JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB123170

Recombinant Human Pirin/PIR protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant Human Pirin/PIR protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 290 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Pirin, Probable quercetinase, PIR

1 Images
SDS-PAGE - Recombinant Human Pirin/PIR protein (His tag N-Terminus) (AB123170)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Pirin/PIR protein (His tag N-Terminus) (AB123170)

15% SDS-PAGE showing ab123170 at approximately 34.3kDa (3µg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

O00625

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Previously labelled as Pirin.
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRSSEKMVEPQYQELKSEEIPKPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHHTAVLGEGDSVQVENKDPKRSHFVLIAGEPLREPVIQHGPFVMNTNEEISQAILDFRNAKNGFERAKTWKSKIGN","proteinLength":"Full Length","predictedMolecularWeight":"34.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":290,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00625","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pirin also known as PIR protein is a nuclear iron-dependent non-heme oxidoreductase involved in various cellular processes. The PIR protein sometimes called PIR-B or PIR-A/B has a molecular mass of approximately 32 kDa. It expresses in many human tissues including the brain heart and lungs. The PIR protein plays significant roles in stress response and regulation of transcription factors making it a subject of interest in cellular regulation and signal transduction research.
Biological function summary

PIR protein associates with transcriptional co-factors and mediates gene expression regulation impacting cellular metabolism and growth. It interacts with several alternative forms such as PIR alternative and antipirin contributing to its diverse functionality. PIR often forms part of transcription complexes facilitating the modulation of gene networks essential for maintaining cellular homeostasis.

Pathways

PIR protein participates in significant biological processes like the NF-kB signaling and apoptosis regulatory pathways. In the NF-kB pathway PIR contributes to transcription factor activation while in apoptosis it influences pathways promoting cell survival or programmed cell death. PIR protein relates closely to proteins such as Bcl-3 and related transcription factors shaping cellular responses to external stimuli and maintaining balance between cell survival and death.

PIR protein associates with various cancers and neurodegenerative diseases. In cancer PIR's role in transcription regulation impacts tumor progression and metastasis interacting with proteins like c-Myc. In neurodegeneration abnormalities in PIR expression or function link to disorders such as Alzheimer's disease where it interacts with tau protein exacerbating the disease pathology. Understanding PIR's relationship to these conditions might provide insights into new therapeutic approaches.
style
left-column

Specifications

Form

Liquid

Additional notes

ab123170 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro).

Sequence similarities

Belongs to the pirin family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro).
See full target information PIR
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:8923 PubMed39414818

2024

Streamlined analysis of drug targets by proteome integral solubility alteration indicates organ-specific engagement.

Applications

Unspecified application

Species

Unspecified reactive species

Tanveer Singh Batth,Marie Locard-Paulet,Nadezhda T Doncheva,Blanca Lopez Mendez,Lars Juhl Jensen,Jesper Velgaard Olsen
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB75858

Anti-CD99 antibody [EPR3097Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (AB75858)

  • 0
  • 0
Proteins & Peptides

AB186093

Recombinant Human CD99 protein

  • 0
  • 0
Primary Antibodies

AB157212

Anti-Pirin/PIR antibody [EPR11375(B)]

RabMAb|Recombinant

Western blot - Anti-Pirin/PIR antibody [EPR11375(B)] (AB157212)

  • 0
  • 0
Primary Antibodies

AB4729

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

  • 5
  • 85

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com