Recombinant human PLK3 protein
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Recombinant human PLK3 protein is a Human Fragment protein, in the 57 to 340 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, WB, FuncS.
View Alternative Names
CNK, FNK, PRK, PLK3, Serine/threonine-protein kinase PLK3, Cytokine-inducible serine/threonine-protein kinase, FGF-inducible kinase, Polo-like kinase 3, Proliferation-related kinase, PLK-3
- FuncS
Unknown
Functional Studies - Recombinant human PLK3 protein (AB125627)
The specific activity of ab125627 was determined to be 2.2 nmol/min/mg.
- FuncS
Unknown
Functional Studies - Recombinant human PLK3 protein (AB125627)
The specific activity of PLK3 (ab125627) was determined to be 9 nmol/min/mg as per activity assay protocol and was equivalent to 2.2 nmol/min/mg as per radiometric assay
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human PLK3 protein (AB125627)
SDS-PAGe of Human PLK3 protein.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human PLK3 protein (AB125627)
SDS PAGE analysis of ab125627
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation : required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress : rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle : part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Post-translational modifications
Phosphorylated in an ATM-dependent manner following DNA damage. Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
Subcellular localisation
Nucleus
Target data
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Product promise
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