Skip to main content

Recombinant human PKC beta 2 protein is a Human Full Length protein, in the 1 to 671 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.

Be the first to review this product! Submit a review

Images

Western blot - Recombinant human PKC beta 2 protein (AB60841), expandable thumbnail
  • Functional Studies - Recombinant human PKC beta 2 protein (AB60841), expandable thumbnail
  • Western blot - Recombinant human PKC beta 2 protein (AB60841), expandable thumbnail
  • SDS-PAGE - Recombinant human PKC beta 2 protein (AB60841), expandable thumbnail
  • Functional Studies - Recombinant human PKC beta 2 protein (AB60841), expandable thumbnail

Publications

Key facts

Purity

>70%

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

M A D P A A G P P P S E G E E S T V R F A R K G A L R Q K N V H E V K N H K F T A R F F K Q P T F C S H C T D F I W G F G K Q G F Q C Q V C C F V V H K R C H E F V T F S C P G A D K G P A S D D P R S K H K F K I H T Y S S P T F C D H C G S L L Y G L I H Q G M K C D T C M M N V H K R C V M N V P S L C G T D H T E R R G R I Y I Q A H I D R D V L I V L V R D A K N L V P M D P N G L S D P Y V K L K L I P D P K S E S K Q K T K T I K C S L N P E W N E T F R F Q L K E S D K D R R L S V E I W D W D L T S R N D F M G S L S F G I S E L Q K A S V D G W F K L L S Q E E G E Y F N V P V P P E G S E A N E E L R Q K F E R A K I S Q G T K V P E E K T T N T V S K F D N N G N R D R M K L T D F N F L M V L G K G S F G K V M L S E R K G T D E L Y A V K I L K K D V V I Q D D D V E C T M V E K R V L A L P G K P P F L T Q L H S C F Q T M D R L Y F V M E Y V N G G D L M Y H I Q Q V G R F K E P H A V F Y A A E I A I G L F F L Q S K G I I Y R D L K L D N V M L D S E G H I K I A D F G M C K E N I W D G V T T K T F C G T P D Y I A P E I I A Y Q P Y G K S V D W W A F G V L L Y E M L A G Q A P F E G E D E D E L F Q S I M E H N V A Y P K S M S K E A V A I C K G L M T K H P G K R L G C G P E G E R D I K E H A F F R Y I D W E K L E R K E I Q P P Y K P K A C G R N A E N F D R F F T R H P P V L T P P D Q E V I R N I D Q S E F E G F S F V N S E F L K P E V K S

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity (PubMed:11598012). Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A (PubMed:20228790). In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1 (PubMed:25982116). Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription (PubMed:19176525). Phosphorylates KLHL3 in response to angiotensin II signaling, decreasing the interaction between KLHL3 and WNK4 (PubMed:25313067). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).

Alternative names

PKCB, PRKCB1, PRKCB1, PRKCB, PKCB, Protein kinase C beta type, PKC-B, PKC-beta

Recommended products

Recombinant human PKC beta 2 protein is a Human Full Length protein, in the 1 to 671 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>70%

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, FuncS

Biological activity

423 nmol/min/mg Specific activity defined by radiometric assay, nmol of gamma-phosphate from ATP transferred to PKCtide peptide substrate

Accession
P05771-2
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

Sequence info

Amino acid sequence

M A D P A A G P P P S E G E E S T V R F A R K G A L R Q K N V H E V K N H K F T A R F F K Q P T F C S H C T D F I W G F G K Q G F Q C Q V C C F V V H K R C H E F V T F S C P G A D K G P A S D D P R S K H K F K I H T Y S S P T F C D H C G S L L Y G L I H Q G M K C D T C M M N V H K R C V M N V P S L C G T D H T E R R G R I Y I Q A H I D R D V L I V L V R D A K N L V P M D P N G L S D P Y V K L K L I P D P K S E S K Q K T K T I K C S L N P E W N E T F R F Q L K E S D K D R R L S V E I W D W D L T S R N D F M G S L S F G I S E L Q K A S V D G W F K L L S Q E E G E Y F N V P V P P E G S E A N E E L R Q K F E R A K I S Q G T K V P E E K T T N T V S K F D N N G N R D R M K L T D F N F L M V L G K G S F G K V M L S E R K G T D E L Y A V K I L K K D V V I Q D D D V E C T M V E K R V L A L P G K P P F L T Q L H S C F Q T M D R L Y F V M E Y V N G G D L M Y H I Q Q V G R F K E P H A V F Y A A E I A I G L F F L Q S K G I I Y R D L K L D N V M L D S E G H I K I A D F G M C K E N I W D G V T T K T F C G T P D Y I A P E I I A Y Q P Y G K S V D W W A F G V L L Y E M L A G Q A P F E G E D E D E L F Q S I M E H N V A Y P K S M S K E A V A I C K G L M T K H P G K R L G C G P E G E R D I K E H A F F R Y I D W E K L E R K E I Q P P Y K P K A C G R N A E N F D R F F T R H P P V L T P P D Q E V I R N I D Q S E F E G F S F V N S E F L K P E V K S

Accession

P05771

Protein length

Full Length

Predicted molecular weight

105 kDa

Amino acids

1 to 671

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

Additional notes

Purified by affinity chromatography

General info

Function

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity (PubMed:11598012). Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A (PubMed:20228790). In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1 (PubMed:25982116). Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription (PubMed:19176525). Phosphorylates KLHL3 in response to angiotensin II signaling, decreasing the interaction between KLHL3 and WNK4 (PubMed:25313067). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Post-translational modifications

Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade (By similarity).

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

CREB peptide ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Protein kinase C beta 2 also known as PKC beta 2 or PRKCB is an important isoform of the PKC family with a molecular weight of 79 kDa. PKC proteins are a group of serine/threonine kinases involved in various cellular processes. PKC beta 2 expresses in tissues like the spleen lymph nodes and other immune-related tissues. The protein plays a significant role in immune responses and its expression is regulated by various signaling molecules.

Biological function summary

PKC beta 2 acts as an important regulator in the immune system by modulating immune cell activation and proliferation. It functions as part of the PKC protein complex which interacts with lipids and calcium as cofactors. This protein has critical influence on processes such as B-cell activation and phagocyte response. It also regulates several transcription factors impacting gene expression critical for the immune system.

Pathways

PKC beta 2 integrates into multiple signaling pathways including the NF-kB and MAPK pathways. These pathways are essential for transmitting signals from the cell surface to the nucleus affecting gene expression and cell function. PKC beta 2 directly influences protein interactions within these pathways such as with other PKC family members and other proteins like IKK which is critical in the NF-kB signaling pathway.

Associated diseases and disorders

PKC beta 2 is significantly connected to certain cancers and autoimmune conditions. Aberrant PKC beta 2 activity is linked to chronic lymphocytic leukemia where it affects the survival and proliferation of B cells. It is also involved in diabetic nephropathy contributing to kidney damage through excessive signaling. In these conditions PKC beta 2 interacts with proteins like Bcl-2 in cancer and TGF-beta in diabetic nephropathy influencing the disease courses and potentially serving as targets for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com