Recombinant Human PITX1/BFT protein is a Human Full Length protein, in the 1 to 314 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M D A F K G G M S L E R L P E G L R P P P P P P H D M G P A F H L A R P A D P R E P L E N S A S E S S D T E L P E K E R G G E P K G P E D S G A G G T G C G G A D D P A K K K K Q R R Q R T H F T S Q Q L Q E L E A T F Q R N R Y P D M S M R E E I A V W T N L T E P R V R V W F K N R R A K W R K R E R N Q Q L D L C K G G Y V P Q F S G L V Q P Y E D V Y A A G Y S Y N N W A A K S L A P A P L S T K S F T F F N S M S P L S S Q S M F S A P S S I S S M T M P S S M G P G A V P G M P N S G L N N I N N L T G S S L N S A M S P G A C P Y G T P A S P Y S V Y R D T C N S S L A S L R L K S K Q H S S F G Y G A L Q G P A S G L N A C Q Y N S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes (Recombinant protein) |
Target data
Function
Sequence-specific transcription factor that binds gene promoters and activates their transcription. May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
Alternative names
BFT, PTX1, PITX1, Pituitary homeobox 1, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, Paired-like homeodomain transcription factor 1
Recommended products
Recombinant Human PITX1/BFT protein is a Human Full Length protein, in the 1 to 314 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P78337-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M D A F K G G M S L E R L P E G L R P P P P P P H D M G P A F H L A R P A D P R E P L E N S A S E S S D T E L P E K E R G G E P K G P E D S G A G G T G C G G A D D P A K K K K Q R R Q R T H F T S Q Q L Q E L E A T F Q R N R Y P D M S M R E E I A V W T N L T E P R V R V W F K N R R A K W R K R E R N Q Q L D L C K G G Y V P Q F S G L V Q P Y E D V Y A A G Y S Y N N W A A K S L A P A P L S T K S F T F F N S M S P L S S Q S M F S A P S S I S S M T M P S S M G P G A V P G M P N S G L N N I N N L T G S S L N S A M S P G A C P Y G T P A S P Y S V Y R D T C N S S L A S L R L K S K Q H S S F G Y G A L Q G P A S G L N A C Q Y N S
- Accession
- P78337
- Protein length
- Full Length
- Predicted molecular weight
- 60.61 kDa
- Amino acids
- 1 to 314
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Sequence-specific transcription factor that binds gene promoters and activates their transcription. May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
- Sequence similarities
Belongs to the paired homeobox family. Bicoid subfamily.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as PITX1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PITX1 gene also known as BFT or BFT NZ encodes the Pituitary Homeobox 1 protein. This protein is a homeodomain transcription factor and plays an important role in regulating gene expression by binding to specific DNA sequences. PITX1 has a molecular mass of approximately 33 kDa. It is primarily expressed in tissues like the pituitary gland lower limbs and jaw. The expression in these specific areas hints at its importance in the developmental processes that occur during embryogenesis.
- Biological function summary
The PITX1 protein functions as part of the regulation of limb and craniofacial development. It is not part of a larger protein complex but works independently to influence the differentiation and development of specific cell types. In the embryonic stages PITX1 directs the growth and formation of lower limb structures and mutations in this gene can lead to limb malformations. The protein also has roles in craniofacial development highlighting its multifaceted biological functions.
- Pathways
PITX1 is involved in the Wnt signaling pathway and the Hedgehog pathway both essential for proper developmental processes. In the Wnt signaling pathway PITX1 interacts with other proteins such as β-catenin to modulate gene expression necessary for limb development. In the Hedgehog pathway PITX1 influences how cells respond to Hedgehog signals affecting cell growth and specialization. The coordinated role of PITX1 in these pathways supports its critical involvement in developmental regulation.
- Associated diseases and disorders
PITX1 has connections to clubfoot and Liebenberg syndrome. Clubfoot a congenital deformity of the foot often links to mutations or alterations in PITX1 expression affecting limb development. Moreover Liebenberg syndrome suggests altered expression or function of PITX1 leading to misregulated limb and joint formation. In both diseases associations with proteins like β-catenin and hedgehog proteins illustrate how changes in PITX1 can result in significant phenotypic abnormalities reflecting its importance in both normal and pathological conditions.
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1 product image
SDS-PAGE - Recombinant Human PITX1/BFT protein (ab114744)
12.5% SDS-PAGE showing ab114744 at approximately 60.61kDa.
Stained with Coomassie Blue.
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Product protocols
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