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AB99391

Recombinant Human PMM2 protein

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Recombinant Human PMM2 protein is a Human Full Length protein, in the 1 to 246 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Phosphomannomutase 2, PMM 2, PMM2

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SDS-PAGE - Recombinant Human PMM2 protein (AB99391)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PMM2 protein (AB99391)

15% SDS-PAGE analysis of 3μg ab99391.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

O15305

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAAPGPALCLFDVDGTLTAPRQKITKEMDDFLQKLRQKIKIGVVGGSDFEKVQEQLGNDVVEKYDYVFPENGLVAYKDGKLLCRQNIQSHLGEALIQDLINYCLSYIAKIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKENIRQKFVADLRKEFAGKGLTFSIGGQISFDVFPDGWDKRYCLRHVENDGYKTIYFFGDKTMPGGNDHEIFTDPRTMGYSVTAPEDTRRICELLFS","proteinLength":"Full Length","predictedMolecularWeight":"30.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":246,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O15305","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphomannomutase 2 (PMM2) also known as PMM 22 or PMM II is an enzyme that plays a role in the conversion of mannose-6-phosphate to mannose-1-phosphate. PMM2 is about 29 kDa in size and exists predominantly in the cytoplasm of cells. It shows widespread expression but higher levels are found in the liver brain and muscle tissues. PMM2 activity is important in carbohydrate metabolism making it an enzyme of interest especially in the context of glycobiology and metabolic research.
Biological function summary

PMM2 catalyzes an important step in the biosynthesis of GDP-mannose which is necessary for glycan production. These glycans are essential in the process of glycosylation a post-translational modification of proteins and lipids. PMM2 acts independently and does not form part of a larger protein complex. Through its role in glycan synthesis PMM2 impacts cell signaling protein folding and molecular interactions.

Pathways

PMM2 is involved in the N-glycosylation pathway and the GDP-mannose biosynthesis pathway. It collaborates closely with proteins such as PMM1 as both contribute to mannose metabolism and glycan assembly. PMM2 is significant in these pathways as it impacts not only the formation of sugar nucleotides but also their integration into larger biomolecules.

PMM2 dysfunction is directly linked to Congenital Disorders of Glycosylation Type Ia (CDG-Ia). This genetic disorder results from mutations in the PMM2 gene impairing glycosylation processes and resulting in multisystemic clinical manifestations. The protein PMM1 while not directly associated with the disorder serves complementary functions in mannose phosphate isomerization and may modulate the impact of PMM2 deficiencies.
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Specifications

Form

Liquid

Additional notes

ab99391 is purified using conventional chromatography techniques.

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General info

Function

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

Sequence similarities

Belongs to the eukaryotic PMM family.

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Product protocols

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Target data

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
See full target information PMM2
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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB229996

Anti-PMM2 antibody

Western blot - Anti-PMM2 antibody (AB229996)

  • 0
  • 0
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB99695

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain

  • 0
  • 0

Product promise

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