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AB314595

Recombinant Human PMCH protein (His Tag)

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Recombinant Human PMCH protein (His Tag) is a Human Full Length protein, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE.

View Alternative Names

MCH, PMCH, Pro-MCH

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SDS-PAGE - Recombinant Human PMCH protein (His Tag) (AB314595)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PMCH protein (His Tag) (AB314595)

Coomassie blue staining of purified ab314595 expressed in E. coli.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P20382

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7 Constituents: 1% Tris HCl, 0.1% Urea

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"18.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P20382","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle|The product can be stored for up to 12 months
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Melanin-concentrating hormone (MCH) originally isolated from fish is a peptide hormone that plays a significant role in energy balance and feeding behavior. It is about 19 amino acids long in humans and has a molecular mass of approximately 2.4 kDa. MCH is mainly expressed in the lateral hypothalamus and zona incerta of the brain which suggests its central role in neurologic function. This peptide binds to MCH receptor 1 (MCHR1) influencing various signaling pathways.
Biological function summary

MCH affects a range of physiological processes and is part of the neuropeptide signaling system. It regulates feeding and energy homeostasis by influencing appetite increase and reducing energy expenditure. MCH operates within a complex network of neurotransmitters and hormones in the central nervous system. Its interactions with other neuropeptides such as neuropeptide Y (NPY) and agouti-related peptide (AgRP) support its role in the complex regulation of energy homeostasis.

Pathways

MCH engages in signaling linked to G-protein coupled receptors (GPCRs) connecting with pathways that regulate food intake and energy management. It influences the orexin/hypocretin pathway which also modulates sleep-wake cycles and arousal states. The interaction with orexin highlights MCH's broad regulatory influence balancing energy levels and adaptive responses. The protein's signaling is associated with molecules like leptin and insulin marking its integration into wider metabolic pathways.

MCH is linked to obesity and mood disorders due to its influence on appetite and emotional regulation. A dysregulated MCH signaling pathway is associated with overeating and weight gain highlighting its role in obesity physiology. Additionally altered levels of MCH could contribute to mood disorders such as depression where its interaction with serotonin and dopamine pathways may impact emotional stability.
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Specifications

Form

Liquid

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General info

Function

MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.

Sequence similarities

Belongs to the melanin-concentrating hormone family.

Post-translational modifications

Differentially processed in the brain and in peripheral organs producing two neuropeptides; NEI and MCH. A third peptide, NGE, may also be produced. Preferential processing in neurons by prohormone convertase 2 (PC2) generates NEI. MCH is generated in neurons of the lateral hypothalmic area by several prohormone convertases including PC1/3, PC2 and PC5/6.

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Product protocols

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Target data

MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.
See full target information PMCH
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Assay Kits

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Product promise

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