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AB171481

Recombinant Human PLSCR3 protein (denatured)

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Recombinant Human PLSCR3 protein (denatured) is a Human Fragment protein, in the 1 to 265 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Phospholipid scramblase 3, PL scramblase 3, Ca(2+)-dependent phospholipid scramblase 3, PLSCR3

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SDS-PAGE - Recombinant Human PLSCR3 protein (denatured) (AB171481)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PLSCR3 protein (denatured) (AB171481)

15% SDS-PAGE analysis of ab171481 (3µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NRY6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAGYLPPKGYAPSPPPPYPVTPGYPEPALHPGPGQAPVPAQVPAPAPGFALFPSPGPVALGSAAPFLPLPGVPSGLEFLVQIDQILIHQKAERVETFLGWETCNRYELRSGAGQPLGQAAEESNCCARLCCGARRPLRVRLADPGDREVLRLLRPLHCGCSCCPCGLQEMEVQAPPGTTIGHVLQTWHPFLPKFSIQDADRQTVLRVVGPCWTCGCGTDTNFEVKTRDESRSVGRISKQWGGLVREALTDADDFGLQFPLDLDVR","proteinLength":"Fragment","predictedMolecularWeight":"30.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":265,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NRY6","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PLSCR3 also known as phospholipid scramblase 3 plays a role in the translocation of phospholipids across cell membranes. It weighs approximately 37 kDa and is closely associated with the mitoplast. This protein is found mainly in the mitochondria heart liver and skeletal muscle tissues. PLSCR3 is vital for maintaining the asymmetry of phospholipid distribution in cellular membranes important for cell viability.
Biological function summary

The activity of PLSCR3 in the mitochondria is significant in apoptosis and lipid metabolism processes. As a part of the scramblase family it collaborates with proteins in the Bcl-2 family to modulate apoptosis. This interaction is important for regulating the release of cytochrome c an important step in the intrinsic pathway of apoptosis. The phospholipid redistribution function of PLSCR3 supports the remodeling of mitochondrial membranes during metabolic processes.

Pathways

PLSCR3 influences the apoptosis and phospholipid signaling pathways. It interacts with proteins such as Bax and Bak within the apoptosis pathway facilitating the proper execution of cell death during stress responses. In the phospholipid signaling pathway PLSCR3 coordinates with phosphatidylserine and other lipids to regulate cellular signaling processes and membrane dynamics affecting cellular responses to environmental changes.

Mutations or dysregulation of PLSCR3 can contribute to metabolic disorders and certain types of cancer. The protein's role in apoptosis links it to disorders like cardiomyopathy where regulated cell death is important for heart muscle maintenance. Additionally in cancer abnormal PLSCR3 activity impacts cell growth and survival connecting it to oncogenes like MYC which can drive uncontrolled cell proliferation. Understanding PLSCR3's role helps develop potential therapeutic strategies for these conditions.
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Specifications

Form

Liquid

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General info

Function

Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of the phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer membrane of the mitochondria (PubMed : 14573790, PubMed : 17226776, PubMed : 18358005, PubMed : 29337693, PubMed : 31769662). Plays an important role in mitochondrial respiratory function, morphology, and apoptotic response (PubMed : 12649167, PubMed : 14573790, PubMed : 17226776, PubMed : 18358005). Mediates the translocation of cardiolipin from the mitochondrial inner membrane to outer membrane enhancing t-Bid induced cytochrome c release and apoptosis (PubMed : 14573790, PubMed : 17226776, PubMed : 18358005). Enhances TNFSF10-induced apoptosis by regulating the distribution of cardiolipin in the mitochondrial membrane resulting in increased release of apoptogenic factors and consequent amplification of the activity of caspases (PubMed : 18491232). Regulates cardiolipin de novo biosynthesis and its resynthesis (PubMed : 16939411).

Sequence similarities

Belongs to the phospholipid scramblase family.

Post-translational modifications

Phosphorylation at Thr-21 by PKC/PRKCD upon apoptotic stimuli enhances phospholipid scramblase activity.. Palmitoylation regulates its localization to the cell membrane or the nucleus; trafficking to the cell membrane is dependent upon palmitoylation whereas in the absence of palmitoylation, localizes to the nucleus.

Subcellular localisation

Mitochondrion membrane

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Product protocols

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Target data

Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of the phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer membrane of the mitochondria (PubMed : 14573790, PubMed : 17226776, PubMed : 18358005, PubMed : 29337693, PubMed : 31769662). Plays an important role in mitochondrial respiratory function, morphology, and apoptotic response (PubMed : 12649167, PubMed : 14573790, PubMed : 17226776, PubMed : 18358005). Mediates the translocation of cardiolipin from the mitochondrial inner membrane to outer membrane enhancing t-Bid induced cytochrome c release and apoptosis (PubMed : 14573790, PubMed : 17226776, PubMed : 18358005). Enhances TNFSF10-induced apoptosis by regulating the distribution of cardiolipin in the mitochondrial membrane resulting in increased release of apoptogenic factors and consequent amplification of the activity of caspases (PubMed : 18491232). Regulates cardiolipin de novo biosynthesis and its resynthesis (PubMed : 16939411).
See full target information PLSCR3
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Complementary Products

Primary Antibodies

AB137128

Anti-PLSCR3 antibody [EPR9242]

RabMAb|Recombinant

Western blot - Anti-PLSCR3 antibody [EPR9242] (AB137128)

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