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AB60837

Recombinant human PKA beta (catalytic subunit) protein

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Recombinant human PKA beta (catalytic subunit) protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS, WB.

View Alternative Names

cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACB

5 Images
Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (AB60837)
  • FuncS

Unknown

Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

The specific activity of PKA beta (catalytic subunit) (ab60837) was determined to be 430 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (AB60837)
  • FuncS

Unknown

Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

Sample Kinase Activity Plot.

Western blot - Recombinant human PKA beta (catalytic subunit) protein (AB60837)
  • WB

Unknown

Western blot - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

All lanes:

Anti-PKA beta (catalytic subunit) antibody (<a href='/en-us/products/unavailable/pka-beta-catalytic-subunit-antibody-ab94612'>ab94612</a>) at 1 µg/mL

Lane 1:

Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837) at 0.1 µg

Lane 2:

Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 1min

SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (AB60837)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

ab60837 on SDS-PAGE, MW ~65 kDa.

SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (AB60837)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

SDS PAGE analysis of ab60837

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, WB, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Active

Accession

P22694

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB116027

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)

  • 5
  • 2
Primary Antibodies

AB75991

Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (AB75991)

  • 5
  • 3
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB187515

Anti-PKA beta (catalytic subunit) antibody

Immunocytochemistry/ Immunofluorescence - Anti-PKA beta (catalytic subunit) antibody (AB187515)

  • 0
  • 0
Proteins & Peptides

AB285976

Recombinant EN-TEV Protease protein

Bioactive

Size Exclusion Chromatography - Recombinant EN-TEV Protease protein (AB285976)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab60837 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/pka-beta-catalytic-subunit-antibody-ab94612'>ab94612</a>.</p>" } } }
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Product details

ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"65 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P22694","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PKA beta (catalytic subunit) also known as PRKACB or Protein Kinase A catalytic subunit beta plays a pivotal role in cellular signaling mechanisms. Mechanically it catalyzes the transfer of a phosphate group from ATP to a serine or threonine residue in target proteins impacting various cellular processes. This catalytic subunit has a molecular weight of approximately 40 kDa. It expresses widely in many tissues including the brain heart and skeletal muscle where it contributes to regulating metabolic and transcriptional activities.
Biological function summary

The PKA beta catalytic subunit functions in a larger context as part of the Protein Kinase A (PKA) complex which is a central player in the cyclic AMP (cAMP) signaling pathway. This complex acts as an effector of cAMP translating extracellular signals into intracellular actions by phosphorylating a wide range of target proteins. As a part of this complex it modulates activities related to glucose metabolism cell proliferation and gene expression reflecting its involvement in many cellular processes.

Pathways

PKA beta is integrally involved in the cAMP signaling and insulin signaling pathways. In the cAMP pathway it activates by binding to cAMP leading to dissociation from regulatory subunits and subsequent activation of substrate proteins. In the insulin signaling pathway it interacts with proteins like PI3K and Akt playing an important role in regulating glucose homeostasis and lipid metabolism. These pathways illustrate the PKA beta's importance in maintaining cellular and systemic metabolic balance.

The dysfunction of PKA beta is associated with conditions such as cardiac hypertrophy and certain types of cancer. Elevations or disruptions in PKA beta activity can lead to alterations in cell growth and metabolic regulation. PKA beta's relationship with proteins like CREB and Bcl-2 through these disorders has been observed where aberrant phosphorylation mediated by PKA can drive pathological cell survival proliferation and growth linking malfunction directly to disease progression.
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Specifications

Form

Liquid

Additional notes

Purity: >90% as determined by densitometry. Affinity purified.

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General info

Function

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs (PubMed : 12420224, PubMed : 21423175, PubMed : 31112131). PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed : 12420224, PubMed : 21423175). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PubMed : 12420224, PubMed : 21423175). Phosphorylates GPKOW which regulates its ability to bind RNA (PubMed : 21880142). Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR (PubMed : 31112131).

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.

Post-translational modifications

Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.

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Product protocols

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Target data

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs (PubMed : 12420224, PubMed : 21423175, PubMed : 31112131). PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed : 12420224, PubMed : 21423175). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PubMed : 12420224, PubMed : 21423175). Phosphorylates GPKOW which regulates its ability to bind RNA (PubMed : 21880142). Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR (PubMed : 31112131).
See full target information PRKACB
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