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AB165200

Recombinant Human PNAD protein

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Recombinant Human PNAD protein is a Human Full Length protein, in the 1 to 310 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Protein N-terminal asparagine amidohydrolase, Protein NH2-terminal asparagine amidohydrolase, Protein NH2-terminal asparagine deamidase, PNAA, PNAD, Protein N-terminal Asn amidase, Protein N-terminal asparagine amidase, Protein NTN-amidase, NTAN1

1 Images
SDS-PAGE - Recombinant Human PNAD protein (AB165200)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PNAD protein (AB165200)

ab165200 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q96AB6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as NTAN1.
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Sequence info

[{"sequence":"MPLLVEGRRVRLPQSAGDLVRAHPPLEERARLLRGQSVQQVGPQGLLYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGTDTKAEVPLIMNSIKSFSDHAQCGRLEVHLVGGFSDDRQLSQKLTHQLLSEFDRQEDDIHLVTLCVTELNDREENENHFPVIYGIAVNIKTAEIYRASFQDRGPEEQLRAARTLAGGPMISIYDAETEQLRIGPYSWTPFPHVDFWLHQDDKQILENLSTSPLAEPPHFVEHIRSTLMFLKKHPSPAHTLFSGNKALLYKKNEDGLWEKISSPGS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":310,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96AB6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Peripheral Node Addressin (PNAd) is a glycoprotein recognized by its role in lymphocyte homing to lymph nodes. It is an important ligand for L-selectin on leukocytes facilitating their entry into lymphoid tissues. The PNAd marker is primarily located on the high endothelial venules (HEVs) where it guides lymphocyte trafficking. PNAd's molecular weight varies depending on its glycosylation patterns but is generally documented around 50 to 90 kDa.
Biological function summary

PNAd mediates the adhesion of lymphocytes to HEVs a critical step in the immune response promoting the recirculation of lymphocytes throughout the body. PNAd does not function as an independent entity often part of a multiprotein complex involving various glycoproteins and ligands that collectively ensure efficient immune surveillance. Its interaction with L-selectin is an essential event in lymphocyte adhesion under shear flow conditions seen in blood circulation.

Pathways

PNAd plays a role in the lymphocyte signaling pathways particularly associated with leukocyte extravasation. This pathway is integral for lymphocyte migration involving key signaling molecules and interactions with proteins like ICAM-1 and VCAM-1. PNAd's function in this pathway reflects its importance for maintaining effective immune system balance and proper lymphocyte distribution.

PNAd has involvement in inflammatory conditions such as rheumatoid arthritis and various lymphomas where aberrant lymphocyte trafficking occurs. In rheumatoid arthritis unusual PNAd expression on inflamed venules can lead to inappropriate lymphocyte accumulation in joints. Similarly in some lymphomas altered PNAd expression may associate with changes in lymphocyte migration patterns. Its interaction with proteins such as L-selectin occurs more frequently in these pathologies often exacerbating disease profiles.
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Specifications

Form

Liquid

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General info

Function

N-terminal asparagine deamidase that mediates deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by NTAN1/PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn.

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Product protocols

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Target data

N-terminal asparagine deamidase that mediates deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by NTAN1/PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn.
See full target information NTAN1
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