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AB196100

Recombinant human PKLR protein (His tag N-Terminus)

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Recombinant human PKLR protein (His tag N-Terminus) is a Human Full Length protein, in the 2 to 574 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PK1, PKL, PKLR, Pyruvate kinase PKLR, Pyruvate kinase 1, Pyruvate kinase isozymes L/R, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

2 Images
Functional Studies - Recombinant human PKLR protein (His tag N-Terminus) (AB196100)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PKLR protein (His tag N-Terminus) (AB196100)

Kinase assay using ab196100.

SDS-PAGE - Recombinant human PKLR protein (His tag N-Terminus) (AB196100)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PKLR protein (His tag N-Terminus) (AB196100)

10% SDS-PAGE analysis of 7 μg ab196100 with Coomassie staining.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥36 pmol/min/μg.

A 50 μl reaction is conducted in a buffer containing 50 mM Tris (pH 7.4), 10 mM MgCl2, 50 mM KCl, 2 mM ADP, 10 mM phosphoenolpyruvate (PEP) at room temperature for 15 min. ATP production is detected.

Accession

P30613

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.48% Tris, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

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Complementary Products

Assay Kits

AB83432

Pyruvate Kinase Assay Kit

Functional Studies - Pyruvate Kinase Assay Kit (AB83432)

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  • 0
Primary Antibodies

AB171744

Anti-PKLR antibody [EPR11093]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (AB171744)

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  • 1
Proteins & Peptides

AB152216

Recombinant Human ATP7b protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ATP7b protein (AB152216)

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  • 0
Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS","proteinLength":"Full Length","predictedMolecularWeight":"63 kDa","actualMolecularWeight":null,"aminoAcidEnd":574,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30613","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PKLR gene encodes the pyruvate kinase liver and red blood cell enzyme also known as PK-LR. This enzyme catalyzes the last step in the glycolytic pathway converting phosphoenolpyruvate (PEP) into pyruvate with the production of ATP. The alternative names for PKLR include R-type pyruvate kinase and L-type pyruvate kinase. The enzyme has a molecular mass of approximately 57 kDa. It is primarily expressed in the liver and red blood cells with some presence in the pancreatic islets supporting its physiological roles in these tissues.
Biological function summary

Pyruvate kinase is important for energy production by regulating glycolysis. It exists as a homo-tetramer forming a complex to execute its enzymatic activity. This complex ensures the efficient conversion of PEP facilitating the flow of carbon through the glycolytic pathway. In the liver PKLR plays a role in gluconeogenesis and lipogenesis while in red blood cells it contributes directly to ATP production essential for maintaining cellular energy and function.

Pathways

The pyruvate kinase enzyme is a significant participant in glycolysis and gluconeogenesis. These pathways are central to energy metabolism linking glucose breakdown with ATP generation. Pyruvate kinase interacts with enzymes like hexokinase and phosphofructokinase within the glycolytic pathway coordinating with these enzymes to regulate energy metabolism. In gluconeogenesis PKLR integrates into the mechanism ensuring the balance between energy production and storage.

PKLR mutations can lead to pyruvate kinase deficiency a condition that causes hemolytic anemia. This disorder results from impaired ATP production in red blood cells causing them to break down prematurely. In liver tissues dysregulation of PKLR activity associates with cancers like hepatocellular carcinoma. This enzyme connects with proteins such as lactate dehydrogenase in cancer metabolism affecting the Warburg effect which is tangled with cancer cell survival and proliferation.
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Specifications

Form

Liquid

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General info

Function

Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.

Sequence similarities

Belongs to the pyruvate kinase family.

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Product protocols

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Target data

Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.
See full target information PKLR
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