Recombinant human PKC zeta protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
>90% SDS-PAGE
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, WB, FuncS
Yes
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab60848 can be used as a WB positive control in conjunction with ab51157. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes Specific Activity: 114 nmol/min/mg. |
Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed:17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).Isoform 2Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.
PKC2, PRKCZ, PKC2, Protein kinase C zeta type, nPKC-zeta
Recombinant human PKC zeta protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
>90% SDS-PAGE
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, WB, FuncS
Yes
Active
No
Human
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Full Length
Recombinant
GST tag N-Terminus
Liquid
Purity: >90% as determined by densitometry. Affinity purified.
Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed:17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase.
Endosome
Dry Ice
-80°C
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
CREB peptide ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
This supplementary information is collated from multiple sources and compiled automatically.
PKC zeta also known as Protein Kinase C zeta is a member of the atypical protein kinase C family. This enzyme plays an important role in various cellular processes. PKC zeta has a molecular mass of about 55 kDa and is expressed in many tissues including brain and immune cells. Unlike conventional PKC enzymes PKC zeta does not respond to calcium or diacylglycerol which influences its unique regulatory roles.
PKC zeta participates in regulating cell proliferation survival and differentiation. It often functions as part of signaling complexes that include other proteins like PDK1 and is involved in transducing signals from cell surface receptors to the nucleus. Its activity influences cellular responses to hormones growth factors and cytokines contributing to diverse cellular events.
PKC zeta plays a critical role in the NF-kappaB and PI3K/Akt signaling pathways. In the NF-kappaB pathway PKC zeta helps in the activation of this transcription factor affecting genes related to inflammation and immune response. In the PI3K/Akt pathway it is involved in cell growth and survival interacting with kinases such as Akt and PDK1 which further modulate downstream effectors.
PKC zeta has associations with cancer and insulin resistance-related conditions. In cancer dysregulation of PKC zeta can lead to unchecked cellular proliferation due to its involvement in survival pathways and it often interacts with oncogenic proteins like Akt. In insulin resistance PKC zeta misregulation impacts glucose metabolism highlighting its role in diabetes-related pathways alongside insulin receptor substrates.
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Terms & Conditions.
The specific activity of PKC zeta (ab60848) was determined to be 103 nmol/min/mg as per activity assay protocol
SDS PAGE analysis of ab60848
5% NFDM/TBST
All positive controls 10 ng
All lanes: Western blot - Anti-PKC alpha antibody [133] (Anti-PKC alpha antibody [133] ab11723) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 5: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)
Lane 6: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 7: Western blot - Recombinant human PKC mu/PKD protein (Recombinant human PKC mu/PKD protein ab60873)
Lane 8: Western blot - Recombinant human PKC zeta protein (ab60848)
Lane 9: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
Lane 10: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDa
Sample Kinase Activity Plot.
ab60848 on SDS-PAGE, MW ~93 kDa.
All lanes: Anti-PKC zeta antibody (ab51157) at 1/1000 dilution
All lanes: Western blot - Recombinant human PKC zeta protein (ab60848) at 0.01 µg
All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1min
This data was developed using Anti-PKC delta (phospho T507) antibody [EPR1485Y] ab53295, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC delta (phospho T507) antibody [EPR1485Y] (Anti-PKC delta (phospho T507) antibody [EPR1485Y] ab53295) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)
Lane 6: Western blot - Recombinant human PKC zeta protein (ab60848)
Lane 7: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 8: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 9: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 105 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] ab76129, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1-4: 180 seconds; Lane 5-6: 60 seconds; Lane 7-10: 5 seconds.
All lanes: Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] ab76129) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (ab60848)
Lane 8: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 10: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 105 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1-4: 180 seconds; Lane 5-6: 60 seconds; Lane 7-10: 5 seconds.
This data was developed using Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] ab180848, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] ab180848) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (ab60848)
Lane 8: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 10: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 105 kDa
Exposure time: 60s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PKC zeta (phospho T560) antibody [EP2037AY] ab62372).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (Anti-PKC zeta (phospho T560) antibody [EP2037AY] ab62372) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (ab60848)
Lane 8: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 10: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 105 kDa
Exposure time: 5s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using Anti-PKC alpha (phospho T638) antibody [E195] ab32502, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC alpha (phospho T638) antibody [E195] (Anti-PKC alpha (phospho T638) antibody [E195] ab32502) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)
Lane 6: Western blot - Recombinant human PKC zeta protein (ab60848)
Lane 7: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 8: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 9: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 105 kDa
Exposure time: 60s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PKC alpha antibody [Y124] ab32376).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC alpha antibody [Y124] (Anti-PKC alpha antibody [Y124] ab32376) at 1/2000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (ab60848)
Lane 8: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 10: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 105 kDa
Exposure time: 20s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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