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AB174025

Recombinant human PLGF protein (Active)

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(1 Publication)

Recombinant human PLGF protein (Active) is a Human Full Length protein, in the 19 to 170 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

PGFL, PLGF, PGF, Placenta growth factor, PlGF

3 Images
Functional Studies - Recombinant human PLGF protein (Active) (AB174025)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PLGF protein (Active) (AB174025)

Immobilized Rhesus macaque VEGF R1, Mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind ab174025 with a linear range of 5-40 ng/mL

Functional Studies - Recombinant human PLGF protein (Active) (AB174025)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PLGF protein (Active) (AB174025)

Immobilized ab174025 at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1, His,Avitag (ab246160) with a linear range of 2-20 ng/mL

SDS-PAGE - Recombinant human PLGF protein (Active) (AB174025)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PLGF protein (Active) (AB174025)

SDS-PAGE analysis of reduced ab174025 stained overnight with Coomassie Blue.

DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Measured in a cell proliferation assay using MDA - MB – 231 Human breast cancer cells.

The ED50 for this effect is typically 1 - 10 μg/ml.

Accession

P49763

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute with 100mM acetic acid to a concentration of 200 µg/ml.

Storage buffer

pH: 7.4 Constituents: 10% Trehalose, 0.6% Acetic acid

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

The lyophilized product can be stored at 4°C or -80°C for 1 year. After reconstitution, store under sterile conditions for 1 month at 4°C - 8°C or 3 months at -70°C. Avoid repeated freeze-thaw cycles.

Sequence info

[{"sequence":"LPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYVELTFSQHVRCECRPLREKMKPERRRPKGRGKRRREKQRPTDCHLCGDAVPRR","proteinLength":"Full Length","predictedMolecularWeight":"20.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":170,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P49763","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
True

Specifications

Form

Lyophilized

General info

Function

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

Post-translational modifications

N-glycosylated.

Product protocols

Target data

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
See full target information PGF

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Microcirculation (New York, N.Y. : 1994) 21:368-79 PubMed24410720

2014

Placenta growth factor and vascular endothelial growth factor a have differential, cell-type specific patterns of expression in vascular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjin Xiang,Rohan Varshney,Nabil A Rashdan,Jennifer H Shaw,Pamela G Lloyd
View all publications

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