Recombinant Human PKA R2/PKR2 protein is a Human Full Length protein, in the 1 to 382 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Amino acid sequence
M S H I Q I P P G L T E L L Q G Y T V E V L R Q Q P P D L V E F A V E Y F T R L R E A R A P A S V L P A A T P R Q S L G H P P P E P G P D R V A D A K G D S E S E E D E D L E V P V P S R F N R R V S V C A E T Y N P D E E E E D T D P R V I H P K T D E Q R C R L Q E A C K D I L L F K N L D Q E Q L S Q V L D A M F E R I V K A D E H V I D Q G D D G D N F Y V I E R G T Y D I L V T K D N Q T R S V G Q Y D N R G S F G E L A L M Y N T P R A A T I V A T S E G S L W G L D R V T F R R I I V K N N A K K R K M F E S F I E S V P L L K S L E V S E R M K I V D V I G E K I Y K D G E R I I T Q T K S N K D G G N Q E V E I A R C H K G Q Y F G E L A L V T N K P R A A S A Y A V G D V K C L V M D V Q A F E R L L G P C M D I M K R N I S H Y E E Q L V K M F G S S V D L G N L G Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Alternative names
cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2, PKR2, PRKAR2A
Recommended products
Recombinant Human PKA R2/PKR2 protein is a Human Full Length protein, in the 1 to 382 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Alternative names
cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2, PKR2, PRKAR2A
Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Accession
- P13861-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
M S H I Q I P P G L T E L L Q G Y T V E V L R Q Q P P D L V E F A V E Y F T R L R E A R A P A S V L P A A T P R Q S L G H P P P E P G P D R V A D A K G D S E S E E D E D L E V P V P S R F N R R V S V C A E T Y N P D E E E E D T D P R V I H P K T D E Q R C R L Q E A C K D I L L F K N L D Q E Q L S Q V L D A M F E R I V K A D E H V I D Q G D D G D N F Y V I E R G T Y D I L V T K D N Q T R S V G Q Y D N R G S F G E L A L M Y N T P R A A T I V A T S E G S L W G L D R V T F R R I I V K N N A K K R K M F E S F I E S V P L L K S L E V S E R M K I V D V I G E K I Y K D G E R I I T Q T K S N K D G G N Q E V E I A R C H K G Q Y F G E L A L V T N K P R A A S A Y A V G D V K C L V M D V Q A F E R L L G P C M D I M K R N I S H Y E E Q L V K M F G S S V D L G N L G Q
- Accession
- Protein length
Full Length
- Predicted molecular weight
69.5 kDa
- Amino acids
1 to 382
- Nature
Recombinant
Specifications
- Form
Liquid
General info
- Function
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
- Sequence similarities
Belongs to the cAMP-dependent kinase regulatory chain family.
- Post-translational modifications
Phosphorylated by the activated catalytic chain.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product was previously labelled as PKA R2.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein Kinase A R2 (PKR2) also known as PRKAR2A is a regulatory subunit of Protein Kinase A (PKA). PKA is part of a larger complex known as the cAMP-dependent protein kinase which plays an important role in cellular signaling. PKA consists of regulatory and catalytic subunits with PKR2 serving as one of its regulatory forms. PKR2 possesses a molecular mass of approximately 47 kDa and is available in various human tissues with notable expression in the brain and cardiovascular system.
- Biological function summary
PKR2 regulates the activity of PKA by binding to the catalytic subunit in the absence of cAMP therefore inhibiting its activity. When cAMP is present it binds to the regulatory subunit PKR2 leading to the release and activation of the catalytic subunit of PKA. PKR2 is an integral part of the PKA holoenzyme complex which plays a critical role in mediating cellular responses to hormonal stimulation.
- Pathways
PKR2 is heavily involved in the cAMP-dependent signaling pathway. This pathway is essential for translating extracellular signals into cellular actions. It interacts with various effector proteins including adenylate cyclases and phosphodiesterases to modulate responses such as gene transcription metabolism and memory formation. PKR2-related activity influences pathways shared with proteins like the G-protein subunits which facilitate the effects of neurotransmitters.
- Associated diseases and disorders
PKR2 irregularities can impact conditions like heart failure and neurodegenerative diseases. Disrupted PKA pathway signaling involving PKR2 can contribute to cardiac dysfunction by affecting heart muscle contractility and hypertrophy. In neurodegenerative diseases the improper functioning of PKR2 can exacerbate disruptions in neural signaling. A connection exists between PKR2 and proteins such as CREB (cAMP response element-binding protein) which links PKR2's role to memory deficits observed in neurodegeneration.
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1 product image
SDS-PAGE - Recombinant Human PKA R2/PKR2 protein (ab132177)
12.5% SDS-PAGE analysis of ab132177 stained with Coomassie Blue.
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