Recombinant Human PLA2G7 protein is a Human Full Length protein, in the 22 to 441 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, ELISA.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, ELISA
- Biologically active
- No
Amino acid sequence
F D W Q Y I N P V A H M K S S A W V N K I Q V L M A A A S F G Q T K I P R G N G P Y S V G C T D L M F D H T N K G T F L R L Y Y P S Q D N D R L D T L W I P N K E Y F W G L S K F L G T H W L M G N I L R L L F G S M T T P A N W N S P L R P G E K Y P L V V F S H G L G A F R T L Y S A I G I D L A S H G F I V A A V E H R D R S A S A T Y Y F K D Q S A A E I G D K S W L Y L R T L K Q E E E T H I R N E Q V R Q R A K E C S Q A L S L I L D I D H G K P V K N A L D L K F D M E Q L K D S I D R E K I A V I G H S F G G A T V I Q T L S E D Q R F R C G I A L D A W M F P L G D E V Y S R I P Q P L F F I N S E Y F Q Y P A N I I K M K K C Y S P D K E R K M I T I R G S V H Q N F A D F T F A T G K I I G H M L K L K G D I D S N V A I D L S N K A S L A F L Q K H L G L H K D F D Q W D C L I E G D D E N L I P G T N I N T T N Q H I M L Q N S S G I E K Y N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response (PubMed:10066756, PubMed:16371369, PubMed:17090529, PubMed:2040620, PubMed:7700381, PubMed:8624782). At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed:10504265, PubMed:2040620). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position (PubMed:2040620). Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups (PubMed:2040620, PubMed:8624782). Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells (PubMed:10066756, PubMed:10504265, PubMed:11590221, PubMed:16371369, PubMed:18434304, PubMed:7592717, PubMed:7700381, PubMed:8624782, PubMed:8675689). Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects (PubMed:2040620, PubMed:7700381). As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall (PubMed:17090529). Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage (PubMed:16371369).
Alternative names
PAFAH, PLA2G7, Platelet-activating factor acetylhydrolase, PAF acetylhydrolase, 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, Group-VIIA phospholipase A2, LDL-associated phospholipase A2, PAF 2-acylhydrolase, gVIIA-PLA2, LDL-PLA(2)
Recommended products
Recombinant Human PLA2G7 protein is a Human Full Length protein, in the 22 to 441 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, ELISA.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, ELISA
- Biologically active
- No
- Accession
- Q13093-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.03% EDTA
Sequence info
Amino acid sequence
- F D W Q Y I N P V A H M K S S A W V N K I Q V L M A A A S F G Q T K I P R G N G P Y S V G C T D L M F D H T N K G T F L R L Y Y P S Q D N D R L D T L W I P N K E Y F W G L S K F L G T H W L M G N I L R L L F G S M T T P A N W N S P L R P G E K Y P L V V F S H G L G A F R T L Y S A I G I D L A S H G F I V A A V E H R D R S A S A T Y Y F K D Q S A A E I G D K S W L Y L R T L K Q E E E T H I R N E Q V R Q R A K E C S Q A L S L I L D I D H G K P V K N A L D L K F D M E Q L K D S I D R E K I A V I G H S F G G A T V I Q T L S E D Q R F R C G I A L D A W M F P L G D E V Y S R I P Q P L F F I N S E Y F Q Y P A N I I K M K K C Y S P D K E R K M I T I R G S V H Q N F A D F T F A T G K I I G H M L K L K G D I D S N V A I D L S N K A S L A F L Q K H L G L H K D F D Q W D C L I E G D D E N L I P G T N I N T T N Q H I M L Q N S S G I E K Y N
- Accession
- Q13093
- Protein length
- Full Length
- Predicted molecular weight
- 50 kDa
- Amino acids
- 22 to 441
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response (PubMed:10066756, PubMed:16371369, PubMed:17090529, PubMed:2040620, PubMed:7700381, PubMed:8624782). At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed:10504265, PubMed:2040620). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position (PubMed:2040620). Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups (PubMed:2040620, PubMed:8624782). Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells (PubMed:10066756, PubMed:10504265, PubMed:11590221, PubMed:16371369, PubMed:18434304, PubMed:7592717, PubMed:7700381, PubMed:8624782, PubMed:8675689). Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects (PubMed:2040620, PubMed:7700381). As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall (PubMed:17090529). Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage (PubMed:16371369).
- Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family.
- Post-translational modifications
N-glycosylated. Macrophage-derived PLA2G7 carries sialylated complex-type N-glycans that hinder its binding to HDL particles.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
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SDS-PAGE - Recombinant Human PLA2G7 protein (ab166875)
SDS-PAGE analysis of ab166875.
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