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AB104634

Recombinant Human pNO40 protein

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Recombinant Human pNO40 protein is a Human Full Length protein, in the 1 to 241 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PS1D, HSPC243, HSPC251, LDC4, ZCCHC17, Zinc finger CCHC domain-containing protein 17, Nucleolar protein of 40 kDa, Pnn-interacting nucleolar protein, Putative S1 RNA-binding domain protein, pNO40, PS1D protein

1 Images
SDS-PAGE - Recombinant Human pNO40 protein (AB104634)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human pNO40 protein (AB104634)

15% SDS-PAGE showing ab104634 (3 μg).
Note : Molecular weight on SDS-PAGE will appear higher than the predicted molecular weight of approximately 30 kDa.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9NP64

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as ZCCHC17.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMNSGRPETMENLPALYTIFQGEVAMVTDYGAFIKIPGCRKQGLVHRTHMSSCRVDKPSEIVDVGDKVWVKLIGREMKNDRIKVSLSMKVVNQGTGKDLDPNNVIIEQEERRRRSFQDYTGQKITLEAVLNTTCKKCGCKGHFAKDCFMQPGGTKYSLIPDEEEEKEEAKSAEFEKPDPTRNPSRKRKKEKKKKKHRDRKSSDSDSSDSESDTGKRARHTSKDSKAAKKKKKKKKHKKKHKE","proteinLength":"Full Length","predictedMolecularWeight":"30 kDa","actualMolecularWeight":null,"aminoAcidEnd":241,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NP64","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PNO40 also known as RPLP0 is a ribosomal protein that plays a mechanical role in the ribonucleoprotein complex responsible for peptide synthesis. It has a molecular mass of about 34 kDa and is ubiquitously expressed across various human tissues especially in the cytoplasm and nucleolus. pNO40 functions as a structural component of the ribosome participating in the assembly and stability of the ribosomal large subunit. The protein is essential for the proper formation of ribosomal RNA and optimal ribosome performance during translation.
Biological function summary

PNO40 engages significantly in ribosomal biogenesis and protein synthesis where it anchors the essential phosphoprotein complex of the ribosome. Its presence ensures that translation occurs effectively influencing cellular protein production. This protein is not part of a complex by itself but often interacts with acidic ribosomal phosphoproteins including RPLP1 and RPLP2 which are important for ribosomal assembly and function. The activity of pNO40 ensures the ribosome's structural integrity critical for maintaining cellular homeostasis.

Pathways

When considering translational machinery pNO40 actively participates in the translation pathway ensuring efficient protein synthesis. It associates with the ribosome-related pathway where it interacts with proteins like EF-G (Elongation Factor G) that catalyze the translocation of the ribosome along mRNA. These interactions are key to understanding how protein synthesis influences cellular response to environmental changes. The functionality of pNO40 within these pathways reflects its foundational role in maintaining cellular functions.

The expression and function of pNO40 have implications for certain types of cancer and anemia. In cancer altered levels of pNO40 can lead to dysregulated protein synthesis contributing to rapid cellular proliferation. This protein interacts with dysregulated pathways that also involve tumor suppressor proteins like p53. In anemia impairment of pNO40 can affect ribosome assembly reducing erythropoiesis. Understanding these interactions is important for potential therapeutic strategies targeting ribosomal biogenesis.
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Specifications

Form

Liquid

Additional notes

ab104634 is purified using conventional chromatography techniques.

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General info

Subcellular localisation

Nucleus

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Product protocols

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Target data

See full target information ZCCHC17
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Complementary Products

Cell Lines & Lysates

AB265206

Human ZCCHC17 (pNO40) knockout HeLa cell line

Sanger Sequencing - Human ZCCHC17 (pNO40) knockout HeLa cell line (AB265206)

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Cell Lines & Lysates

AB258766

Human ZCCHC17 (pNO40) knockout HeLa cell lysate

Sanger Sequencing - Human ZCCHC17 (pNO40) knockout HeLa cell lysate (AB258766)

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