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AB276650

Recombinant Human PITPN protein (His tag)

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Recombinant Human PITPN protein (His tag) is a Human Full Length protein, in the 1 to 270 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

PITPN, PITPNA, Phosphatidylinositol transfer protein alpha isoform, PI-TP-alpha, PtdIns transfer protein alpha, PtdInsTP alpha

1 Images
SDS-PAGE - Recombinant Human PITPN protein (His tag) (AB276650)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PITPN protein (His tag) (AB276650)

SDS-PAGE analysis of ab276650

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q00169

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 8% Glycerol (glycerin, glycerine), 5% D-(+)-Trehalose dihydrate, 0.88% Sodium chloride, 0.24% Tris, 0.2% Tween 80

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Complementary Products

Proteins & Peptides

AB101091

Recombinant Human PITPNB protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human PITPNB protein (His tag N-Terminus) (AB101091)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB99695

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MVLLKEYRVILPVSVDEYQVGQLYSVAEASKNETGGGEGVEVLVNEPYEKDGEKGQYTHKIYHLQSKVPTFVRMLAPEGALNIHEKAWNAYPYCRTVITNEYMKEDFLIKIETWHKPDLGTQENVHKLEPEAWKHVEAVYIDIADRSQVLSKDYKAEEDPAKFKSIKTGRGPLGPNWKQELVNQKDCPYMCAYKLVTVKFKWWGLQNKVENFIHKQERRLFTNFHRQLFCWLDKWVDLTMDDIRRMEEETKRQLDEMRQKDPVKGMTADD","proteinLength":"Full Length","predictedMolecularWeight":"33.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":270,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q00169","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PITPN also known as phosphatidylinositol transfer protein alpha (PITPα) plays an important role in lipid signaling by transferring phosphatidylinositol and phosphatidylcholine between membranes. PITPN is a 35 kDa protein expressed in various tissues with significant levels in the brain and liver. It carries out its mechanical function by binding lipids within its hydrophobic pocket facilitating their transport between cellular membranes which is important for maintaining lipid equilibrium.
Biological function summary

PITPN ensures proper signal transduction and intracellular trafficking. It forms part of a complex that regulates the transfer and distribution of phospholipids. This activity is important for membrane dynamics impacting cell communication and growth. Moreover PITPN enables the formation of phosphoinositides which are essential for activating important signaling pathways related to cellular functions.

Pathways

PITPN integrates into key phosphatidylinositol signaling pathways. These pathways are critical for producing phosphoinositide second messengers that modulate a variety of cell processes. For example PITPN collaborates with proteins such as phospholipase C which operates in inositol phosphate production. This cooperation allows for careful coordination of cellular responses such as cytoskeletal rearrangement and membrane trafficking.

Abnormal PITPN function links to neural and metabolic disorders. Mutations in PITPN can result in neurological issues such as neurodegenerative diseases due to disrupted lipid homeostasis in the brain. Furthermore PITPN's interaction with proteins like insulin receptor substrates relates to insulin resistance and diabetes development. These connections highlight PITPN's potential as a biomarker or therapeutic target for managing these conditions.
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General info

Function

Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes (PubMed : 10531358, PubMed : 14962392, PubMed : 15522822, PubMed : 18636990, PubMed : 22822086). Shows a preference for PI and PC containing shorter saturated or monosaturated acyl chains at the sn-1 and sn-2 positions (PubMed : 15522822, PubMed : 22822086). Preference order for PC is C16 : 1 > C16 : 0 > C18 : 1 > C18 : 0 > C20 : 4 and for PI is C16 : 1 > C16 : 0 > C18 : 1 > C18 : 0 > C20 : 4 > C20 : 3 (PubMed : 22822086).

Sequence similarities

Belongs to the PtdIns transfer protein family. PI transfer class I subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes (PubMed : 10531358, PubMed : 14962392, PubMed : 15522822, PubMed : 18636990, PubMed : 22822086). Shows a preference for PI and PC containing shorter saturated or monosaturated acyl chains at the sn-1 and sn-2 positions (PubMed : 15522822, PubMed : 22822086). Preference order for PC is C16 : 1 > C16 : 0 > C18 : 1 > C18 : 0 > C20 : 4 and for PI is C16 : 1 > C16 : 0 > C18 : 1 > C18 : 0 > C20 : 4 > C20 : 3 (PubMed : 22822086).
See full target information PITPNA
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Product promise

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