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AB109945

Recombinant Human PME-1 protein

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Recombinant Human PME-1 protein is a Human Full Length protein, in the 1 to 386 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PME1, PP2593, PRO0750, PPME1, Protein phosphatase methylesterase 1, PME-1

1 Images
SDS-PAGE - Recombinant Human PME-1 protein (AB109945)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PME-1 protein (AB109945)

15% SDS-PAGE analysis of 3μg ab109945.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9Y570

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.058% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as PPME1.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC","proteinLength":"Full Length","predictedMolecularWeight":"44.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":386,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y570","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase methylesterase 1 (PME-1) also known as protein phosphatase 2A methylesterase is an enzyme that regulates protein function by demethylation. PME-1 has a mass of approximately 42 kDa. It primarily resides in the cytoplasm and nucleus of cells with high expression levels in brain tissue. Its function involves the removal of methyl groups from the carboxyl-terminal leucine residue of the catalytic subunit of protein phosphatase 2A (PP2A). This action regulates the activity states of PP2A a major serine/threonine phosphatase.
Biological function summary

PME-1 influences the stability and activity of PP2A a core component of cellular signal transduction pathways. PME-1 forms part of the regulatory complexes of PP2A modulating its methylation state and associated functional properties. This regulation by PME-1 impacts cell cycle control apoptosis and other critical cellular processes. Additionally PME-1 activity affects signaling pathways by altering PP2A's interaction with various substrates and regulatory proteins.

Pathways

The role of PME-1 in cellular mechanisms links it to the Wnt signaling pathway and MAP kinase signaling cascade. PME-1 catalyzes demethylation reactions that impact PP2A's ability to associate with specific regulatory subunits. Through the Wnt pathway PME-1 affects cellular processes such as proliferation and differentiation by regulating beta-catenin activity. In relation to the MAP kinase pathway PME-1 influences cell stress response and growth factor signaling interacting with proteins like ERK and p38 MAP kinase.

PME-1 has associations with cancer and neurodegenerative conditions. In cancer altered PME-1 expression or activity can lead to uncontrolled cell growth and survival acting through PP2A-related pathways. In neurodegenerative diseases such as Alzheimer's PME-1 may affect tau protein phosphorylation by modulating PP2A activity potentially contributing to pathogenesis. As PP2A regulates tau phosphorylation disruptions in its demethylation by PME-1 has been correlated with disease progression.
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Specifications

Form

Liquid

Additional notes

ab109945 was purified using conventional chromatography.

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General info

Function

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.

Sequence similarities

Belongs to the AB hydrolase superfamily.

Post-translational modifications

Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1.

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Product protocols

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Target data

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.
See full target information PPME1
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Complementary Products

Primary Antibodies

AB205956

Anti-PME-1 antibody

Immunoprecipitation - Anti-PME-1 antibody (AB205956)

  • 0
  • 0
Primary Antibodies

AB128006

Anti-TMEPAI antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEPAI antibody (AB128006)

  • 0
  • 0
Primary Antibodies

AB183518

Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal

RabMAb|Recombinant

Western blot - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (AB183518)

  • 0
  • 0
Primary Antibodies

AB198171

Anti-NOC2L antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOC2L antibody (AB198171)

  • 0
  • 0

Product promise

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