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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Recombinant human PKC alpha protein (Active) is a Human Full Length protein, in the 1 to 672 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS, WB.
>95% Densitometry
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS, WB
Yes
M A D V F P G N D S T A S Q D V A N R F A R K G A L R Q K N V H E V K D H K F I A R F F K Q P T F C S H C T D F I W G F G K Q G F Q C Q V C C F V V H K R C H E F V T F S C P G A D K G P D T D D P R S K H K F K I H T Y G S P T F C D H C G S L L Y G L I H Q G M K C D T C D M N V H K Q C V I N V P S L C G M D H T E K R G R I Y L K A E V A D E K L H V T V R D A K N L I P M D P N G L S D P Y V K L K L I P D P K N E S K Q K T K T I R S T L N P Q W N E S F T F K L K P S D K D R R L S V E I W D W D R T T R N D F M G S L S F G V S E L M K M P A S G W Y K L L N Q E E G E Y Y N V P I P E G D E E G N M E L R Q K F E K A K L G P A G N K V I S P S E D R K Q P S N N L D R V K L T D F N F L M V L G K G S F G K V M L A D R K G T E E L Y A I K I L K K D V V I Q D D D V E C T M V E K R V L A L L D K P P F L T Q L H S C F Q T V D R L Y F V M E Y V N G G D L M Y H I Q Q V G K F K E P Q A V F Y A A E I S I G L F F L H K R G I I Y R D L K L D N V M L D S E G H I K I A D F G M C K E H M M D G V T T R T F C G T P D Y I A P E I I A Y Q P Y G K S V D W W A Y G V L L Y E M L A G Q P P F D G E D E D E L F Q S I M E H N V S Y P K S L S K E A V S V C K G L M T K H P A K R L G C G P E G E R D V R E H A F F R R I D W E K L E N R E I Q P P F K P K V C G K G A E N F D K F F T R G Q P V L T P P D Q L V I A N I D Q S D F E G F S Y V N P Q F V H P I L Q S A V
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Ab55672 can be used as a WB positive control in conjunction with ab66181. |
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. During chemokine-induced CD4(+) T cell migration, phosphorylates CDC42-guanine exchange factor DOCK8 resulting in its dissociation from LRCH1 and the activation of GTPase CDC42 (PubMed:28028151). Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. Phosphorylates SOCS2 at 'Ser-52' facilitating its ubiquitination and proteosomal degradation (By similarity).
Protein kinase C alpha type, PKC-A, PKC-alpha, PKCA, PRKACA, PRKCA
Recombinant human PKC alpha protein (Active) is a Human Full Length protein, in the 1 to 672 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS, WB.
Protein kinase C alpha type, PKC-A, PKC-alpha, PKCA, PRKACA, PRKCA
>95% Densitometry
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS, WB
Yes
Specific Activity: 3,200 nmol/min/mg.
No
Human
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF
M A D V F P G N D S T A S Q D V A N R F A R K G A L R Q K N V H E V K D H K F I A R F F K Q P T F C S H C T D F I W G F G K Q G F Q C Q V C C F V V H K R C H E F V T F S C P G A D K G P D T D D P R S K H K F K I H T Y G S P T F C D H C G S L L Y G L I H Q G M K C D T C D M N V H K Q C V I N V P S L C G M D H T E K R G R I Y L K A E V A D E K L H V T V R D A K N L I P M D P N G L S D P Y V K L K L I P D P K N E S K Q K T K T I R S T L N P Q W N E S F T F K L K P S D K D R R L S V E I W D W D R T T R N D F M G S L S F G V S E L M K M P A S G W Y K L L N Q E E G E Y Y N V P I P E G D E E G N M E L R Q K F E K A K L G P A G N K V I S P S E D R K Q P S N N L D R V K L T D F N F L M V L G K G S F G K V M L A D R K G T E E L Y A I K I L K K D V V I Q D D D V E C T M V E K R V L A L L D K P P F L T Q L H S C F Q T V D R L Y F V M E Y V N G G D L M Y H I Q Q V G K F K E P Q A V F Y A A E I S I G L F F L H K R G I I Y R D L K L D N V M L D S E G H I K I A D F G M C K E H M M D G V T T R T F C G T P D Y I A P E I I A Y Q P Y G K S V D W W A Y G V L L Y E M L A G Q P P F D G E D E D E L F Q S I M E H N V S Y P K S L S K E A V S V C K G L M T K H P A K R L G C G P E G E R D V R E H A F F R R I D W E K L E N R E I Q P P F K P K V C G K G A E N F D K F F T R G Q P V L T P P D Q L V I A N I D Q S D F E G F S Y V N P Q F V H P I L Q S A V
Full Length
103 kDa
1 to 672
Recombinant
GST tag N-Terminus
Liquid
Affinity purified.
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. During chemokine-induced CD4(+) T cell migration, phosphorylates CDC42-guanine exchange factor DOCK8 resulting in its dissociation from LRCH1 and the activation of GTPase CDC42 (PubMed:28028151). Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. Phosphorylates SOCS2 at 'Ser-52' facilitating its ubiquitination and proteosomal degradation (By similarity).
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Mitochondrion membrane, Nucleus
Dry Ice
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Avoid repeated handling.
PKC alpha regulates cellular functions including proliferation differentiation and apoptosis. It often acts as part of a larger protein complex modulating various biological responses by interacting with substrates and co-factors. Its activity is dependent on calcium ions and diacylglycerol emphasizing its role in calcium and lipid signaling pathways.
PKC alpha known as Protein Kinase C alpha is a serine/threonine-specific protein kinase involved in various cellular processes. The molecular mass of PKC alpha is approximately 80 kDa. Alternately called PKCA or PRKC alpha it is expressed in many tissues with high expression in the brain heart and lungs. PKC alpha plays a role in signal transduction by phosphorylating serine and threonine residues on target proteins.
PKC alpha plays a vital role in the MAPK/ERK pathway and the PI3K/AKT pathway which are critical for the cellular stress response and growth regulation. PKC alpha interacts with several proteins in these pathways including RAF1 and AKT1 facilitating signal transmission that influences cell fate decisions.
PKC alpha has been implicated in cancer and cardiac hypertrophy. In cancers aberrant PKC alpha signaling is linked to increased cell proliferation and survival. In cardiac hypertrophy it is associated with abnormal heart growth due to impaired signaling involving AKT1 and GSK3B. These connections highlight PKC alpha's influence in pathological as well as physiological conditions.
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Terms & Conditions.
Blocking and dilution buffer: 5% NFDM/TBST.
Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag.
Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag.
Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag.
Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag.
Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag.
Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag.
Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag.
Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.
All lanes: Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837) at 1/20000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (AB55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (AB60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (AB60841)
Lane 4: Western blot - Recombinant human PKC delta protein (AB60844)
Lane 5: Western blot - Recombinant human PKC eta protein (AB60849)
Lane 6: Western blot - Recombinant human PKC epsilon protein (AB60847)
Lane 7: Western blot - Recombinant human PKC theta/PRKCQ protein (AB56641)
Lane 8: Western blot - Recombinant human PKC mu/PKD protein (AB60873)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 100-150 kDa
Exposure time: 1s
Blocking and dilution buffer: 5% NFDM/TBST.
Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag.
Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag.
Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag.
Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag.
Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag.
Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag.
Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag.
Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.
Specific activity: 3,200 nmol/min/mg
Purity >95% by densiometry.
5% NFDM/TBST
All positive controls 10 ng
All lanes: Western blot - Anti-PKC alpha antibody [133] (AB11723) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (AB55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (AB60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (AB60841)
Lane 4: Western blot - Recombinant human PKC eta protein (AB60849)
Lane 5: Western blot - Recombinant human PKC epsilon protein (AB60847)
Lane 6: Western blot - Recombinant human PKC theta/PRKCQ protein (AB56641)
Lane 7: Western blot - Recombinant human PKC mu/PKD protein (AB60873)
Lane 8: Western blot - Recombinant human PKC zeta protein (AB60848)
Lane 9: Western blot - Recombinant human PKC iota protein (AB60850)
Lane 10: Western blot - Recombinant human PKC gamma protein (AB60842)
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDa
5% NFDM/TBST
All lanes: Western blot - Anti-PKC alpha antibody [133] (AB11723) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (AB55672)
Lane 2: Western blot - Recombinant human PKC delta protein (AB60844)
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDa
Exposure time: 180s
All lanes: Anti-PKC alpha antibody (ab66181) at 1 µg/mL
All lanes: Western blot - Recombinant human PKC alpha protein (Active) (AB55672) at 0.01 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10s
This data was developed using ab53295, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC delta (phospho T507) antibody [EPR1485Y] (AB53295) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (AB55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (AB60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (AB60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (AB60842)
Lane 5: Western blot - Recombinant human PKC delta protein (AB60844)
Lane 6: Western blot - Recombinant human PKC zeta protein (AB60848)
Lane 7: Western blot - Recombinant human PKC eta protein (AB60849)
Lane 8: Western blot - Recombinant human PKC theta/PRKCQ protein (AB56641)
Lane 9: Western blot - Recombinant human PKC iota protein (AB60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 105 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32376).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC alpha antibody [Y124] (AB32376) at 1/2000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (AB55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (AB60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (AB60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (AB60842)
Lane 5: Western blot - Recombinant human PKC delta protein (AB60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (AB60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (AB60848)
Lane 8: Western blot - Recombinant human PKC eta protein (AB60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (AB56641)
Lane 10: Western blot - Recombinant human PKC iota protein (AB60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 105 kDa
Exposure time: 20s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab235384 was used to detect GST tag.
All lanes: Western blot - Anti-PKC beta 2 antibody [Y125] (AB32026) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC beta 2 protein (AB60841) at 10 ng
Lane 2: Western blot - Recombinant human PKC beta 1 protein (AB60840) at 10 ng
Lane 3: Western blot - Recombinant human PKC delta protein (AB60844) at 10 ng
Lane 4: Western blot - Recombinant human PKC eta protein (AB60849) at 10 ng
Lane 5: Western blot - Recombinant human PKC epsilon protein (AB60847) at 10 ng
Lane 6: Western blot - Recombinant human PKC theta/PRKCQ protein (AB56641) at 10 ng
Lane 7: Western blot - Recombinant human PKC mu/PKD protein (AB60873) at 10 ng
Lane 8: Western blot - Recombinant human PKC alpha protein (Active) (AB55672) at 10 ng
Lane 9: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 10 ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 100 kDa
Blocking and dilution buffer: 5% NFDM/TBST.
ab109539 could detect PKC gamma (pT514), PKC alpha (pT497), PKC beta (pT500), PKC delta (pT507), PKC eta (pT513), PKC epsilon (pT566) and PKC theta (pT538).
Active human PKC alpha full length protein (Catalog#ab55672) contains aa1-672 with GST-tag;
Active human PKC beta 1 full length protein (Catalog#ab60840) contains aa1-671 with GST-tag;
Active human PKC beta 2 full length protein (Catalog#ab60841) contains aa1-673 with GST-tag;
Active human PKC delta full length protein (Catalog#ab60844) contains aa1-676 with GST-tag;
Active human PKC eta full length protein (Catalog#ab60849) contains aa1-683 with GST-tag;
Active human PKC epsilon full length protein (Catalog#ab60847) contains aa1-737 with GST-tag;
Active human PKC theta full length protein (Catalog#ab56641) contains aa1-706 with GST-tag;
Active human PKC mu full length protein (Catalog#ab60873) contains aa1-912 with GST-tag;
Active human PKC gamma full length protein (Catalog#ab60842) contains aa1-697 with GST-tag.
All lanes: Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (AB55672) at 0.5 µg
Lane 2: Western blot - Recombinant human PKC beta 1 protein (AB60840) at 0.2 µg
Lane 3: Active human PKC beta 2 full length protein at 0.2 µg
Lane 4: Western blot - Recombinant human PKC delta protein (AB60844) at 0.05 µg
Lane 5: Western blot - Recombinant human PKC eta protein (AB60849) at 0.5 µg
Lane 6: Western blot - Recombinant human PKC epsilon protein (AB60847) at 1 µg
Lane 7: Western blot - Recombinant human PKC theta/PRKCQ protein (AB56641) at 0.2 µg
Lane 8: Western blot - Recombinant human PKC mu/PKD protein (AB60873) at 1 µg
Lane 9: Western blot - Recombinant human PKC gamma protein (AB60842) at 0.1 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Predicted band size: 77 kDa
Observed band size: 80 kDa
Exposure time: 180s
This data was developed using ab76129, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1-4: 180 seconds; Lane 5-6: 60 seconds; Lane 7-10: 5 seconds.
All lanes: Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (AB76129) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (AB55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (AB60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (AB60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (AB60842)
Lane 5: Western blot - Recombinant human PKC delta protein (AB60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (AB60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (AB60848)
Lane 8: Western blot - Recombinant human PKC eta protein (AB60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (AB56641)
Lane 10: Western blot - Recombinant human PKC iota protein (AB60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Observed band size: 105 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1-4: 180 seconds; Lane 5-6: 60 seconds; Lane 7-10: 5 seconds.
This data was developed using ab180848, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (AB180848) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (AB55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (AB60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (AB60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (AB60842)
Lane 5: Western blot - Recombinant human PKC delta protein (AB60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (AB60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (AB60848)
Lane 8: Western blot - Recombinant human PKC eta protein (AB60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (AB56641)
Lane 10: Western blot - Recombinant human PKC iota protein (AB60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 105 kDa
Exposure time: 60s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62372).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (AB55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (AB60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (AB60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (AB60842)
Lane 5: Western blot - Recombinant human PKC delta protein (AB60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (AB60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (AB60848)
Lane 8: Western blot - Recombinant human PKC eta protein (AB60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (AB56641)
Lane 10: Western blot - Recombinant human PKC iota protein (AB60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 105 kDa
Exposure time: 5s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using ab32502, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC alpha (phospho T638) antibody [E195] (AB32502) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (AB55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (AB60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (AB60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (AB60842)
Lane 5: Western blot - Recombinant human PKC delta protein (AB60844)
Lane 6: Western blot - Recombinant human PKC zeta protein (AB60848)
Lane 7: Western blot - Recombinant human PKC eta protein (AB60849)
Lane 8: Western blot - Recombinant human PKC theta/PRKCQ protein (AB56641)
Lane 9: Western blot - Recombinant human PKC iota protein (AB60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 105 kDa
Exposure time: 60s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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