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AB132814

Recombinant Human PM20D1 protein

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Recombinant Human PM20D1 protein is a Human Full Length protein, in the 1 to 502 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

N-fatty-acyl-amino acid synthase/hydrolase PM20D1, Peptidase M20 domain-containing protein 1, PM20D1

1 Images
SDS-PAGE - Recombinant Human PM20D1 protein (AB132814)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PM20D1 protein (AB132814)

12.5% SDS-PAGE analysis of ab132814 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

Q6GTS8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAQRCVCVLALVAMLLLVFPTVSRSMGPRSGEYQRASRIPSQFSKEERVAMKEALKGAIQIPTVTFSSEKSNTTALAEFGKYIRKVFPTVVSTSFIQHEVVEEYSHLFTIQGSDPSLQPYLLMAHFDVVPAPEEGWEVPPFSGLERDGVIYGRGTLDDKNSVMALLQALELLLIRKYIPRRSFFISLGHDEESSGTGAQRISALLQSRGVQLAFIVDEGGFILDDFIPNFKKPIALTAVSEKGSMNLMLQVNMTSGHSSAPPKETSIGILAAAVSRLEQTPMPIIFGSGTVVTVLQQLANEFPFPVNIILSNPWLFEPLISRFMERNPLTNAIIRTTTALTIFKARVKFNVIPPVAQATVNFRIHPGQTVQEVLELTKNTVADNRVQFHVLSAFDPLPVSPSDDKALGYQLLRQTVQSVFPEVNITAPVTSIGNTDSRFFTNLTTGIYRFYPIYIQPEDFKRIHGVNEKISVQAYETQVKFIFELIQNADTDQEPVSHLHKL","proteinLength":"Full Length","predictedMolecularWeight":"82.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":502,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q6GTS8","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PM20D1 also called peptidase M20 domain containing 1 functions as an enzyme with a significant role in metabolism. It is part of the M20 peptidase family and has a molecular weight of about 59 kDa. PM20D1 is expressed in various tissues with high levels in adipose tissue and brown fat pointing to its involvement in energy metabolism. This enzyme has involvement in hydrolyzing specific peptide bonds which is critical for regulating lipid metabolism.
Biological function summary

PM20D1 is involved in the regulation of thermogenic processes and energy expenditure. It acts as an amidase facilitating the transformation of N-acyl amino acids. These metabolites can influence mitochondrial uncoupling impacting energy dissipation as heat. Although PM20D1 operates independently its activity can affect the larger metabolic network that maintains energy balance in the body.

Pathways

PM20D1 contributes significantly to the metabolic pathways of fatty acid oxidation and thermogenesis. It influences the presence of metabolites such as N-acyl amino acids which interact with proteins like UCP1 (uncoupling protein 1) pivotal in creating heat in brown adipose tissue. However PM20D1 does not form part of a specific protein complex but modulates pathways that are intricate in energy metabolism.

PM20D1 links to obesity and type 2 diabetes as its metabolic role influences energy homeostasis and insulin sensitivity. Alterations in the expression or function of PM20D1 can impact adipose tissue activity potentially leading to metabolic dysfunctions. Connections with proteins such as UCP1 solidify its relevance in metabolic disorders where impaired energy dissipation leads to increased fat storage highlighting its importance in researching metabolic health and disease.
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Specifications

Form

Liquid

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General info

Function

Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase (PubMed : 27374330). It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction (PubMed : 27374330). Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles (By similarity).

Sequence similarities

Belongs to the peptidase M20A family.

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Product protocols

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Target data

Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase (PubMed : 27374330). It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction (PubMed : 27374330). Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles (By similarity).
See full target information PM20D1
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