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Recombinant human PKC eta protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.

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Images

Functional Studies - Recombinant human PKC eta protein (AB60849), expandable thumbnail
  • Western blot - Recombinant human PKC eta protein (AB60849), expandable thumbnail
  • Western blot - Recombinant human PKC eta protein (AB60849), expandable thumbnail
  • SDS-PAGE - Recombinant human PKC eta protein (AB60849), expandable thumbnail
  • Functional Studies - Recombinant human PKC eta protein (AB60849), expandable thumbnail

Publications

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.

Alternative names

PKCL, PRKCL, PRKCL, PKCL, PRKCH, Protein kinase C eta type, PKC-L, nPKC-eta

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Recombinant human PKC eta protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, FuncS

Biological activity

Active

Accession
P24723-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

Sequence info

Amino acid sequence

Accession

P24723

Protein length

Full Length

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

PKC epsilon peptide ab204875 (PKC epsilon peptide) can be utilized as a substrate for assessing kinase activity

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Protein kinase C eta (PKC eta) is an enzyme also known as PKC η which belongs to the PKC family of serine/threonine kinases. This protein has a molecular mass of approximately 80 kDa and shows expression in numerous tissues with strong presence in epithelia and the immune system. PKC eta's mechanical function involves phosphorylation of serine and threonine residues on cellular target proteins modulating their activity. It plays a significant role in signal transduction pathways that govern various cellular processes.

Biological function summary

PKC eta involves itself in cellular differentiation and proliferation. It is a component of signal transduction complexes that mediate these processes. In epithelial cells PKC eta modulates cell adhesion and integrity. It also affects immune responses through its action in T-cells and other immune components impacting both innate and adaptive immunity. The enzyme interacts closely with other PKC isoforms allowing it to regulate diverse biological functions through complex networks.

Pathways

PKC eta plays an integral role in growth factor and cytokine signaling pathways. MAPK/ERK and PI3K/AKT pathways include PKC eta as key components for cell survival and proliferation. These pathways facilitate interactions and regulation involving proteins like RAF-1 and AKT coordinating cell growth and apoptosis. PKC eta's positioning in these pathways denotes its significance in maintaining cellular homeostasis and responding to external stimuli.

Associated diseases and disorders

PKC eta implicates itself in autoimmune conditions like rheumatoid arthritis and in certain types of cancer such as breast cancer. PKC eta influences inflammatory responses often exacerbating autoimmune phenomena by interacting with cytokines and immune receptors. In cancer it may contribute to tumorigenesis by promoting cell survival through PI3K/AKT pathway engagement. It connects with proteins like NF-kB driving pathological changes in cell behavior and immune modulation.

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