hPirh2

1 Images
SDS-PAGE - Recombinant Human Pirh2 protein (denatured) (AB167827)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Pirh2 protein (denatured) (AB167827)

15% SDS-PAGE analysis of 3µg ab167827.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q96PM5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQHAQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ","proteinLength":"Full Length","predictedMolecularWeight":"32.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":261,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96PM5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pirh2 also known as RCHY1 is a protein with E3 ubiquitin ligase activity. It has a molecular mass of approximately 34 kDa. Pirh2 targets proteins for degradation by the ubiquitin-proteasome system regulating the levels of proteins like p53. Expression of Pirh2 occurs in various tissues including liver and heart. It helps maintain cellular protein homeostasis by marking specific substrates for degradation.
Biological function summary

Pirh2 influences the regulation of protein stability by tagging proteins for degradation. Besides p53 it acts on other substrates and engages in various cellular processes. Pirh2 operates independently and its presence affects cellular responses to stress and DNA damage. It modulates cell cycle progression and apoptosis impacting cell fate decisions.

Pathways

Pirh2 plays an important role in the p53 signaling pathway an essential cell regulatory network. By mediating the ubiquitination of p53 Pirh2 affects cell survival and division under stress conditions. Interaction with proteins such as Mdm2 further regulates p53 activity impacting the cell's ability to respond to DNA damage and carcinogen exposure.

Research connects Pirh2 with the development of cancers. Its interaction with the tumor suppressor protein p53 poses effects on tumorigenesis by altering p53 stability and function. Overexpression of Pirh2 has connections to several cancer types including breast and liver cancers. These associations suggest Pirh2 as a potential target for therapeutic interventions that aim at modulating the p53 pathway to treat cancer.
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Specifications

Form

Liquid

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General info

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins, including p53/TP53, TP73, HDAC1 and CDKN1B (PubMed : 16914734, PubMed : 17721809, PubMed : 18006823, PubMed : 19043414, PubMed : 19483087, PubMed : 21994467). Mediates ubiquitination and degradation of p53/TP53; preferentially acts on tetrameric p53/TP53 (PubMed : 19043414, PubMed : 19483087). Catalyzes monoubiquitinates the translesion DNA polymerase POLH (PubMed : 21791603). Involved in the ribosome-associated quality control (RQC) pathway, which mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes : RCHY1 acts downstream of NEMF and recognizes CAT tails associated with stalled nascent chains, leading to their ubiquitination and degradation (PubMed : 33909987).. Isoform 4. Has no E3 ubiquitin-protein ligase activity.

Post-translational modifications

Subject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability.

Subcellular localisation

Nucleus

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Product protocols

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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins, including p53/TP53, TP73, HDAC1 and CDKN1B (PubMed : 16914734, PubMed : 17721809, PubMed : 18006823, PubMed : 19043414, PubMed : 19483087, PubMed : 21994467). Mediates ubiquitination and degradation of p53/TP53; preferentially acts on tetrameric p53/TP53 (PubMed : 19043414, PubMed : 19483087). Catalyzes monoubiquitinates the translesion DNA polymerase POLH (PubMed : 21791603). Involved in the ribosome-associated quality control (RQC) pathway, which mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes : RCHY1 acts downstream of NEMF and recognizes CAT tails associated with stalled nascent chains, leading to their ubiquitination and degradation (PubMed : 33909987).. Isoform 4. Has no E3 ubiquitin-protein ligase activity.
See full target information RCHY1
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Complementary Products

Primary Antibodies

AB189907

Anti-Pirh2 antibody [EPR18553]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Pirh2 antibody [EPR18553] (AB189907)

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Cell Lines & Lysates

AB258171

Human RCHY1 (Pirh2) knockout HeLa cell lysate

Western blot - Human RCHY1 (Pirh2) knockout HeLa cell lysate (AB258171)

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Cell Lines & Lysates

AB265478

Human RCHY1 (Pirh2) knockout HeLa cell line

Advanced Validation

Western blot - Human RCHY1 (Pirh2) knockout HeLa cell line (AB265478)

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