Recombinant Human PLSCR3 protein (denatured) is a Human Fragment protein, in the 1 to 265 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A G Y L P P K G Y A P S P P P P Y P V T P G Y P E P A L H P G P G Q A P V P A Q V P A P A P G F A L F P S P G P V A L G S A A P F L P L P G V P S G L E F L V Q I D Q I L I H Q K A E R V E T F L G W E T C N R Y E L R S G A G Q P L G Q A A E E S N C C A R L C C G A R R P L R V R L A D P G D R E V L R L L R P L H C G C S C C P C G L Q E M E V Q A P P G T T I G H V L Q T W H P F L P K F S I Q D A D R Q T V L R V V G P C W T C G C G T D T N F E V K T R D E S R S V G R I S K Q W G G L V R E A L T D A D D F G L Q F P L D L D V R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of the phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer membrane of the mitochondria (PubMed:14573790, PubMed:17226776, PubMed:18358005, PubMed:29337693, PubMed:31769662). Plays an important role in mitochondrial respiratory function, morphology, and apoptotic response (PubMed:12649167, PubMed:14573790, PubMed:17226776, PubMed:18358005). Mediates the translocation of cardiolipin from the mitochondrial inner membrane to outer membrane enhancing t-Bid induced cytochrome c release and apoptosis (PubMed:14573790, PubMed:17226776, PubMed:18358005). Enhances TNFSF10-induced apoptosis by regulating the distribution of cardiolipin in the mitochondrial membrane resulting in increased release of apoptogenic factors and consequent amplification of the activity of caspases (PubMed:18491232). Regulates cardiolipin de novo biosynthesis and its resynthesis (PubMed:16939411).
Alternative names
Phospholipid scramblase 3, PL scramblase 3, Ca(2+)-dependent phospholipid scramblase 3, PLSCR3
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Recombinant Human PLSCR3 protein (denatured) is a Human Fragment protein, in the 1 to 265 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9NRY6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A G Y L P P K G Y A P S P P P P Y P V T P G Y P E P A L H P G P G Q A P V P A Q V P A P A P G F A L F P S P G P V A L G S A A P F L P L P G V P S G L E F L V Q I D Q I L I H Q K A E R V E T F L G W E T C N R Y E L R S G A G Q P L G Q A A E E S N C C A R L C C G A R R P L R V R L A D P G D R E V L R L L R P L H C G C S C C P C G L Q E M E V Q A P P G T T I G H V L Q T W H P F L P K F S I Q D A D R Q T V L R V V G P C W T C G C G T D T N F E V K T R D E S R S V G R I S K Q W G G L V R E A L T D A D D F G L Q F P L D L D V R
- Accession
- Q9NRY6
- Protein length
- Fragment
- Predicted molecular weight
- 30.9 kDa
- Amino acids
- 1 to 265
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of the phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer membrane of the mitochondria (PubMed:14573790, PubMed:17226776, PubMed:18358005, PubMed:29337693, PubMed:31769662). Plays an important role in mitochondrial respiratory function, morphology, and apoptotic response (PubMed:12649167, PubMed:14573790, PubMed:17226776, PubMed:18358005). Mediates the translocation of cardiolipin from the mitochondrial inner membrane to outer membrane enhancing t-Bid induced cytochrome c release and apoptosis (PubMed:14573790, PubMed:17226776, PubMed:18358005). Enhances TNFSF10-induced apoptosis by regulating the distribution of cardiolipin in the mitochondrial membrane resulting in increased release of apoptogenic factors and consequent amplification of the activity of caspases (PubMed:18491232). Regulates cardiolipin de novo biosynthesis and its resynthesis (PubMed:16939411).
- Sequence similarities
Belongs to the phospholipid scramblase family.
- Post-translational modifications
Phosphorylation at Thr-21 by PKC/PRKCD upon apoptotic stimuli enhances phospholipid scramblase activity.
- Subcellular localisation
- Mitochondrion membrane, Mitochondrion inner membrane, Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PLSCR3 also known as phospholipid scramblase 3 plays a role in the translocation of phospholipids across cell membranes. It weighs approximately 37 kDa and is closely associated with the mitoplast. This protein is found mainly in the mitochondria heart liver and skeletal muscle tissues. PLSCR3 is vital for maintaining the asymmetry of phospholipid distribution in cellular membranes important for cell viability.
- Biological function summary
The activity of PLSCR3 in the mitochondria is significant in apoptosis and lipid metabolism processes. As a part of the scramblase family it collaborates with proteins in the Bcl-2 family to modulate apoptosis. This interaction is important for regulating the release of cytochrome c an important step in the intrinsic pathway of apoptosis. The phospholipid redistribution function of PLSCR3 supports the remodeling of mitochondrial membranes during metabolic processes.
- Pathways
PLSCR3 influences the apoptosis and phospholipid signaling pathways. It interacts with proteins such as Bax and Bak within the apoptosis pathway facilitating the proper execution of cell death during stress responses. In the phospholipid signaling pathway PLSCR3 coordinates with phosphatidylserine and other lipids to regulate cellular signaling processes and membrane dynamics affecting cellular responses to environmental changes.
- Associated diseases and disorders
Mutations or dysregulation of PLSCR3 can contribute to metabolic disorders and certain types of cancer. The protein's role in apoptosis links it to disorders like cardiomyopathy where regulated cell death is important for heart muscle maintenance. Additionally in cancer abnormal PLSCR3 activity impacts cell growth and survival connecting it to oncogenes like MYC which can drive uncontrolled cell proliferation. Understanding PLSCR3's role helps develop potential therapeutic strategies for these conditions.
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1 product image
SDS-PAGE - Recombinant Human PLSCR3 protein (denatured) (ab171481)
15% SDS-PAGE analysis of ab171481 (3µg).
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