Recombinant human PKLR protein is a Human Full Length protein, in the 2 to 574 aa range, expressed in Escherichia coli, with >=80% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
S I Q E N I S S L Q L R S W V S K S Q R D L A K S I L I G A P G G P A G Y L R R A S V A Q L T Q E L G T A F F Q Q Q Q L P A A M A D T F L E H L C L L D I D S E P V A A R S T S I I A T I G P A S R S V E R L K E M I K A G M N I A R L N F S H G S H E Y H A E S I A N V R E A V E S F A G S P L S Y R P V A I A L D T K G P E I R T G I L Q G G P E S E V E L V K G S Q V L V T V D P A F R T R G N A N T V W V D Y P N I V R V V P V G G R I Y I D D G L I S L V V Q K I G P E G L V T Q V E N G G V L G S R K G V N L P G A Q V D L P G L S E Q D V R D L R F G V E H G V D I V F A S F V R K A S D V A A V R A A L G P E G H G I K I I S K I E N H E G V K R F D E I L E V S D G I M V A R G D L G I E I P A E K V F L A Q K M M I G R C N L A G K P V V C A T Q M L E S M I T K P R P T R A E T S D V A N A V L D G A D C I M L S G E T A K G N F P V E A V K M Q H A I A R E A E A A V Y H R Q L F E E L R R A A P L S R D P T E V T A I G A V E A A F K C C A A A I I V L T T T G R S A Q L L S R Y R P R A A V I A V T R S A Q A A R Q V H L C R G V F P L L Y R E P P E A I W A D D V D R R V Q F G I E S G K L R G F L R V G D L V I V V T G W R P G S G Y T N I M R V L S I S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.
Alternative names
PK1, PKL, PKLR, Pyruvate kinase PKLR, Pyruvate kinase 1, Pyruvate kinase isozymes L/R, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase
Recommended products
Recombinant human PKLR protein is a Human Full Length protein, in the 2 to 574 aa range, expressed in Escherichia coli, with >=80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: ≥36 pmol/min/μg.
A 50 μl reaction is conducted in a buffer containing 50 mM Tris (pH 7.4), 10 mM MgCl2, 50 mM KCl, 2 mM ADP, 10 mM phosphoenolpyruvate (PEP) at room temperature for 15 min. ATP production is detected.
- Accession
- P30613-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.48% Tris, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- S I Q E N I S S L Q L R S W V S K S Q R D L A K S I L I G A P G G P A G Y L R R A S V A Q L T Q E L G T A F F Q Q Q Q L P A A M A D T F L E H L C L L D I D S E P V A A R S T S I I A T I G P A S R S V E R L K E M I K A G M N I A R L N F S H G S H E Y H A E S I A N V R E A V E S F A G S P L S Y R P V A I A L D T K G P E I R T G I L Q G G P E S E V E L V K G S Q V L V T V D P A F R T R G N A N T V W V D Y P N I V R V V P V G G R I Y I D D G L I S L V V Q K I G P E G L V T Q V E N G G V L G S R K G V N L P G A Q V D L P G L S E Q D V R D L R F G V E H G V D I V F A S F V R K A S D V A A V R A A L G P E G H G I K I I S K I E N H E G V K R F D E I L E V S D G I M V A R G D L G I E I P A E K V F L A Q K M M I G R C N L A G K P V V C A T Q M L E S M I T K P R P T R A E T S D V A N A V L D G A D C I M L S G E T A K G N F P V E A V K M Q H A I A R E A E A A V Y H R Q L F E E L R R A A P L S R D P T E V T A I G A V E A A F K C C A A A I I V L T T T G R S A Q L L S R Y R P R A A V I A V T R S A Q A A R Q V H L C R G V F P L L Y R E P P E A I W A D D V D R R V Q F G I E S G K L R G F L R V G D L V I V V T G W R P G S G Y T N I M R V L S I S
- Accession
- P30613
- Protein length
- Full Length
- Predicted molecular weight
- 63 kDa
- Amino acids
- 2 to 574
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.
- Sequence similarities
Belongs to the pyruvate kinase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PKLR gene encodes the pyruvate kinase liver and red blood cell enzyme also known as PK-LR. This enzyme catalyzes the last step in the glycolytic pathway converting phosphoenolpyruvate (PEP) into pyruvate with the production of ATP. The alternative names for PKLR include R-type pyruvate kinase and L-type pyruvate kinase. The enzyme has a molecular mass of approximately 57 kDa. It is primarily expressed in the liver and red blood cells with some presence in the pancreatic islets supporting its physiological roles in these tissues.
- Biological function summary
Pyruvate kinase is important for energy production by regulating glycolysis. It exists as a homo-tetramer forming a complex to execute its enzymatic activity. This complex ensures the efficient conversion of PEP facilitating the flow of carbon through the glycolytic pathway. In the liver PKLR plays a role in gluconeogenesis and lipogenesis while in red blood cells it contributes directly to ATP production essential for maintaining cellular energy and function.
- Pathways
The pyruvate kinase enzyme is a significant participant in glycolysis and gluconeogenesis. These pathways are central to energy metabolism linking glucose breakdown with ATP generation. Pyruvate kinase interacts with enzymes like hexokinase and phosphofructokinase within the glycolytic pathway coordinating with these enzymes to regulate energy metabolism. In gluconeogenesis PKLR integrates into the mechanism ensuring the balance between energy production and storage.
- Associated diseases and disorders
PKLR mutations can lead to pyruvate kinase deficiency a condition that causes hemolytic anemia. This disorder results from impaired ATP production in red blood cells causing them to break down prematurely. In liver tissues dysregulation of PKLR activity associates with cancers like hepatocellular carcinoma. This enzyme connects with proteins such as lactate dehydrogenase in cancer metabolism affecting the Warburg effect which is tangled with cancer cell survival and proliferation.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Recombinant human PKLR protein (ab196100)
Kinase assay using ab196100.
SDS-PAGE - Recombinant human PKLR protein (ab196100)
10% SDS-PAGE analysis of 7 μg ab196100 with Coomassie staining.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com