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Recombinant human PLK1 protein is a Human Full Length protein, in the 1 to 603 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for WB, FuncS, SDS-PAGE, IHC-P.

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Key facts

Purity

>70%

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus

Applications

WB, FuncS, SDS-PAGE, IHC-P

Biologically active

Yes

Amino acid sequence

M S A A V T A G K L A R A P A D P G K A G V P G V A A P G A P A A A P P A K E I P E V L V D P R S R R R Y V R G R F L G K G G F A K C F E I S D A D T K E V F A G K I V P K S L L L K P H Q R E K M S M E I S I H R S L A H Q H V V G F H G F F E D N D F V F V V L E L C R R R S L L E L H K R R K A L T E P E A R Y Y L R Q I V L G C Q Y L H R N R V I H R D L K L G N L F L N E D L E V K I G D F G L A T K V E Y D G E R K K T L C G T P N Y I A P E V L S K K G H S F E V D V W S I G C I M Y T L L V G K P P F E T S C L K E T Y L R I K K N E Y S I P K H I N P V A A S L I Q K M L Q T D P T A R P T I N E L L N D E F F T S G Y I P A R L P I T C L T I P P R F S I A P S S L D P S N R K P L T V L N K G L E N P L P E R P R E K E E P V V R E T G E V V D C H L S D M L Q Q L H S V N A S K P S E R G L V R Q E E A E D P A C I P I F W V S K W V D Y S D K Y G L G Y Q L C D N S V G V L F N D S T R L I L Y N D G D S L Q Y I E R D G T E S Y L T V S S H P N S L M K K I T L L K Y F R N Y M S E H L L K A G A N I T P R E G D E L A R L P Y L R T W F R T R S A I I L H L S N G S V Q I N F F Q D H T K L I L C P L M A A V T Y I D E K R D F R T Y R L S L L E E Y G C C K E L A S R L R Y A R T M V D K L L S S R S A S N R L K A S

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Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

Ab51426 can be used as a WB positive control in conjunction with ab47867.

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

Kinase activity assay.

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

Molecular weight 70 kDa.

Application

IHC-P

Reactivity

Reacts

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Target data

Function

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGO1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, WEE1 and HNRNPU. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Phosphorylation of NINL component of the centrosome leads to NINL dissociation from other centrosomal proteins. Involved in mitosis exit and cytokinesis by phosphorylating CEP55, ECT2, KIF20A/MKLP2, CENPU, PRC1 and RACGAP1. Recruited at the central spindle by phosphorylating and docking PRC1 and KIF20A/MKLP2; creates its own docking sites on PRC1 and KIF20A/MKLP2 by mediating phosphorylation of sites subsequently recognized by the POLO box domains. Phosphorylates RACGAP1, thereby creating a docking site for the Rho GTP exchange factor ECT2 that is essential for the cleavage furrow formation. Promotes the central spindle recruitment of ECT2. Plays a central role in G2/M transition of mitotic cell cycle by phosphorylating CCNB1, CDC25C, FOXM1, CENPU, PKMYT1/MYT1, PPP1R12A/MYPT1 and WEE1. Part of a regulatory circuit that promotes the activation of CDK1 by phosphorylating the positive regulator CDC25C and inhibiting the negative regulators WEE1 and PKMYT1/MYT1. Also acts by mediating phosphorylation of cyclin-B1 (CCNB1) on centrosomes in prophase. Phosphorylates FOXM1, a key mitotic transcription regulator, leading to enhance FOXM1 transcriptional activity. Involved in kinetochore functions and sister chromatid cohesion by phosphorylating BUB1B/BUBR1, FBXO5/EMI1 and STAG2/SA2. PLK1 is high on non-attached kinetochores suggesting a role of PLK1 in kinetochore attachment or in spindle assembly checkpoint (SAC) regulation. Required for kinetochore localization of BUB1B. Regulates the dissociation of cohesin from chromosomes by phosphorylating cohesin subunits such as STAG2/SA2. Phosphorylates SGO1: required for spindle pole localization of isoform 3 of SGO1 and plays a role in regulating its centriole cohesion function. Mediates phosphorylation of FBXO5/EMI1, a negative regulator of the APC/C complex during prophase, leading to FBXO5/EMI1 ubiquitination and degradation by the proteasome. Acts as a negative regulator of p53 family members: phosphorylates TOPORS, leading to inhibit the sumoylation of p53/TP53 and simultaneously enhance the ubiquitination and subsequent degradation of p53/TP53. Phosphorylates the transactivation domain of the transcription factor p73/TP73, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Also required for recovery after DNA damage checkpoint and entry into mitosis. Phosphorylates MISP, leading to stabilization of cortical and astral microtubule attachments required for proper spindle positioning (PubMed:8991084, PubMed:11202906, PubMed:12207013, PubMed:12447691, PubMed:12524548, PubMed:12738781, PubMed:12852856, PubMed:12939256, PubMed:14532005, PubMed:14734534, PubMed:15070733, PubMed:15148369, PubMed:15469984, PubMed:16198290, PubMed:16247472, PubMed:16980960, PubMed:17081991, PubMed:17351640, PubMed:17376779, PubMed:17617734, PubMed:18174154, PubMed:18331714, PubMed:18418051, PubMed:18477460, PubMed:18521620, PubMed:18615013, PubMed:19160488, PubMed:19351716, PubMed:19468300, PubMed:19468302, PubMed:19473992, PubMed:19509060, PubMed:19597481, PubMed:23455478, PubMed:23509069). Together with MEIKIN, acts as a regulator of kinetochore function during meiosis I: required both for mono-orientation of kinetochores on sister chromosomes and protection of centromeric cohesin from separase-mediated cleavage (By similarity). Phosphorylates CEP68 and is required for its degradation (PubMed:25503564). Regulates nuclear envelope breakdown during prophase by phosphorylating DCTN1 resulting in its localization in the nuclear envelope (PubMed:20679239). Phosphorylates the heat shock transcription factor HSF1, promoting HSF1 nuclear translocation upon heat shock (PubMed:15661742). Phosphorylates HSF1 also in the early mitotic period; this phosphorylation regulates HSF1 localization to the spindle pole, the recruitment of the SCF(BTRC) ubiquitin ligase complex induicing HSF1 degradation, and hence mitotic progression (PubMed:18794143). Regulates mitotic progression by phosphorylating RIOK2 (PubMed:21880710).

Alternative names

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Recombinant human PLK1 protein is a Human Full Length protein, in the 1 to 603 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for WB, FuncS, SDS-PAGE, IHC-P.

Alternative names

Key facts

Purity

>70%

Expression system

Baculovirus infected Sf9 cells

Applications

WB, FuncS, SDS-PAGE, IHC-P

Biological activity

Specific activity 17nmol/min/mg.

Accession
P53350-1
Animal free

No

Species

Human

Concentration
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Storage buffer

pH: 7
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

Sequence info

Amino acid sequence

M S A A V T A G K L A R A P A D P G K A G V P G V A A P G A P A A A P P A K E I P E V L V D P R S R R R Y V R G R F L G K G G F A K C F E I S D A D T K E V F A G K I V P K S L L L K P H Q R E K M S M E I S I H R S L A H Q H V V G F H G F F E D N D F V F V V L E L C R R R S L L E L H K R R K A L T E P E A R Y Y L R Q I V L G C Q Y L H R N R V I H R D L K L G N L F L N E D L E V K I G D F G L A T K V E Y D G E R K K T L C G T P N Y I A P E V L S K K G H S F E V D V W S I G C I M Y T L L V G K P P F E T S C L K E T Y L R I K K N E Y S I P K H I N P V A A S L I Q K M L Q T D P T A R P T I N E L L N D E F F T S G Y I P A R L P I T C L T I P P R F S I A P S S L D P S N R K P L T V L N K G L E N P L P E R P R E K E E P V V R E T G E V V D C H L S D M L Q Q L H S V N A S K P S E R G L V R Q E E A E D P A C I P I F W V S K W V D Y S D K Y G L G Y Q L C D N S V G V L F N D S T R L I L Y N D G D S L Q Y I E R D G T E S Y L T V S S H P N S L M K K I T L L K Y F R N Y M S E H L L K A G A N I T P R E G D E L A R L P Y L R T W F R T R S A I I L H L S N G S V Q I N F F Q D H T K L I L C P L M A A V T Y I D E K R D F R T Y R L S L L E E Y G C C K E L A S R L R Y A R T M V D K L L S S R S A S N R L K A S

Accession

P53350

Protein length

Full Length

Predicted molecular weight

70 kDa

Amino acids

1 to 603

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

Purified by affinity chromatography

General info

Function

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGO1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, WEE1 and HNRNPU. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Phosphorylation of NINL component of the centrosome leads to NINL dissociation from other centrosomal proteins. Involved in mitosis exit and cytokinesis by phosphorylating CEP55, ECT2, KIF20A/MKLP2, CENPU, PRC1 and RACGAP1. Recruited at the central spindle by phosphorylating and docking PRC1 and KIF20A/MKLP2; creates its own docking sites on PRC1 and KIF20A/MKLP2 by mediating phosphorylation of sites subsequently recognized by the POLO box domains. Phosphorylates RACGAP1, thereby creating a docking site for the Rho GTP exchange factor ECT2 that is essential for the cleavage furrow formation. Promotes the central spindle recruitment of ECT2. Plays a central role in G2/M transition of mitotic cell cycle by phosphorylating CCNB1, CDC25C, FOXM1, CENPU, PKMYT1/MYT1, PPP1R12A/MYPT1 and WEE1. Part of a regulatory circuit that promotes the activation of CDK1 by phosphorylating the positive regulator CDC25C and inhibiting the negative regulators WEE1 and PKMYT1/MYT1. Also acts by mediating phosphorylation of cyclin-B1 (CCNB1) on centrosomes in prophase. Phosphorylates FOXM1, a key mitotic transcription regulator, leading to enhance FOXM1 transcriptional activity. Involved in kinetochore functions and sister chromatid cohesion by phosphorylating BUB1B/BUBR1, FBXO5/EMI1 and STAG2/SA2. PLK1 is high on non-attached kinetochores suggesting a role of PLK1 in kinetochore attachment or in spindle assembly checkpoint (SAC) regulation. Required for kinetochore localization of BUB1B. Regulates the dissociation of cohesin from chromosomes by phosphorylating cohesin subunits such as STAG2/SA2. Phosphorylates SGO1: required for spindle pole localization of isoform 3 of SGO1 and plays a role in regulating its centriole cohesion function. Mediates phosphorylation of FBXO5/EMI1, a negative regulator of the APC/C complex during prophase, leading to FBXO5/EMI1 ubiquitination and degradation by the proteasome. Acts as a negative regulator of p53 family members: phosphorylates TOPORS, leading to inhibit the sumoylation of p53/TP53 and simultaneously enhance the ubiquitination and subsequent degradation of p53/TP53. Phosphorylates the transactivation domain of the transcription factor p73/TP73, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Also required for recovery after DNA damage checkpoint and entry into mitosis. Phosphorylates MISP, leading to stabilization of cortical and astral microtubule attachments required for proper spindle positioning (PubMed:8991084, PubMed:11202906, PubMed:12207013, PubMed:12447691, PubMed:12524548, PubMed:12738781, PubMed:12852856, PubMed:12939256, PubMed:14532005, PubMed:14734534, PubMed:15070733, PubMed:15148369, PubMed:15469984, PubMed:16198290, PubMed:16247472, PubMed:16980960, PubMed:17081991, PubMed:17351640, PubMed:17376779, PubMed:17617734, PubMed:18174154, PubMed:18331714, PubMed:18418051, PubMed:18477460, PubMed:18521620, PubMed:18615013, PubMed:19160488, PubMed:19351716, PubMed:19468300, PubMed:19468302, PubMed:19473992, PubMed:19509060, PubMed:19597481, PubMed:23455478, PubMed:23509069). Together with MEIKIN, acts as a regulator of kinetochore function during meiosis I: required both for mono-orientation of kinetochores on sister chromosomes and protection of centromeric cohesin from separase-mediated cleavage (By similarity). Phosphorylates CEP68 and is required for its degradation (PubMed:25503564). Regulates nuclear envelope breakdown during prophase by phosphorylating DCTN1 resulting in its localization in the nuclear envelope (PubMed:20679239). Phosphorylates the heat shock transcription factor HSF1, promoting HSF1 nuclear translocation upon heat shock (PubMed:15661742). Phosphorylates HSF1 also in the early mitotic period; this phosphorylation regulates HSF1 localization to the spindle pole, the recruitment of the SCF(BTRC) ubiquitin ligase complex induicing HSF1 degradation, and hence mitotic progression (PubMed:18794143). Regulates mitotic progression by phosphorylating RIOK2 (PubMed:21880710).

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.

Post-translational modifications

Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase. Dephosphorylation at Thr-210 at centrosomes is probably mediated by protein phosphatase 1C (PP1C), via interaction with PPP1R12A/MYPT1. Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint. Phosphorylated in vitro by STK10.

Subcellular localisation

Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome, Spindle

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

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5 product images

  • Functional Studies - Recombinant human PLK1 protein (ab51426), expandable thumbnail

    Functional Studies - Recombinant human PLK1 protein (ab51426)

    The specific activity of PLK1 (ab51426) was determined to be 16.5 nmol/min/mg as per activity assay protocol

  • SDS-PAGE - Recombinant human PLK1 protein (ab51426), expandable thumbnail

    SDS-PAGE - Recombinant human PLK1 protein (ab51426)

    SDS PAGE analysis of ab51426

  • Functional Studies - Recombinant human PLK1 protein (ab51426), expandable thumbnail

    Functional Studies - Recombinant human PLK1 protein (ab51426)

    Kinase activity assay. Specific activity 17 nmol/min/mg.

  • SDS-PAGE - Recombinant human PLK1 protein (ab51426), expandable thumbnail

    SDS-PAGE - Recombinant human PLK1 protein (ab51426)

    Recombiant human PLK1 protein (His tagged). Molecular weight 70 kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant human PLK1 protein (ab51426), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant human PLK1 protein (ab51426)

    Immunohistochemistry of Human Lymphoma tissue staining PLK1 with ab51426 at 1/40. Control is treated with fusion protein.

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