AB55672

Recombinant human PKC alpha protein (Active)

Name: Recombinant human PKC alpha protein (Active) (ab55672) | Abcam
Brand: Abcam Limited
SKU: AB55672
Price: 275 USD
Availability: InStock

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(6 Publications)

Recombinant human PKC alpha protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS, WB.

View Alternative Names

PKCA, PRKACA, PRKCA, Protein kinase C alpha type, PKC-A, PKC-alpha

14 Images

Lab

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Blocking and dilution buffer : 5% NFDM/TBST. Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag. Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag. Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag. Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag. Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag. Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag. Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag. Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.

All lanes:

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (<a href='/en-us/products/primary-antibodies/pkc-phospho-s660-antibody-ep1902y-ab75837'>ab75837</a>) at 1/20000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 5:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 8:

Western blot - Recombinant human PKC mu/PKD protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 100-150 kDa

false

Exposure time: 1s

Lab

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Blocking and dilution buffer : 5% NFDM/TBST.

ab109539 could detect PKC gamma (pT514), PKC alpha (pT497), PKC beta (pT500), PKC delta (pT507), PKC eta (pT513), PKC epsilon (pT566) and PKC theta (pT538).

Active human PKC alpha full length protein (Catalog#ab55672) contains aa1-672 with GST-tag;
Active human PKC beta 1 full length protein (Catalog#ab60840) contains aa1-671 with GST-tag;
Active human PKC beta 2 full length protein (Catalog#ab60841) contains aa1-673 with GST-tag;
Active human PKC delta full length protein (Catalog#ab60844) contains aa1-676 with GST-tag;
Active human PKC eta full length protein (Catalog#ab60849) contains aa1-683 with GST-tag;
Active human PKC epsilon full length protein (Catalog#ab60847) contains aa1-737 with GST-tag;
Active human PKC theta full length protein (Catalog#ab56641) contains aa1-706 with GST-tag;
Active human PKC mu full length protein (Catalog#ab60873) contains aa1-912 with GST-tag;
Active human PKC gamma full length protein (Catalog#ab60842) contains aa1-697 with GST-tag.

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (<a href='/en-us/products/primary-antibodies/pkc-phospho-t514-antibody-ep2730y-ab109539'>ab109539</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672) at 0.5 µg

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>) at 0.2 µg

Lane 3:

Active human PKC beta 2 full length protein at 0.2 µg

Lane 4:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>) at 0.05 µg

Lane 5:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>) at 0.5 µg

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>) at 1 µg

Lane 7:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>) at 0.2 µg

Lane 8:

Western blot - Recombinant human PKC mu/PKD protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>) at 1 µg

Lane 9:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 77 kDa

Observed band size: 80 kDa

false

Exposure time: 180s

Functional Studies - Recombinant human PKC alpha protein (Active) (AB55672)

FuncS

Supplier Data

Functional Studies - Recombinant human PKC alpha protein (Active) (AB55672)

Specific activity : 3,200 nmol/min/mg

Lab

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

5% NFDM/TBST

All lanes:

Western blot - Anti-PKC alpha antibody [133] (<a href='/en-us/products/primary-antibodies/pkc-alpha-antibody-133-ab11723'>ab11723</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672)

Lane 2:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 76 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

5% NFDM/TBST All positive controls 10 ng

All lanes:

Western blot - Anti-PKC alpha antibody [133] (<a href='/en-us/products/primary-antibodies/pkc-alpha-antibody-133-ab11723'>ab11723</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 5:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 6:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 7:

Western blot - Recombinant human PKC mu/PKD protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>)

Lane 8:

Western blot - Recombinant human PKC zeta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 9:

Western blot - Recombinant human PKC iota protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Lane 10:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 76 kDa

false

Supplier Data

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

This data was developed using ab180848, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (<a href='/en-us/products/primary-antibodies/pkc-alpha-phospho-s657-antibody-epr19012-ab180848'>ab180848</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 8:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 76 kDa

Observed band size: 105 kDa

false

Exposure time: 60s

Unknown

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

All lanes:

Anti-PKC alpha antibody (<a href='/en-us/products/unavailable/pkc-alpha-antibody-ab66181'>ab66181</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 10s

Supplier Data

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32376).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC alpha antibody [Y124] (<a href='/en-us/products/primary-antibodies/pkc-alpha-antibody-y124-ab32376'>ab32376</a>) at 1/2000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 8:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 76 kDa

Observed band size: 105 kDa

false

Exposure time: 20s

Supplier Data

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

This data was developed using ab76129, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1-4 : 180 seconds; Lane 5-6 : 60 seconds; Lane 7-10 : 5 seconds.

All lanes:

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (<a href='/en-us/products/primary-antibodies/pkc-zeta-phospho-t410-pkc-iota-phospho-t412-antibody-ep1491y-ab76129'>ab76129</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 8:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa

false

Supplier Data

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

This data was developed using ab32502, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC alpha (phospho T638) antibody [E195] (<a href='/en-us/products/primary-antibodies/pkc-alpha-phospho-t638-antibody-e195-ab32502'>ab32502</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC zeta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 7:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 8:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 9:

Western blot - Recombinant human PKC iota protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 76 kDa

Observed band size: 105 kDa

false

Exposure time: 60s

SDS-PAGE - Recombinant human PKC alpha protein (Active) (AB55672)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PKC alpha protein (Active) (AB55672)

Purity >95% by densiometry.

Supplier Data

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62372).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (<a href='/en-us/products/primary-antibodies/pkc-zeta-phospho-t560-antibody-ep2037ay-ab62372'>ab62372</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 8:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 105 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

This data was developed using ab53295, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC delta (phospho T507) antibody [EPR1485Y] (<a href='/en-us/products/primary-antibodies/pkc-delta-phospho-t507-antibody-epr1485y-ab53295'>ab53295</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC zeta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 7:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 8:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 9:

Western blot - Recombinant human PKC iota protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 105 kDa

false

Exposure time: 180s

Lab

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab235384 was used to detect GST tag.

All lanes:

Western blot - Anti-PKC beta 2 antibody [Y125] (<a href='/en-us/products/primary-antibodies/pkc-beta-2-antibody-y125-ab32026'>ab32026</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>) at 10 ng

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>) at 10 ng

Lane 3:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>) at 10 ng

Lane 4:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>) at 10 ng

Lane 5:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>) at 10 ng

Lane 6:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>) at 10 ng

Lane 7:

Western blot - Recombinant human PKC mu/PKD protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>) at 10 ng

Lane 8:

Western blot - Recombinant human PKC alpha protein (Active) (ab55672) at 10 ng

Lane 9:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 100 kDa

false

Key facts

Purity

>95% Densitometry

Expression system

Baculovirus infected Sf9 cells

Applications

WB, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: 3,200 nmol/min/mg.

Accession

P17252

Animal free

Carrier free

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab55672 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/pkc-alpha-antibody-ab66181'>ab66181</a>." } } }

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Product details

ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity.

For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.

Avoid repeated handling.

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Sequence info

[{"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"103 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P17252","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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Properties and storage information

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

True

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PKC alpha known as Protein Kinase C alpha is a serine/threonine-specific protein kinase involved in various cellular processes. The molecular mass of PKC alpha is approximately 80 kDa. Alternately called PKCA or PRKC alpha it is expressed in many tissues with high expression in the brain heart and lungs. PKC alpha plays a role in signal transduction by phosphorylating serine and threonine residues on target proteins.

Biological function summary

PKC alpha regulates cellular functions including proliferation differentiation and apoptosis. It often acts as part of a larger protein complex modulating various biological responses by interacting with substrates and co-factors. Its activity is dependent on calcium ions and diacylglycerol emphasizing its role in calcium and lipid signaling pathways.

Pathways

PKC alpha plays a vital role in the MAPK/ERK pathway and the PI3K/AKT pathway which are critical for the cellular stress response and growth regulation. PKC alpha interacts with several proteins in these pathways including RAF1 and AKT1 facilitating signal transmission that influences cell fate decisions.

PKC alpha has been implicated in cancer and cardiac hypertrophy. In cancers aberrant PKC alpha signaling is linked to increased cell proliferation and survival. In cardiac hypertrophy it is associated with abnormal heart growth due to impaired signaling involving AKT1 and GSK3B. These connections highlight PKC alpha's influence in pathological as well as physiological conditions.

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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. During chemokine-induced CD4(+) T cell migration, phosphorylates CDC42-guanine exchange factor DOCK8 resulting in its dissociation from LRCH1 and the activation of GTPase CDC42 (PubMed : 28028151). Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. Phosphorylates SOCS2 at 'Ser-52' facilitating its ubiquitination and proteasomal degradation (By similarity). Phosphorylates KLHL3 in response to angiotensin II signaling, decreasing the interaction between KLHL3 and WNK4 (PubMed : 25313067). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231).

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Post-translational modifications

In response to growth factors, phosphorylated at Thr-631 and Ser-657 by the mTORC2 complex, promoting autophosphorylation and activation of PRKCA.

Subcellular localisation

Mitochondrion membrane

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PRKCA

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:3956-3971 PubMed39113702

2024

PKCα Induced the Generation of Extracellular Vesicles in Activated Platelets to Promote Breast Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghua Zhao,Huan Tian,Xiaona Zhao,Lan Lan,Huanhuan Liu,Yi Sun,Fengyan Yu

Scientific reports 13:14844 PubMed37684289

2023

Valproate regulates inositol synthesis by reducing expression of myo-inositol-3-phosphate synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Kendall C Case,Rachel J Beltman,Mary Kay H Pflum,Miriam L Greenberg

eLife 10: PubMed34821217

2021

Reversible phosphorylation of cyclin T1 promotes assembly and stability of P-TEFb.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Huang,Trang Tt Nguyen,Ignacia Echeverria,Ramachandran Rakesh,Daniele C Cary,Hana Paculova,Andrej Sali,Arthur Weiss,Boris Matija Peterlin,Koh Fujinaga

Analytical biochemistry 608:113892 PubMed32810472

2020

Electrochemical detection of neuronal extracellular phosphorylation by PKA, PKC and Src.

Applications

Unspecified application

Species

Unspecified reactive species

S Ahmad,M N Hossain,S Ahmadi,K Kerman,H-B Kraatz

JCI insight 4: PubMed31578312

2019

KIAA0317 regulates pulmonary inflammation through SOCS2 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Travis B Lear,Alison C McKelvey,John W Evankovich,Shristi Rajbhandari,Tiffany A Coon,Sarah R Dunn,James D Londino,Bryan J McVerry,Yingze Zhang,Eleanor Valenzi,Christine L Burton,Rachael Gordon,Sebastien Gingras,Karina C Lockwood,Michael J Jurczak,Robert Lafyatis,Mark J Shlomchik,Yuan Liu,Bill B Chen

Analytical biochemistry 574:46-56 PubMed30914244

2019

Electrochemical studies of human nAChR a7 subunit phosphorylation by kinases PKA, PKC and Src.

Applications

Unspecified application

Species

Unspecified reactive species

Syed Ahmad,Zhe She,Heinz-Bernhard Kraatz

View all publications

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Product promise

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Recombinant human PKC alpha protein (Active)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Functional Studies - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

SDS-PAGE - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Western blot - Recombinant human PKC alpha protein (Active) (AB55672)

Key facts

Purity

Expression system

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Storage buffer

Complementary Products

Proteins & Peptides

Proteins & Peptides

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Post-translational modifications

Subcellular localisation

Product protocols

Target data

Publications (6)

Product promise