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AB101666

Recombinant Human PITPN protein

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(1 Publication)

Recombinant Human PITPN protein is a Human Full Length protein, in the 1 to 270 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PITPN, PITPNA, Phosphatidylinositol transfer protein alpha isoform, PI-TP-alpha, PtdIns transfer protein alpha, PtdInsTP alpha

1 Images
SDS-PAGE - Recombinant Human PITPN protein (AB101666)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PITPN protein (AB101666)

15% SDS-PAGE analysis of 3μg ab101666.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q00169

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMVLLKEYRVILPVSVDEYQVGQLYSVAEASKNETGGGEGVEVLVNEPYEKDGEKGQYTHKIYHLQSKVPTFVRMLAPEGALNIHEKAWNAYPYCRTVITNEYMKEDFLIKIETWHKPDLGTQENVHKLEPEAWKHVEAVYIDIADRSQVLSKDYKAEEDPAKFKSIKTGRGPLGPNWKQELVNQKDCPYMCAYKLVTVKFKWWGLQNKVENFIHKQERRLFTNFHRQLFCWLDKWVDLTMDDIRRMEEETKRQLDEMRQKDPVKGMTADD","proteinLength":"Full Length","predictedMolecularWeight":"33.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":270,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q00169","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PITPN also known as phosphatidylinositol transfer protein alpha (PITPα) plays an important role in lipid signaling by transferring phosphatidylinositol and phosphatidylcholine between membranes. PITPN is a 35 kDa protein expressed in various tissues with significant levels in the brain and liver. It carries out its mechanical function by binding lipids within its hydrophobic pocket facilitating their transport between cellular membranes which is important for maintaining lipid equilibrium.
Biological function summary

PITPN ensures proper signal transduction and intracellular trafficking. It forms part of a complex that regulates the transfer and distribution of phospholipids. This activity is important for membrane dynamics impacting cell communication and growth. Moreover PITPN enables the formation of phosphoinositides which are essential for activating important signaling pathways related to cellular functions.

Pathways

PITPN integrates into key phosphatidylinositol signaling pathways. These pathways are critical for producing phosphoinositide second messengers that modulate a variety of cell processes. For example PITPN collaborates with proteins such as phospholipase C which operates in inositol phosphate production. This cooperation allows for careful coordination of cellular responses such as cytoskeletal rearrangement and membrane trafficking.

Abnormal PITPN function links to neural and metabolic disorders. Mutations in PITPN can result in neurological issues such as neurodegenerative diseases due to disrupted lipid homeostasis in the brain. Furthermore PITPN's interaction with proteins like insulin receptor substrates relates to insulin resistance and diabetes development. These connections highlight PITPN's potential as a biomarker or therapeutic target for managing these conditions.
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Specifications

Form

Liquid

Additional notes

ab101666 was purified using conventional chromatography techniques.

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General info

Function

Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes (PubMed : 10531358, PubMed : 14962392, PubMed : 15522822, PubMed : 18636990, PubMed : 22822086). Shows a preference for PI and PC containing shorter saturated or monosaturated acyl chains at the sn-1 and sn-2 positions (PubMed : 15522822, PubMed : 22822086). Preference order for PC is C16 : 1 > C16 : 0 > C18 : 1 > C18 : 0 > C20 : 4 and for PI is C16 : 1 > C16 : 0 > C18 : 1 > C18 : 0 > C20 : 4 > C20 : 3 (PubMed : 22822086).

Sequence similarities

Belongs to the PtdIns transfer protein family. PI transfer class I subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes (PubMed : 10531358, PubMed : 14962392, PubMed : 15522822, PubMed : 18636990, PubMed : 22822086). Shows a preference for PI and PC containing shorter saturated or monosaturated acyl chains at the sn-1 and sn-2 positions (PubMed : 15522822, PubMed : 22822086). Preference order for PC is C16 : 1 > C16 : 0 > C18 : 1 > C18 : 0 > C20 : 4 and for PI is C16 : 1 > C16 : 0 > C18 : 1 > C18 : 0 > C20 : 4 > C20 : 3 (PubMed : 22822086).
See full target information PITPNA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 8: PubMed32046307

2020

A Novel Combined Scientific and Artistic Approach for the Advanced Characterization of Interactomes: The Akirin/Subolesin Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Artigas-Jerónimo,Juan J Pastor Comín,Margarita Villar,Marinela Contreras,Pilar Alberdi,Israel León Viera,Leandro Soto,Raúl Cordero,James J Valdés,Alejandro Cabezas-Cruz,Agustín Estrada-Peña,José de la Fuente
View all publications
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Secondary Antibodies

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Secondary Antibodies

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Secondary Antibodies

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