JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB127241

Recombinant Human Plakophilin 1 protein (His-DHFR tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human Plakophilin 1 protein (His-DHFR tag N-Terminus) is a Human Fragment protein, in the 54 to 317 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

Plakophilin-1, Band 6 protein, B6P, PKP1

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q13835

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB183512

Anti-Plakophilin 1 antibody [EPR14890]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Plakophilin 1 antibody [EPR14890] (AB183512)

  • 0
  • 0
Proteins & Peptides

AB70187

Recombinant Human beta Tubulin protein

Western blot - Recombinant Human beta Tubulin protein (AB70187)

  • 0
  • 0
Proteins & Peptides

AB318253

Recombinant Human HSP90 alpha Protein (His-tag)

Mass Spectrometry - Recombinant Human HSP90 alpha Protein (His-tag) (AB318253)

  • 0
  • 0
Primary Antibodies

AB109441

Anti-Plakophilin 3 antibody [EPR5560]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plakophilin 3 antibody [EPR5560] (AB109441)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"29.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":317,"aminoAcidStart":54,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q13835","tags":[{"tag":"His-DHFR","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plakophilin 1 also known as PKP1 is an important component of the cell's structural and signaling machinery. It is a member of the arm-repeat family of proteins and has a molecular mass of around 83 kDa. Plakophilin 1 is expressed in various epithelial tissues where it plays an important role in stabilizing cell junctions. It localizes mainly to desmosomes which are adhesive structures that connect cells together maintaining tissue integrity.
Biological function summary

This protein is a core part of desmosomal plaques which are thickened structures on the inner surface of cell membranes where desmosomes attach. Plakophilin 1 assists in linking the cadherin-catenin complex to intermediate filaments in the cytoskeleton promoting cell-cell adhesion. It is part of a larger protein complex that ensures mechanical strength and resilience across epithelial layers.

Pathways

Plakophilin 1 is involved in the assembly and organization of desmosomal junctions which are part of the broader adherens junction pathway. It interacts with several key proteins including desmoplakin and plakoglobin contributing to the stability and function of cell adhesions. These interactions are essential for maintaining the structural integrity and signaling capabilities of epithelial tissues.

Plakophilin 1 mutations have been linked to ectodermal dysplasia-skin fragility syndrome a condition characterized by compromised skin integrity and hair anomalies. This protein is also associated with certain types of cardiomyopathy due to its role in desmosomal structure and cell adhesion. Its interaction with desmoplakin is particularly relevant in these disorders where faulty cell junctions lead to tissue fragility and pathological conditions.
style
left-column

Specifications

Form

Lyophilized

Additional notes

Purified via His tag

style
left-column

General info

Function

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 23444369). Plays a role in desmosome protein expression regulation and localization to the desmosomal plaque, thereby maintaining cell sheet integrity and anchorage of desmosomes to intermediate filaments (PubMed : 10852826, PubMed : 23444369). Required for localization of DSG3 and YAP1 to the cell membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, YAP1, PKP1 and YWHAG (PubMed : 31835537). Positively regulates differentiation of keratinocytes, potentially via promoting localization of DSG1 at desmosome cell junctions (By similarity). Required for calcium-independent development and maturation of desmosome plaques specifically at lateral cell-cell contacts in differentiating keratinocytes (By similarity). Plays a role in the maintenance of DSG3 protein abundance, DSG3 clustering and localization of these clusters to the cell membrane in keratinocytes (By similarity). May also promote keratinocyte proliferation and morphogenesis during postnatal development (PubMed : 9326952). Required for tight junction inside-out transepidermal barrier function of the skin (By similarity). Promotes Wnt-mediated proliferation and differentiation of ameloblasts, via facilitating TJP1/ZO-1 localization to tight junctions (By similarity). Binds single-stranded DNA (ssDNA), and may thereby play a role in sensing DNA damage and promoting cell survival (PubMed : 20613778). Positively regulates cap-dependent translation and as a result cell proliferation, via recruitment of EIF4A1 to the initiation complex and promotion of EIF4A1 ATPase activity (PubMed : 20156963, PubMed : 23444369). Regulates the mRNA stability and protein abundance of desmosome components PKP2, PKP3, DSC2 and DSP, potentially via its interaction with FXR1 (PubMed : 25225333).

Sequence similarities

Belongs to the beta-catenin family.

Post-translational modifications

Phosphorylated by AKT2; required for interaction with YWHAG and subsequent localization away from desmosomes to the cytoplasm (By similarity). Phosphorylation of Ser-118 by AKT2 promotes PKP1-driven cap-dependent mRNA translation and decreases intercellular adhesion, phosphorylation is promoted by insulin (PubMed:23444369). Phosphorylation by RIPK4 at the N-terminus is required for its role in differentiation of keratinocytes and DSG1 localization at cell junctions (By similarity).

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 23444369). Plays a role in desmosome protein expression regulation and localization to the desmosomal plaque, thereby maintaining cell sheet integrity and anchorage of desmosomes to intermediate filaments (PubMed : 10852826, PubMed : 23444369). Required for localization of DSG3 and YAP1 to the cell membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, YAP1, PKP1 and YWHAG (PubMed : 31835537). Positively regulates differentiation of keratinocytes, potentially via promoting localization of DSG1 at desmosome cell junctions (By similarity). Required for calcium-independent development and maturation of desmosome plaques specifically at lateral cell-cell contacts in differentiating keratinocytes (By similarity). Plays a role in the maintenance of DSG3 protein abundance, DSG3 clustering and localization of these clusters to the cell membrane in keratinocytes (By similarity). May also promote keratinocyte proliferation and morphogenesis during postnatal development (PubMed : 9326952). Required for tight junction inside-out transepidermal barrier function of the skin (By similarity). Promotes Wnt-mediated proliferation and differentiation of ameloblasts, via facilitating TJP1/ZO-1 localization to tight junctions (By similarity). Binds single-stranded DNA (ssDNA), and may thereby play a role in sensing DNA damage and promoting cell survival (PubMed : 20613778). Positively regulates cap-dependent translation and as a result cell proliferation, via recruitment of EIF4A1 to the initiation complex and promotion of EIF4A1 ATPase activity (PubMed : 20156963, PubMed : 23444369). Regulates the mRNA stability and protein abundance of desmosome components PKP2, PKP3, DSC2 and DSP, potentially via its interaction with FXR1 (PubMed : 25225333).
See full target information PKP1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com