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AB132177

Recombinant Human PKA R2/PKR2 protein (GST tag N-Terminus)

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Recombinant Human PKA R2/PKR2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 382 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

PKR2, PRKAR2, PRKAR2A, cAMP-dependent protein kinase type II-alpha regulatory subunit

1 Images
SDS-PAGE - Recombinant Human PKA R2/PKR2 protein (GST tag N-Terminus) (AB132177)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PKA R2/PKR2 protein (GST tag N-Terminus) (AB132177)

12.5% SDS-PAGE analysis of ab132177 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

P13861

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB32390

Anti-PKA R2/PKR2 (phospho S99) antibody [E151]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (AB32390)

  • 4
  • 1
Primary Antibodies

AB75991

Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (AB75991)

  • 5
  • 3
Primary Antibodies

AB32096

Anti-CREB (phospho S133) antibody [E113]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody [E113] (AB32096)

  • 4
  • 14
Cell Lines & Lysates

AB265748

Human PRKAR2A (PKA R2/PKR2) knockout HeLa cell line

Advanced Validation

Western blot - Human PRKAR2A (PKA R2/PKR2) knockout HeLa cell line (AB265748)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PKA R2.
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Sequence info

[{"sequence":"MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ","proteinLength":"Full Length","predictedMolecularWeight":"69.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":382,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P13861","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein Kinase A R2 (PKR2) also known as PRKAR2A is a regulatory subunit of Protein Kinase A (PKA). PKA is part of a larger complex known as the cAMP-dependent protein kinase which plays an important role in cellular signaling. PKA consists of regulatory and catalytic subunits with PKR2 serving as one of its regulatory forms. PKR2 possesses a molecular mass of approximately 47 kDa and is available in various human tissues with notable expression in the brain and cardiovascular system.
Biological function summary

PKR2 regulates the activity of PKA by binding to the catalytic subunit in the absence of cAMP therefore inhibiting its activity. When cAMP is present it binds to the regulatory subunit PKR2 leading to the release and activation of the catalytic subunit of PKA. PKR2 is an integral part of the PKA holoenzyme complex which plays a critical role in mediating cellular responses to hormonal stimulation.

Pathways

PKR2 is heavily involved in the cAMP-dependent signaling pathway. This pathway is essential for translating extracellular signals into cellular actions. It interacts with various effector proteins including adenylate cyclases and phosphodiesterases to modulate responses such as gene transcription metabolism and memory formation. PKR2-related activity influences pathways shared with proteins like the G-protein subunits which facilitate the effects of neurotransmitters.

PKR2 irregularities can impact conditions like heart failure and neurodegenerative diseases. Disrupted PKA pathway signaling involving PKR2 can contribute to cardiac dysfunction by affecting heart muscle contractility and hypertrophy. In neurodegenerative diseases the improper functioning of PKR2 can exacerbate disruptions in neural signaling. A connection exists between PKR2 and proteins such as CREB (cAMP response element-binding protein) which links PKR2's role to memory deficits observed in neurodegeneration.
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Specifications

Form

Liquid

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General info

Function

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Sequence similarities

Belongs to the cAMP-dependent kinase regulatory chain family.

Post-translational modifications

Phosphorylated by the activated catalytic chain.

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Product protocols

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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
See full target information PRKAR2A
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Product promise

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