Recombinant Human PNK/PNKP protein is a Human Full Length protein, in the 1 to 521 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M G E V E A P G R L W L E S P P G G A P P I F L P S D G Q A L V L G R G P L T Q V T D R K C S R T Q V E L V A D P E T R T V A V K Q L G V N P S T T G T Q E L K P G L E G S L G V G D T L Y L V N G L H P L T L R W E E T R T P E S Q P D T P P G T P L V S Q D E K R D A E L P K K R M R K S N P G W E N L E K L L V F T A A G V K P Q G K V A G F D L D G T L I T T R S G K V F P T G P S D W R I L Y P E I P R K L R E L E A E G Y K L V I F T N Q M S I G R G K L P A E E F K A K V E A V V E K L G V P F Q V L V A T H A G L Y R K P V T G M W D H L Q E Q A N D G T P I S I G D S I F V G D A A G R P A N W A P G R K K K D F S C A D R L F A L N L G L P F A T P E E F F L K W P A A G F E L P A F D P R T V S R S G P L C L P E S R A L L S A S P E V V V A V G F P G A G K S T F L K K H L V S A G Y V H V N R D T L G S W Q R C V T T C E T A L K Q G K R V A I D N T N P D A A S R A R Y V Q C A R A A G V P C R C F L F T A T L E Q A R H N N R F R E M T D S S H I P V S D M V M Y G Y R K Q F E A P T L A E G F S A I L E I P F R L W V E P R L G R L Y C Q F S E G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes (Recombinant protein). |
Target data
Function
Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways (PubMed:10446192, PubMed:10446193, PubMed:15385968, PubMed:20852255, PubMed:28453785). Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone (PubMed:10446192, PubMed:10446193).
Alternative names
Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, PNKP
Recommended products
Recombinant Human PNK/PNKP protein is a Human Full Length protein, in the 1 to 521 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q96T60-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M G E V E A P G R L W L E S P P G G A P P I F L P S D G Q A L V L G R G P L T Q V T D R K C S R T Q V E L V A D P E T R T V A V K Q L G V N P S T T G T Q E L K P G L E G S L G V G D T L Y L V N G L H P L T L R W E E T R T P E S Q P D T P P G T P L V S Q D E K R D A E L P K K R M R K S N P G W E N L E K L L V F T A A G V K P Q G K V A G F D L D G T L I T T R S G K V F P T G P S D W R I L Y P E I P R K L R E L E A E G Y K L V I F T N Q M S I G R G K L P A E E F K A K V E A V V E K L G V P F Q V L V A T H A G L Y R K P V T G M W D H L Q E Q A N D G T P I S I G D S I F V G D A A G R P A N W A P G R K K K D F S C A D R L F A L N L G L P F A T P E E F F L K W P A A G F E L P A F D P R T V S R S G P L C L P E S R A L L S A S P E V V V A V G F P G A G K S T F L K K H L V S A G Y V H V N R D T L G S W Q R C V T T C E T A L K Q G K R V A I D N T N P D A A S R A R Y V Q C A R A A G V P C R C F L F T A T L E Q A R H N N R F R E M T D S S H I P V S D M V M Y G Y R K Q F E A P T L A E G F S A I L E I P F R L W V E P R L G R L Y C Q F S E G
- Accession
- Q96T60
- Protein length
- Full Length
- Predicted molecular weight
- 83.5 kDa
- Amino acids
- 1 to 521
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways (PubMed:10446192, PubMed:10446193, PubMed:15385968, PubMed:20852255, PubMed:28453785). Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone (PubMed:10446192, PubMed:10446193).
- Sequence similarities
In the N-terminal section; belongs to the DNA 3' phosphatase family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as PNK.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Polyribonucleotide 5'-hydroxyl-kinase 3'-phosphatase often known as PNK or PNKP protein is a dual-function enzyme necessary in DNA repair processes. PNKP with a mass of about 57 kDa performs both kinase and phosphatase activities. It removes 3'-phosphates and phosphorylates 5'-hydroxyl groups in polynucleotides preparing them for subsequent repair. This enzyme is expressed in various tissues with significant levels in the brain indicating its widespread importance in cellular processes.
- Biological function summary
PNKP protein is integral to maintaining genome stability by facilitating the repair of single-strand breaks and double-strand breaks in DNA. It is not part of a complex but often collaborates with other repair proteins through its enzymatic functions. PNKP ensures repaired DNA ends are properly processed to allow accurate ligation by DNA ligases contributing to the integrity of the genetic material in cells.
- Pathways
PNKP operates mainly in the DNA repair pathways specifically in Base Excision Repair (BER) and Non-Homologous End Joining (NHEJ). Within the BER pathway PNKP collaborates with proteins like DNA polymerase β and XRCC1 in processing damaged DNA. In the NHEJ pathway it associates with proteins like Ku and DNA-PKcs aligning for intricate repair of DNA double-strand breaks.
- Associated diseases and disorders
Mutations and malfunctions in PNKP are linked to neurological disorders and cancer. Dysfunctions in PNKP activity associate with neurodegenerative conditions like Microcephaly with Seizures due to its role in efficient DNA repair in neuronal tissues. Additionally its connection to DNA Repair pathways links PNKP malfunction with increased cancer risk. PNKP involvement with proteins like ATM and ATR signals its importance as these are key players in the cellular response to DNA damage particularly in preventing tumorigenesis.
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1 product image
SDS-PAGE - Recombinant Human PNK/PNKP protein (ab131925)
12.5% SDS-PAGE stained with Coomassie Blue showing ab131925.
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