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AB131925

Recombinant Human PNK/PNKP protein (GST tag N-Terminus)

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Recombinant Human PNK/PNKP protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 521 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, PNKP

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SDS-PAGE - Recombinant Human PNK/PNKP protein (GST tag N-Terminus) (AB131925)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PNK/PNKP protein (GST tag N-Terminus) (AB131925)

12.5% SDS-PAGE stained with Coomassie Blue showing ab131925.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q96T60

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB181107

Anti-PNK/PNKP antibody [EPR11935] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (AB181107)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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Product details

This product was previously labelled as PNK.
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Sequence info

[{"sequence":"MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG","proteinLength":"Full Length","predictedMolecularWeight":"83.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":521,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96T60","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Polyribonucleotide 5'-hydroxyl-kinase 3'-phosphatase often known as PNK or PNKP protein is a dual-function enzyme necessary in DNA repair processes. PNKP with a mass of about 57 kDa performs both kinase and phosphatase activities. It removes 3'-phosphates and phosphorylates 5'-hydroxyl groups in polynucleotides preparing them for subsequent repair. This enzyme is expressed in various tissues with significant levels in the brain indicating its widespread importance in cellular processes.
Biological function summary

PNKP protein is integral to maintaining genome stability by facilitating the repair of single-strand breaks and double-strand breaks in DNA. It is not part of a complex but often collaborates with other repair proteins through its enzymatic functions. PNKP ensures repaired DNA ends are properly processed to allow accurate ligation by DNA ligases contributing to the integrity of the genetic material in cells.

Pathways

PNKP operates mainly in the DNA repair pathways specifically in Base Excision Repair (BER) and Non-Homologous End Joining (NHEJ). Within the BER pathway PNKP collaborates with proteins like DNA polymerase β and XRCC1 in processing damaged DNA. In the NHEJ pathway it associates with proteins like Ku and DNA-PKcs aligning for intricate repair of DNA double-strand breaks.

Mutations and malfunctions in PNKP are linked to neurological disorders and cancer. Dysfunctions in PNKP activity associate with neurodegenerative conditions like Microcephaly with Seizures due to its role in efficient DNA repair in neuronal tissues. Additionally its connection to DNA Repair pathways links PNKP malfunction with increased cancer risk. PNKP involvement with proteins like ATM and ATR signals its importance as these are key players in the cellular response to DNA damage particularly in preventing tumorigenesis.
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Specifications

Form

Liquid

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General info

Function

Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways (PubMed : 10446192, PubMed : 10446193, PubMed : 15385968, PubMed : 20852255, PubMed : 28453785). Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone (PubMed : 10446192, PubMed : 10446193).

Sequence similarities

In the N-terminal section; belongs to the DNA 3' phosphatase family.

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Product protocols

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Target data

Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways (PubMed : 10446192, PubMed : 10446193, PubMed : 15385968, PubMed : 20852255, PubMed : 28453785). Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone (PubMed : 10446192, PubMed : 10446193).
See full target information PNKP
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Product promise

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