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AB114744

Recombinant Human PITX1/BFT protein (GST tag N-Terminus)

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Recombinant Human PITX1/BFT protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 314 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

BFT, PTX1, PITX1, Pituitary homeobox 1, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, Paired-like homeodomain transcription factor 1

1 Images
SDS-PAGE - Recombinant Human PITX1/BFT protein (GST tag N-Terminus) (AB114744)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PITX1/BFT protein (GST tag N-Terminus) (AB114744)

12.5% SDS-PAGE showing ab114744 at approximately 60.61kDa.
Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P78337

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB70273

Anti-PITX1/BFT antibody

Immunoprecipitation - Anti-PITX1/BFT antibody (AB70273)

  • 0
  • 0
Primary Antibodies

AB174294

Anti-WDTC1 antibody [EPR12504]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (AB174294)

  • 0
  • 0
Primary Antibodies

AB229338

Anti-Lyl1 antibody - C-terminal

Western blot - Anti-Lyl1 antibody - C-terminal (AB229338)

  • 0
  • 0
Primary Antibodies

AB228006

Anti-XAB2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XAB2 antibody - N-terminal (AB228006)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein)</p>" } } }
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Product details

This product was previously labelled as PITX1.
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Sequence info

[{"sequence":"MDAFKGGMSLERLPEGLRPPPPPPHDMGPAFHLARPADPREPLENSASESSDTELPEKERGGEPKGPEDSGAGGTGCGGADDPAKKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSMREEIAVWTNLTEPRVRVWFKNRRAKWRKRERNQQLDLCKGGYVPQFSGLVQPYEDVYAAGYSYNNWAAKSLAPAPLSTKSFTFFNSMSPLSSQSMFSAPSSISSMTMPSSMGPGAVPGMPNSGLNNINNLTGSSLNSAMSPGACPYGTPASPYSVYRDTCNSSLASLRLKSKQHSSFGYGALQGPASGLNACQYNS","proteinLength":"Full Length","predictedMolecularWeight":"60.61 kDa","actualMolecularWeight":null,"aminoAcidEnd":314,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P78337","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PITX1 gene also known as BFT or BFT NZ encodes the Pituitary Homeobox 1 protein. This protein is a homeodomain transcription factor and plays an important role in regulating gene expression by binding to specific DNA sequences. PITX1 has a molecular mass of approximately 33 kDa. It is primarily expressed in tissues like the pituitary gland lower limbs and jaw. The expression in these specific areas hints at its importance in the developmental processes that occur during embryogenesis.
Biological function summary

The PITX1 protein functions as part of the regulation of limb and craniofacial development. It is not part of a larger protein complex but works independently to influence the differentiation and development of specific cell types. In the embryonic stages PITX1 directs the growth and formation of lower limb structures and mutations in this gene can lead to limb malformations. The protein also has roles in craniofacial development highlighting its multifaceted biological functions.

Pathways

PITX1 is involved in the Wnt signaling pathway and the Hedgehog pathway both essential for proper developmental processes. In the Wnt signaling pathway PITX1 interacts with other proteins such as β-catenin to modulate gene expression necessary for limb development. In the Hedgehog pathway PITX1 influences how cells respond to Hedgehog signals affecting cell growth and specialization. The coordinated role of PITX1 in these pathways supports its critical involvement in developmental regulation.

PITX1 has connections to clubfoot and Liebenberg syndrome. Clubfoot a congenital deformity of the foot often links to mutations or alterations in PITX1 expression affecting limb development. Moreover Liebenberg syndrome suggests altered expression or function of PITX1 leading to misregulated limb and joint formation. In both diseases associations with proteins like β-catenin and hedgehog proteins illustrate how changes in PITX1 can result in significant phenotypic abnormalities reflecting its importance in both normal and pathological conditions.
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Specifications

Form

Liquid

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General info

Function

Sequence-specific transcription factor that binds gene promoters and activates their transcription. May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.

Sequence similarities

Belongs to the paired homeobox family. Bicoid subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Sequence-specific transcription factor that binds gene promoters and activates their transcription. May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
See full target information PITX1
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Product promise

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